【摘要】：文章利用高糖/高膽固醇飲食誘導(dǎo)了線蟲,油紅O染色結(jié)果表明線蟲脂肪沉積顯著增加。為了研究組織蛋白酶B在誘導(dǎo)線蟲過程中的作用,利用實時熒光定量聚合酶鏈式反應(yīng)(polymerase chain reaction,PCR)研究了組織蛋白酶B同源基因的表達。選取其中的關(guān)鍵基因利用RNA干擾(RNA interference,RNAi)技術(shù),研究其在線蟲脂肪沉積中的作用。結(jié)果表明:在線蟲脂肪沉積改變后,組織蛋白酶B同源基因表達隨之改變;而利用RNA干擾組織蛋白酶B關(guān)鍵基因cpr-3的表達,線蟲的脂肪沉積未受影響。因此,組織蛋白酶B同源基因可能參與線蟲脂肪沉積改變后的生理變化,而未直接參與線蟲脂肪沉積的調(diào)控。
[Abstract]:Nematode was induced by high sugar / high cholesterol diet. Oil red O staining showed that fat deposition of nematode increased significantly. In order to study the role of cathepsin B in the induction of nematodes, the expression of cathepsin B homologous genes was studied by real-time fluorescent quantitative polymerase chain reaction (polymerase chain). The key genes were selected to study the role of RNA interference (RNAi) in nematode fat deposition. The results showed that the expression of cathepsin B homologous genes changed after fat deposition, but the fat deposition of nematodes was not affected by interfering with the expression of cathepsin B key gene cpr-3. Therefore, cathepsin B homologous gene may be involved in the physiological changes of nematodes after fat deposition change, but not directly involved in the regulation of nematode fat deposition.
【作者單位】： 合肥工業(yè)大學(xué)生物與食品工程學(xué)院;
【基金】：安徽省自然科學(xué)基金資助項目(1408085QC48)
【分類號】：R589.2

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1 趙麗娟;葡萄糖誘導(dǎo)線蟲脂肪沉積的表觀遺傳學(xué)研究[D];合肥工業(yè)大學(xué);2015年

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