本文選題：姜黃素 + 干熱環(huán)境　； 參考：《石河子大學(xué)》2017年碩士論文

【摘要】：目的:通過姜黃素預(yù)處理大鼠并復(fù)制干熱環(huán)境下大鼠中暑模型,探討姜黃素對干熱環(huán)境下大鼠腎損傷的保護(hù)作用及其可能機(jī)制。方法:成年SD雄性大鼠200只,隨機(jī)數(shù)字表法分為四:0分鐘組(常溫組)、50分鐘組、100分鐘組、150分鐘組。每組下分為五個(gè)亞組(n=10):鹽水(NS)組、溶劑(CMCNa)組、姜黃素50mg/kg組(Cur 50mg/kg)、姜黃素100mg/kg組(Cur 100mg/kg)、姜黃素200mg/kg組(Cur 200mg/kg)。鹽水組給予0.9%生理鹽水灌胃,溶劑組給予0.5%CMCNa溶液灌胃,姜黃素組大鼠給予不同濃度姜黃素溶液灌胃,連續(xù)七天,每日一次。復(fù)制干熱環(huán)境下大鼠中暑模型:將大鼠置于模擬沙漠干熱環(huán)境中(西北特殊環(huán)境人工實(shí)驗(yàn)艙,中國人民解放軍烏魯木齊總醫(yī)院研制),設(shè)置溫度41±0.5℃,濕度10±1%,每25分鐘監(jiān)測大鼠核心體溫。根據(jù)分組時(shí)間取出大鼠,進(jìn)行麻醉,腹主動(dòng)脈采血進(jìn)行生化檢測,穿刺膀胱留取尿液進(jìn)行腎損傷分子-1(KIM-1)及中性粒細(xì)胞明膠酶相關(guān)載脂蛋白(NGAL)的檢測,取腎組織用于病理及分子生物學(xué)檢測。結(jié)果:(1)在50min、100min及150min組,不同濃度的姜黃素處理后的大鼠血清中尿素、肌酐、KIM-1及NGAL的含量較同一時(shí)間點(diǎn)的鹽水(NS)組和溶劑組(CMCNa)明顯下降(P0.05)。(2)在干熱環(huán)境中,不同濃度的姜黃素可降低腎組織中MDA含量及提高SOD和CAT的活性,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。(3)在同一時(shí)間點(diǎn)干熱環(huán)境下,姜黃素預(yù)處理組大鼠腎組織內(nèi)IL-1β、IL-6和TNF-α的水平較鹽水(NS)組及溶劑(CMCNa)組降低,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。(4)在150min組,不同濃度的姜黃素預(yù)處理可明顯改善大鼠腎組織的病理改變。(5)大鼠腎組織石蠟切片TUNEL染色顯示,在150min組,姜黃素預(yù)處理可降低腎臟細(xì)胞的凋亡率,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。(6)在150min組,姜黃素預(yù)處理可降低腎組織內(nèi)炎癥反應(yīng)相關(guān)蛋白及凋亡相關(guān)蛋白的表達(dá)水平,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:(1)持續(xù)的干熱環(huán)境對大鼠腎臟具有明顯的損傷作用,并且隨著持續(xù)時(shí)間的延長而加重,腎組織內(nèi)炎癥反應(yīng)及氧化應(yīng)激水平升高,腎組織細(xì)胞凋亡顯著。(2)干熱環(huán)境下,不同濃度的姜黃素均可不同程度的減輕大鼠腎損傷,其中200mg/kg時(shí)效果最顯著。(3)姜黃素可能通過抑制炎癥及氧化應(yīng)激反應(yīng)、阻斷細(xì)胞凋亡通路,在一定程度上減輕了干熱環(huán)境下大鼠腎損傷。
[Abstract]:Aim: to investigate the protective effect of curcumin on renal injury and its possible mechanism by pretreating rats with curcumin and making heat stroke model in dry heat environment. Methods: 200 adult Sprague-Dawley male rats were randomly divided into 4: 0 minute group (normal temperature group), 50 minutes group, 100 minutes group and 150 minutes group. Each group was divided into five subgroups: saline group (NS), solvent group (CMCNa), curcumin 50mg/kg group (curcumin 50mg/kg), curcumin 100mg/kg group (curcumin 100mg/kg), curcumin 200mg/kg group (curcumin 200mg/kg). The saline group was given 0.9% normal saline, the solvent group was given 0.5 CMCNa solution, and the curcumin group was given curcumin solution of different concentrations once a day for seven days. Rat model of heatstroke in dry and hot environment: rats were placed in a simulated desert dry and hot environment (artificial laboratory module for special environment in northwest China, developed by Urumqi General Hospital of Chinese people's Liberation Army), setting temperature was 41 鹵0.5 鈩，

本文編號(hào)：2077970