【摘要】：目的觀察高糖高脂聯(lián)合低劑量STZ對版納微型豬骨骼肌肌肉素(Musclin)表達(dá)的影響。方法高糖高脂聯(lián)合低劑量STZ誘導(dǎo)版納微型豬T2DM模型,每月末測定血糖、血脂、胰島素及Musclin水平變化。12個月末處死動物,HE和PAS及骨骼肌糖原含量(M2酶標(biāo)儀,比色法)測定,ELISA檢測血清Musclin含量,Western blot檢測肌肉Musclin蛋白表達(dá)。結(jié)果喂養(yǎng)12月后,與對照(Con)組比較,模型(HSFD)組血糖[(4.75±0.2)vs(19.28±2.24)mmol/L]、TG[(0.82±0.14)vs(2.46±0.29)mmol/L]和TC[(2.29±0.35)vs(8.93±0.43)mmol/L]升高(P0.05),胰島素缺乏[(8.25±1.04)vs(3.81±0.39)mU/L,P0.05];骨骼肌萎縮,糖原合成減少[(0.42±0.05)vs(0.29±0.04)μg/ml,P0.05];血清Musclin達(dá)峰值[(249.56±19.14)vs(409.43±21.34)pg/ml,P0.05],骨骼肌組織Musclin蛋白表達(dá)升高21%(P0.05)。結(jié)論高糖高脂聯(lián)合STZ可誘導(dǎo)版納微型豬發(fā)生IR、糖尿病,促進(jìn)骨骼肌萎縮,抑制糖原合成,可能與信號分子Musclin激活有關(guān)。
[Abstract]:Objective to observe the effect of high glucose and high fat combined with low dose STZ on the expression of musculin (Musclin) in Banna miniature pigs. Methods Banna miniature pig T2DM model was induced by high glucose, high fat and low dose STZ. Blood glucose, blood lipid, insulin and Musclin levels were measured at the end of each month. The animals were killed at the end of 12 months, including HE, PAS and glycogen content in skeletal muscle (M2 enzyme marker). Serum Musclin content was detected by Elisa and the expression of Musclin protein in muscle was detected by Western blot. 緇撴灉鍠傚吇12鏈堝悗,涓庡鐓，

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