【摘要】：目的探究GDF8及其相關(guān)受體在人正常卵巢及PCOS卵巢卵泡中的表達(dá)。方法用免疫組化方法染色卵巢組織病理切片標(biāo)本,使用顯微鏡評(píng)估GDF8及其受體在細(xì)胞中的定位、表達(dá)強(qiáng)度。結(jié)果1.通過(guò)免疫組化方法發(fā)現(xiàn)GDF8及其受體(ACVR2A、ACVR2B、ALK5)蛋白在正常卵巢及PCOS卵巢的初級(jí)卵泡的部分顆粒細(xì)胞中有表達(dá),在始基卵泡的顆粒細(xì)胞中不表達(dá)。2.無(wú)論在正常卵巢還是PCOS卵巢的生長(zhǎng)卵泡中(從始基卵泡到10mm竇卵泡)中均可看到GDF8及其受體蛋白在卵母細(xì)胞中表達(dá)。3.GDF8及其受體在正常卵巢或者PCOS卵巢0-2mm,2-5mm,5-10mm竇卵泡的顆粒細(xì)胞及卵泡膜細(xì)胞中均有表達(dá),并且隨著竇卵泡直徑的增加,以上蛋白的表達(dá)也逐步顯著增加。4.無(wú)論在正常卵巢還是PCOS卵巢中,在2-5mm及5-1 Omm的竇卵泡中,GDF8及其受體在顆粒細(xì)胞中表達(dá)顯著高于對(duì)應(yīng)的卵泡膜細(xì)胞,在0-2mm竇卵泡中兩種細(xì)胞中表達(dá)強(qiáng)度沒(méi)有顯著性差異。5.在正常卵巢的顆粒黃體細(xì)胞及卵泡膜黃體細(xì)胞中均可觀察到GDF8及其受體蛋白的表達(dá),且在顆粒黃體細(xì)胞中表達(dá)顯著較高。6.在2-5mm及5-10mm竇卵泡的顆粒細(xì)胞/卵泡膜細(xì)胞中,GDF8及其受體蛋白在PCOS卵巢中表達(dá)顯著高于在正常卵巢的表達(dá);但是在0-2mm竇卵泡中,PCOS卵巢中的表達(dá)與正常卵巢中的表達(dá)無(wú)差異。結(jié)論GDF8及其相關(guān)功能受體在人類卵巢的生長(zhǎng)卵泡及黃體中的差異性表達(dá)提示它們可能在卵泡卵母細(xì)胞、顆粒細(xì)胞、卵泡膜細(xì)胞的生長(zhǎng)發(fā)育過(guò)程中起到調(diào)節(jié)作用。GDF8及其相關(guān)功能受體在PCOS卵巢和正常卵巢竇卵泡中的差異性表達(dá)提示其在PCOS發(fā)病機(jī)制中的潛在作用。
[Abstract]:Objective To investigate the expression of GDF8 and its related receptors in human normal ovary and PCOS ovarian follicles.Methods Immunohistochemistry was used to stain ovarian histopathological sections.The localization and expression intensity of GDF8 and its receptors in ovarian cells were evaluated by microscope.Results 1.GDF8 and its receptors (ACVR2A, ACVR2B, ALK5) were detected by immunohistochemistry. White was expressed in some granulosa cells of normal ovary and primary follicles of PCOS ovary, but not in granulosa cells of primordial follicles. 2. GDF8 and its receptors were expressed in oocytes of both normal ovary and growing follicles of PCOS ovary (from primordial follicles to 10 mm sinus follicles). The expression of GDF8 and its receptors in granulosa cells and follicular membrane cells of 0-2 mm, 2-5 mm, 5-10 mm sinus follicles in normal ovaries and PCOS ovaries increased significantly with the increase of the diameter of sinus follicles. 4. GDF8 and its receptors were expressed in granulosa cells of 2-5 mm and 5-1 Omm sinus follicles in normal ovaries and PCOS ovaries. The expression of GDF8 and its receptor protein in granulosa luteal cells and follicular membranous luteal cells of normal ovaries was significantly higher than that in granulosa luteal cells. 6. The expression of GDF8 and its receptor protein was significantly higher in granulosa luteal cells of 2-5mm and 5-10mm sinus follicles. In granulocyte/follicular membrane cells, the expression of GDF8 and its receptor protein in PCOS ovaries was significantly higher than that in normal ovaries, but in 0-2 mm sinus follicles, the expression of GDF8 and its receptor protein in PCOS ovaries was not different from that in normal ovaries. The differential expression of GDF8 and its related functional receptors in PCOS ovary and normal ovarian sinus follicles suggests its potential role in the pathogenesis of PCOS.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R711.75
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本文編號(hào)：2227080