本文選題：肥胖 + n-3多不飽和脂肪酸��； 參考：《青島大學(xué)》2015年碩士論文

【摘要】：目的研究fat-1轉(zhuǎn)基因小鼠增加內(nèi)源性n-3多不飽和脂肪酸(Polyunsaturated fat acids,PUFAs)對小鼠肥胖的抵抗作用,從體重、血清中血脂及胃組織中肥胖相關(guān)因子nesfatin-1和ghrelin的變化,探討其作用機理。方法將小鼠分為高脂飲食fat-1轉(zhuǎn)基因組和非轉(zhuǎn)基因組及正常飲食fat-1轉(zhuǎn)基因組和非轉(zhuǎn)基因組4個組。每周測量各組小鼠體質(zhì)量和體長,實驗結(jié)束時處死四組小鼠,稱量小鼠內(nèi)臟周圍脂肪質(zhì)量;取血測定各組小鼠血清中血糖(FBG)、甘油三酯(TG)、總膽固醇(TC)、高密度脂蛋白膽固醇(HDL-C)、低密度脂蛋白膽固醇(LDL-C)含量;測定胃組織內(nèi)nesfatin-1、ghrelin m RNA的相對表達量和蛋白表達量及其與Lee's指數(shù)的相關(guān)性。結(jié)果轉(zhuǎn)基因小鼠的內(nèi)臟周圍脂肪質(zhì)量均低于非轉(zhuǎn)基因小鼠(F=20.56~41.75,P0.01);轉(zhuǎn)基因小鼠血清FBG、TG、TC、HDL-C、LDL-C水平均低于非轉(zhuǎn)基因小鼠(F=15.59~94.07,P0.01);高脂飲食使nesfatin-1 m RNA的相對表達量顯著上調(diào)(F=25.75,P0.01),并且與Lee's指數(shù)成正相關(guān)(r=0.621,P0.01),轉(zhuǎn)基因小鼠比非轉(zhuǎn)基因小鼠表達量低并且差異顯著(q=7.035,6.012,P0.05)。高脂飲食使ghrelin m RNA的相對表達量顯著下調(diào)(F=28.42,P0.01),且與Lee's指數(shù)成負相關(guān)(r=-0.603,P0.01),轉(zhuǎn)基因小鼠均比非轉(zhuǎn)基因小鼠表達量高,并且差異具有統(tǒng)計學(xué)意義(q=4.112,3.379,P0.05)。結(jié)論Fat-1轉(zhuǎn)基因小鼠體內(nèi)含有的n-3多不飽和脂肪酸脫氫酶增加了內(nèi)源性n-3PUFA,從而降低體內(nèi)n-6/n-3比值,降低了小鼠體重,調(diào)節(jié)了血脂及肥胖因子的表達,改善機體能量代謝,預(yù)防了肥胖的發(fā)生。
[Abstract]:Objective to study the effects of increasing endogenous n-3 fat polyunsaturated fatty acids (PUFAs) on obesity in fat-1 transgenic mice, and to explore its mechanism from the changes of body weight, serum lipids and nesfatin-1 and ghrelin in gastric tissues. Methods mice were divided into four groups: high fat diet fat-1 transgenic group and non-transgenic group, normal diet fat-1 transgenic group and non-transgenic group. The body weight and body length of each group were measured every week. At the end of the experiment, the four groups of mice were killed to weigh the weight of fat around the viscera of the mice. Serum glucose (FBG), triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were measured. The relative expression and protein expression of nesfatin-1 hghrelin mRNA in gastric tissue and its correlation with Leehs index were measured. Results the quality of visceral fat in transgenic mice was significantly lower than that in non-transgenic mice (FB20.56A 41.75U P0.01), the serum levels of FBGG + TGG + TGG (HDL-CU LDL-C) in transgenic mice were lower than those in non-transgenic mice (F15.5994.07g / P0.01), and the relative expression of nesfatin-1 mRNA was significantly up-regulated by high-fat diet (FG 25.75U P0.01), and the relative expression of nesfatin-1 mRNA was significantly higher than that of non-transgenic mice (FF25.75P0.01), and the level of LDL-C in serum of transgenic mice was lower than that of non-transgenic mice. The expression level of transgenic mice was significantly lower than that of non-transgenic mice (P 0.05). The relative expression of ghrelin mRNA was significantly down-regulated by high-fat diet (F _ (28.42) P _ (0.01), and negatively correlated with the Leehs index (r ~ (-0.603) P _ (0.01). The expression of ghrelin mRNA in transgenic mice was significantly higher than that in non-transgenic mice (P < 0.05). Conclusion n-3 polyunsaturated fatty acid dehydrogenase in Fat-1 transgenic mice can increase endogenous n-3PUFAs, decrease the ratio of n-6/n-3 in vivo, decrease the body weight, regulate the expression of lipid and obesity factors, and improve energy metabolism. To prevent obesity.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R589.2

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中國期刊全文數(shù)據(jù)庫 前1條

1 徐姝迪;鄭玉建;丁紅;姬鳳彩;王忠;艾力·吾布里卡斯木;任美玲;;高脂飼料誘導(dǎo)肥胖大鼠模型的方法研究[J];現(xiàn)代預(yù)防醫(yī)學(xué);2012年02期

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本文編號：2105893