本文選題：對(duì)氧磷酶 + 敵敵畏中毒��； 參考：《中國(guó)醫(yī)科大學(xué)學(xué)報(bào)》2017年07期

【摘要】：目的探討純化兔血清對(duì)氧磷酶1(PON1)對(duì)敵敵畏中毒大鼠肝損傷的療效是否優(yōu)于傳統(tǒng)方法。方法將30只雄性SD大鼠隨機(jī)分為5組:空白對(duì)照組(A組)、敵敵畏染毒組(B組)、傳統(tǒng)治療組(C組)、PON1治療組(D組)、聯(lián)合治療組(E組),每組6只。B、C、D、E組均給予敵敵畏(9 mg/kg)腹腔注射染毒,C組在染毒后立即(2 min)給予阿托品(10 mg/kg)+碘解磷定(45mg/kg)腹腔注射,D組在染毒前30 min給予PON1 9 600 U/kg尾靜脈注射,E組染毒前30 min給予PON1 9 600 U/kg尾靜脈注射,染毒后立即(2 min)給予阿托品(10 mg/kg)+碘解磷定(45 mg/kg)腹腔注射,A組給予等量的生理鹽水對(duì)照。各組于造模前(0min)、造模后10 min、30 min、1 h、2 h、4 h、6 h分別頸內(nèi)動(dòng)脈取血,運(yùn)用ELISA方法檢測(cè)血清乙酰膽堿酯酶活性。各組分別于造模后12 h取肝臟組織,HE染色觀察不同組肝臟的病理改變,運(yùn)用免疫組化及Western blotting檢測(cè)不同組肝臟4-羥基壬烯醛(4-HNE)的定性及定量表達(dá)。結(jié)果 B組膽堿酯酶明顯下降,光鏡下出現(xiàn)肝細(xì)胞嚴(yán)重脂肪變、核固縮等病理改變,可見(jiàn)4-HNE高度表達(dá);C、D、E組上述各指標(biāo)改變均較B組輕,且各組與B組之間均存在統(tǒng)計(jì)學(xué)差異(P0.05);D、E組病理改變較C組輕,上述指標(biāo)與C組之間均有統(tǒng)計(jì)學(xué)差異(P0.05);E組較D組病理改變稍輕,上述指標(biāo)無(wú)統(tǒng)計(jì)學(xué)差異(P0.05)。結(jié)論 PON1對(duì)急性敵敵畏中毒大鼠肝損傷發(fā)揮保護(hù)作用,且效果優(yōu)于傳統(tǒng)治療。
[Abstract]:Objective to investigate the effect of purified rabbit serum paraoxonase 1 (PON1) on liver injury in dichlorvos poisoned rats. Methods Thirty male Sprague-Dawley rats were randomly divided into 5 groups: blank control group (group A), dichlorvos exposure group (group B), traditional treatment group (group C) treated with PON1 (group D), combined treatment group (group E), each group of 6 rats were given dichlorvos (9 mg/kg). Group C was given atropine (10 mg/kg) iodifloxide (45mg/kg) immediately (2 min) by intraperitoneal injection, and group D was given PON1 9 600 U / kg caudal vein 30 min before exposure, and PON1 9 600 U / kg caudal vein was injected 30 min before exposure. Immediately after exposure (2 min), atropine (10 mg/kg) was injected intraperitoneally with atropine (10 mg/kg) and iodide (45 mg/kg). Group A was given the same amount of normal saline as control group. Serum acetylcholinesterase activity was detected by Elisa in each group before modeling (0min) and 10 min 30 min, 1 h, 2 h, 4 h and 6 h, respectively. The pathological changes of liver in different groups were observed by HE staining at 12 h after modeling. The qualitative and quantitative expression of 4-hydroxynoneneal (4-HNE) in different groups was detected by immunohistochemistry and Western blotting. Results the changes of cholinesterase in group B were significantly lower than those in group B (P < 0.01). The pathological changes such as severe fatty degeneration and nuclear pyknosis in liver cells were observed under light microscope. The changes of the above indexes in group 4-HNE were lighter than those in group B. And there was statistical difference between each group and group B (P0.05). The pathological changes in group E were lighter than those in group C (P0.05). The pathological changes in group E were slightly lighter than those in group D (P0.05). There was no statistical difference in the above indexes (P0.05). Conclusion PON1 has a protective effect on liver injury in rats with acute dichlorvos poisoning, and the effect is better than that of traditional treatment.
【作者單位】： 中國(guó)醫(yī)科大學(xué)附屬盛京醫(yī)院急診科;
【基金】：國(guó)家自然科學(xué)基金(81671898) 盛京自由研究者計(jì)劃(201207)
【分類號(hào)】：R595.4

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