【摘要】：目的通過檢測(cè)系統(tǒng)性紅斑狼瘡(SLE)患者外周血單個(gè)核細(xì)胞(PBMCs)中自噬相關(guān)基因(Atgs)mTOR,Becline-1,LC3和p62的表達(dá)情況,分析其與SLE疾病活動(dòng)度(SLEDAI)及免疫學(xué)指標(biāo)的相關(guān)性,初步探討mTOR,Becline-1,LC3和p62在SLE發(fā)生發(fā)展中的作用。方法1.81例2015.10-2016.05就診于鄭州大學(xué)第一附屬醫(yī)院風(fēng)濕免疫科的SLE患者為實(shí)驗(yàn)組,采集所有實(shí)驗(yàn)對(duì)象的臨床、人口及實(shí)驗(yàn)室資料。同時(shí)期來(lái)自鄭州大學(xué)第一附屬醫(yī)院體檢科的健康志愿者86例為健康對(duì)照。清晨采集所有研究對(duì)象血標(biāo)本。2.RT-PCR檢測(cè)81例SLE患者及86例健康對(duì)照PBMCs中Atgs mTOR,Becline-1,LC3和p62的mRNA水平。分析SLE組和健康對(duì)照組間Atgs表達(dá)的差異及其與SLEDAI及免疫學(xué)指標(biāo)的相關(guān)性。3.統(tǒng)計(jì)學(xué)分析采用統(tǒng)計(jì)分析軟件SPSS21.0,均數(shù)±標(biāo)準(zhǔn)差((?)±S)與百分比(%)分別表示定量資料與定性資料。兩樣本間定量資料的比較采用t檢驗(yàn),多組定量資料的比較采用單因素方差分析或非參數(shù)檢驗(yàn)。定性資料比較采用χ2檢驗(yàn)。Person,s或Spearman秩相關(guān)分析用來(lái)評(píng)價(jià)Atgs mRNA水平與SLEDAI及免疫學(xué)指標(biāo)的相關(guān)性。P0.05被認(rèn)為有統(tǒng)計(jì)學(xué)意義。結(jié)果1.SLE組和健康對(duì)照組PBMCs中Atgs mRNA水平的比較:SLE組Becline-1,LC3和p62 mRNA水平均高于健康對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(Becline-1 mRNA:9.96×10~(-4) vs 7.38×10~(-4),P0.001;LC3 mRNA:4.04×10~(-5) vs 2.62×10~(-5),P0.001;p62 mRNA:9.51×10~(-4) vs 7.59×10~(-4),P=0.008);兩組間mTOR mRNA(2.97×10~(-4) vs 3.31×10~(-4),P=0.080)水平無(wú)顯著差異。2.SLE患者Becline-1 mRNA水平與SLEDAI及免疫學(xué)指標(biāo)的相關(guān)性:Becline-1 mRNA與SLEDAI(r=0.480;P0.00),IgG(r=0.511;P0.001)及抗ds-DNA抗體(r=0.620;P0.001)正相關(guān),與C3(r=-0.454;P0.001)負(fù)相關(guān),與C4(r=-0.180;P=0.108)無(wú)相關(guān)性。3.SLE患者LC3 mRNA水平與SLEDAI及免疫學(xué)指標(biāo)的相關(guān)性:LC3 mRNA與SLEDAI(r=0.350;P=0.001),IgG(r=0.594;P0.001)及抗ds-DNA抗體(r=0.439;P0.001)正相關(guān),與C3(r=-0.470;P0.001),C4(r=-0.251;P=0.024)負(fù)相關(guān)。4.SLE患者p62 mRNA水平與SLEDAI及免疫學(xué)指標(biāo)的相關(guān)性:p62 mRNA與SLEDAI(r=0.505;P0.001),IgG(r=0.542;P0.001)及抗ds-DNA抗體(r=0.631;P0.001)正相關(guān),與C3(r=-0.383;P0.001)負(fù)相關(guān),與C4(r=-0.215;P=0.054)無(wú)相關(guān)性。結(jié)論SLE患者PBMCs中存在自噬形成活躍與自噬降解受阻的現(xiàn)象,維持自噬水平的平衡可能有助于改善SLE疾病活動(dòng)及免疫學(xué)紊亂狀況。
[Abstract]:Objective to investigate the expression of autophagy associated genes (Atgs) mTORORline-1, LC3 and p62) in peripheral blood mononuclear cells (PBMC) of (SLE) patients with systemic lupus erythematosus (SLE), and to analyze their correlation with SLE disease activity (SLEDAI) and immunological parameters. To explore the role of mTORline-1 LC3 and p62 in the development of SLE. Methods 1.81 SLE patients in the Department of Rheumatology and Immunology, the first affiliated Hospital of Zhengzhou University, were selected as experimental group. The clinical, demographic and laboratory data of all subjects were collected. In the same period, 86 healthy volunteers from the Department of physical examination of the first affiliated Hospital of Zhengzhou University were healthy controls. The mRNA levels of Atgs mTORORline-1 LC3 and p62 in 81 patients with SLE and 86 healthy controls were detected by RT-PCR. To analyze the difference of Atgs expression between SLE group and healthy control group and its correlation with SLEDAI and immunological indexes. Statistical analysis software SPSS 21.0, mean 鹵standard deviation (?) 鹵S) and percentage (%) were used to represent quantitative and qualitative data respectively. T test was used to compare quantitative data between two samples, and single factor analysis of variance (ANOVA) or nonparametric test was used to compare multigroup quantitative data. Qualitative data were compared with 蠂 2 test. Persons or Spearman rank correlation analysis was used to evaluate the correlation between Atgs mRNA level and SLEDAI and immunological indexes. P05 was considered to have statistical significance. Results the levels of Atgs mRNA in PBMCs in 1.SLE group and healthy control group were higher than those in healthy control group. 宸紓鏈夌粺璁″鎰忎箟(Becline-1 mRNA:9.96脳10~(-4) vs 7.38脳10~(-4),P0.001;LC3 mRNA:4.04脳10~(-5) vs 2.62脳10~(-5),P0.001;p62 mRNA:9.51脳10~(-4) vs 7.59脳10~(-4),P=0.008);涓ょ粍闂磎TOR mRNA(2.97脳10~(-4) vs 3.31脳10~(-4),P=0.080)姘村鉤鏃犳樉钁楀樊寮，

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