發(fā)布時(shí)間：2018-07-03 20:25

  本文選題：海拔 + 超氧化歧化酶　； 參考：《青海大學(xué)》2015年碩士論文

【摘要】：目的:通過建立三個(gè)不同海拔的動(dòng)物實(shí)驗(yàn)?zāi)Ｐ?研究對(duì)照大鼠血漿中超氧化物歧化酶、丙二醛水平的變化,旨在探討不同海拔對(duì)大鼠血漿中超氧化歧化酶活性、丙二醛含量變化的影響,為高海拔低氧環(huán)境下急慢性機(jī)體損傷的防治提供重要的理論基礎(chǔ)。方法:24只雄性大鼠隨機(jī)分為對(duì)照組(蘭州,海拔1500米)、中度海拔組(西寧,海拔2260米)和高海拔組(低壓氧艙,模擬海拔5000米)。將中度海拔組和高海拔組大鼠分別由蘭州快速運(yùn)至西寧地區(qū)和青海大學(xué)低壓氧艙室,30天后抽取大鼠下腔靜脈血標(biāo)本,分別用水溶性四氮唑法測(cè)定超氧化歧化酶活力,硫代巴比妥酸比色法測(cè)定丙二醛含量。結(jié)果 :對(duì)照組大鼠血漿中超氧化歧化酶、丙二醛水平分別為(19.98±1.57)、(2.28±0.68),中海拔組血漿中超氧化歧化酶、丙二醛水平分別為(19.06±1.24)、(2.76±0.34),高海拔組大鼠血漿中超氧化歧化酶、丙二醛水平分別為(10.34±1.19)、(6.35±0.58)。高海拔組大鼠血漿中超氧化歧化酶活力明顯高于中海拔組及對(duì)照組,丙二醛含量明顯高于中海拔組及對(duì)照組(均P0.001);中海拔組大鼠血漿中超氧化歧化酶活力略低于對(duì)照組,高于模擬特高海拔組,丙二醛含量略高于對(duì)照組,低于模擬特高海拔組(均P0.001)。結(jié)論:在高海拔低氧條件下,大鼠血漿中超氧化歧化酶活力逐漸下降、丙二醛含量逐漸增加,提示機(jī)體抗氧化損傷能力減弱、機(jī)體細(xì)胞氧化損傷進(jìn)行性發(fā)展、組織中自由基的含量進(jìn)行性升高、由其導(dǎo)致的脂質(zhì)過氧化反應(yīng)程度越來越嚴(yán)重、機(jī)體細(xì)胞損傷的程度越來越嚴(yán)重。
[Abstract]:Objective: to study the changes of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in plasma of control rats by establishing three animal models at different elevations in order to study the activity of superoxide dismutase (SOD) in plasma of rats at different elevations. The effect of malondialdehyde content provides an important theoretical basis for the prevention and treatment of acute and chronic organism injury in high altitude and low oxygen environment. Methods Twenty four male rats were randomly divided into control group (Lanzhou, 1500 meters above sea level), moderate altitude group (Xining, 2260 meters above sea level) and high altitude group (low oxygen chamber, simulated altitude 5000 meters). The blood samples of inferior vena cava of rats in moderate altitude group and high altitude group were collected after 30 days of rapid transport from Lanzhou to Xining area and low pressure oxygen chamber of Qinghai University. The activity of superoxide dismutase (SOD) was determined by water-soluble tetrazolium method. The content of malondialdehyde was determined by thiobarbituric acid colorimetry. Results: the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were (19.98 鹵1.57), (2.28 鹵0.68) in the control group, (19.06 鹵1.24), (鹵0.34) in the middle altitude group, and (10.34 鹵1.19), (/ 6.35 鹵0.58) in the high altitude group. The superoxide dismutase activity in plasma of rats in high altitude group was significantly higher than that in middle altitude group and control group, and the content of malondialdehyde in plasma was significantly higher than that in middle altitude group and control group (P0.001), and the activity of superoxide dismutase in plasma of middle altitude group was slightly lower than that of control group. The content of malondialdehyde was slightly higher than that of the control group and lower than that of the simulated super high altitude group (all P0.001). Conclusion: under the condition of high altitude hypoxia, the activity of superoxide dismutase (SOD) in plasma and the content of malondialdehyde (MDA) in plasma of rats were gradually decreased, which indicated that the ability of antioxidant damage was weakened and the oxidative damage of cells was gradually developed. The content of free radicals in tissues is increasing, the degree of lipid peroxidation is becoming more and more serious, and the degree of cell damage is becoming more and more serious.
【學(xué)位授予單位】：青海大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R594.3

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