本文選題：干擾素 + OAS1　； 參考：《遵義醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:通過檢測系統(tǒng)性紅斑狼瘡(Systemic lupus erythematosus,SLE)患者及健康對照組外周血中IFN-α、IFN-β及OAS1的水平,初步探討SLE患者IFN-α、IFN-β及OAS1的水平與SLE的相關(guān)性。方法:分別收集30例SLE患者、30例健康對照者外周靜脈血,離心分離血漿,ELISA法分別檢測兩組IFN-α、IFN-β及OAS1蛋白的水平;分析IFN-α及IFN-β與OAS1之間的相關(guān)性;分析血漿中IFN-α、IFN-β及OAS1蛋白水平與系統(tǒng)性紅斑狼瘡疾病活動性指數(shù)(systemic lupus erythematosus disease activity index,SLEDAI)、SLE患者的實驗室檢測指標(biāo)及臨床癥狀的相關(guān)性。結(jié)果:(1)IFN-α、IFN-β及OAS1蛋白的ELISA檢測結(jié)果:SLE患者組IFN-α、IFN-β及OAS1的水平均較健康對照組顯著增氋(369.56±127.69pg/ml vs.171.05±69.43pg/ml,P(27)0.05;1488.78±364.73pg/ml vs.990.22±348.76pg/ml,P(27)0.05;10.87±2.23ng/ml vs.2.27±0.25ng/ml,P(27)0.05)。(2)SLE患者組IFN-α及IFN-β與OAS1的相關(guān)性分析結(jié)果:IFN-α的水平與OAS1正相關(guān)(r=0.439,P=0.015),IFN-β的水平與OAS1無相關(guān)性(r=-0.038,P=0.844)。(3)SLE患者組IFN-α、IFN-β及OAS1與SLEDAI的相關(guān)性分析結(jié)果:IFN-α、OAS1的水平與SLEDAI評分正相關(guān)(r=0.534,P=0.002;r=0.505,P=0.004),IFN-β的水平與SLEDAI評分無相關(guān)性(r=-0.306,P=0.100)。(4)SLE患者組IFN-α、IFN-β及OAS1與實驗室檢測指標(biāo)的相關(guān)性分析結(jié)果:IFN-α的水平與抗核抗體(ANA)和抗雙鏈DNA抗體(抗ds-DNA抗體)滴度正相關(guān)(r=0.7000,P=0.000;r=0.468,P=0.016),與抗Sm抗體等自身抗體無相關(guān)(P0.05),血沉升高、補體C3下降陽性組較陰性組IFN-α的水平有顯著差異(Z=-2.915,P=0.003;Z=-2.173,P=0.03),白細胞減少等指標(biāo)陽性組較陰性組IFN-α的水平無差異(P0.05);IFN-β的水平與抗核抗體和自身抗體無相關(guān)性(P0.05),且在實驗室檢測指標(biāo)陽性組較陰性組的差異無統(tǒng)計學(xué)意義(P0.05);OAS1的水平與ANA和抗ds-DNA抗體滴度正相關(guān)(r=0.374,P=0.042;r=0.424,P=0.031),OAS1的水平與抗Sm抗體等自身抗體無相關(guān)性(P0.05),且在實驗室檢測指標(biāo)陽性組和陰性組的差異無統(tǒng)計學(xué)意義(P0.05)。(5)SLE患者組IFN-α、IFN-β及OAS1與臨床癥狀的相關(guān)性分析結(jié)果:腎損害與光過敏陽性組較陰性組IFN-α的水平有差異(Z=-2.318,P=0.020;Z=-2.928,P=0.003),關(guān)節(jié)炎、發(fā)熱及顴狀紅斑等臨床癥狀陽性組較陰性組IFN-α的水平均無差異;腎損害、關(guān)節(jié)炎等臨床癥狀陽性組較陰性組IFN-β、OAS1的水平均無差異(P0.05)。結(jié)論:(1)SLE患者組血漿中IFN-α、IFN-β及OAS1水平較正常組增高,且IFN-α與OAS1的水平正相關(guān),而IFN-β與OAS1水平無相關(guān)性,提示OAS1可能受IFN-α的調(diào)節(jié)從而在SLE發(fā)病中發(fā)揮作用。(2)SLE組IFN-α和OAS1的水平與SLEDAI評分、ANA及抗ds-DNA抗體滴度正相關(guān),IFN-α的水平與血沉升高、補體C3下降、腎損害、光過敏有關(guān),而IFN-β、OAS1水平與實驗室檢測指標(biāo)及臨床癥狀均無關(guān),提示IFN-α及OAS1可能與SLE病情活動性有關(guān),且IFN-α可能參與SLE的病理過程。
[Abstract]:Objective: to investigate the correlation between the levels of IFN- 偽, IFN- 尾 and OAS1 in peripheral blood of patients with systemic lupus erythematosus (SLE) and healthy controls by detecting the levels of IFN- 偽 -IFN- 尾 and OAS1 in peripheral blood of patients with systemic lupus erythematosus (SLE). Methods: peripheral venous blood was collected from 30 patients with SLE and 30 healthy controls. The plasma levels of IFN- 偽 -IFN- 尾 and OAS1 were detected by centrifugation Elisa, and the correlation between IFN- 偽, IFN- 尾 and OAS1 was analyzed. To analyze the correlation between the levels of IFN- 偽 -IFN- 尾 and OAS1 protein and the laboratory indexes and clinical symptoms of SLE patients with systemic lupus erythematosus disease activity index (systemic lupus erythematosus disease activity). 緇撴灉:(1)IFN-偽,IFN-尾鍙奜AS1铔嬬櫧鐨凟LISA媯，

本文編號：2083028