本文選題：CC趨化因子受體 + 拮抗短肽�。� 參考：《中國病理生理雜志》2017年04期

【摘要】：目的:研究與CC趨化因子受體5(CC chemokine receptor 5,CCR5)第二胞外環(huán)特異性結(jié)合的拮抗短肽對(duì)哮喘小鼠肺組織炎癥細(xì)胞浸潤和TNF-α表達(dá)的影響。方法:篩選最適宜的卵白蛋白(ovalbumin,OVA)致敏濃度,構(gòu)建OVA誘導(dǎo)的BALB/c小鼠哮喘模型。模型構(gòu)建成功后通過尾靜脈注射不同濃度拮抗短肽干預(yù)模型小鼠,HE染色對(duì)肺組織進(jìn)行病理細(xì)胞學(xué)分析及炎癥分級(jí),real-time PCR與Western blot實(shí)驗(yàn)分別檢測小鼠肺組織TNF-α的mRNA和蛋白表達(dá)水平。結(jié)果:篩選出的OVA最佳致敏濃度為500 mg/L(0.1 mL)。尾靜脈注射0.2m L不同濃度(1.5 g/L、2.5 g/L和3.5 g/L)拮抗短肽干預(yù)哮喘小鼠,能減輕哮喘小鼠肺組織炎癥程度,抑制TNF-α的表達(dá),以2.5 g/L濃度效果最佳,較模型組炎癥程度減輕2級(jí),炎癥細(xì)胞數(shù)明顯減少,TNF-α的mRNA表達(dá)量較模型組下降近90%,蛋白表達(dá)水平下降約70%。同時(shí)該短肽(2.5 g/L)也起到了一定的預(yù)防作用,炎癥程度改善1級(jí),TNF-α的mRNA及蛋白水平較模型組下降約50%左右。結(jié)論:CCR5第二胞外環(huán)的拮抗短肽能有效減輕哮喘小鼠肺組織炎癥程度,抑制TNF-α的表達(dá)。
[Abstract]:Aim: to study the effects of antagonistic short peptide binding to the second extracellular ring of CC chemokine receptor 5 (CC chemokine receptor 5) on inflammatory cell infiltration and expression of TNF- 偽 in lung tissue of asthmatic mice. Methods: the optimal concentration of ovalbumin OVA was selected to construct the BALB / c mouse asthma model induced by OVA. After the successful construction of the model, the lung tissue was stained by HE staining with different concentrations of antagonistic short peptide. The expression of TNF- 偽 mRNA and protein in the lung tissue was detected by real-time PCR and Western blot respectively. Results: the best sensitizing concentration of OVA was 500 mg / L (0.1 mL). Different concentrations of 0.2m L (1.5 g / L, 2.5 g / L and 3.5 g / L) antagonized short peptide in asthma mice, which could reduce the degree of lung inflammation and inhibit the expression of TNF- 偽 in asthmatic mice. The concentration of 2.5 g / L was the best, and the degree of inflammation in model group was reduced by 2 grades. Compared with the model group, the expression of TNF- 偽 mRNA in the inflammatory cells decreased by 90%, and the protein expression level decreased about 70%. At the same time, the short peptide (2.5 g / L) also played a preventive role. The level of TNF- 偽 mRNA and protein decreased by about 50% compared with the model group. Conclusion the antagonistic peptide of the second extracellular ring of 1: CCR5 can effectively reduce the degree of lung inflammation and inhibit the expression of TNF- 偽 in asthmatic mice.
【作者單位】： 中山大學(xué)孫逸仙紀(jì)念醫(yī)院兒科;中山大學(xué)孫逸仙紀(jì)念醫(yī)院病理科;
【基金】：廣東省自然科學(xué)基金資助項(xiàng)目(No.2014A030313020;No.2015A030313027;No.2016A03031343)
【分類號(hào)】：R562.25

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