本文選題：咖啡豆提取物 + 脂聯(lián)素　； 參考：《浙江工業(yè)大學》2015年碩士論文

【摘要】：目的:比較兩種咖啡豆提取物(CBE(一)和CBE(二))對營養(yǎng)肥胖模型大鼠的減肥作用,并對其中CBE(一)的減肥的作用機制進行了初步研究,為臨床應用作前期基礎(chǔ)研究。方法:按“國食藥監(jiān)�；痆2012]107號”文附件8減肥功能評價方法進行造模并給藥,觀察給藥組與模型對照組(MC)的情況,記錄CBE(一)和CBE(二)各組大鼠的體重變化、攝食量;試驗結(jié)束,測定各組大鼠體內(nèi)脂肪重量、全自動生化儀測定CBE(一)血清中總膽固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)的值;用Elisa法檢測脂聯(lián)素(APN)、瘦素(LP);試劑盒檢測游離脂肪酸(NEFA)的含量;用Western blot法檢測各組大鼠肝臟組織AdipoR2、p38、PGC-1、PPARγ蛋白的表達和脂肪組織中AdipoR2、PGC-1、PPARγ蛋白的表達以及肌肉組織中AdipoR2、PGC-1的表達;用RT-PCR法測定大鼠肝臟組織中AdipoR2、p38、PPARγ、SREBF1、SREBF2基因表達水平。結(jié)果:大鼠營養(yǎng)肥胖模型成功建立,兩種咖啡豆提取物均有減肥作用。具體表現(xiàn)在:與正常對照組(NC)大鼠比較,模型對照組(MC)的體重終重、體重增重和脂體比有明顯差異(P0.01);與MC比較,兩種給藥組大鼠體重終重、體重增重和脂體比均下降,均數(shù)間差異均有顯著性(P0.01)。與NC比較,MC大鼠TC、TG、LDL均升高,HDL含量下降,差異有顯著性(P0.01);與MC比較,CBE(一)(133mg·kg-1、267 mg·kg-1、800 mg·kg-1)三個劑量組TC、TG均降低,HDL均升高,其中133 mg·kg-1、267 mg·kg-1兩個劑量組LDL無明顯變化,而800 mg·kg-1組LDL降低(P0.05)。同時與NC相比,MC大鼠APN均明顯降低(P0.01),LP增多;與MC相比,CBE(一)給藥組大鼠APN增多(P0.01或0.05),LP降低。Western blot實驗表明,與MC相比,CBE(一)給藥組大鼠肝臟組織中AdipoR2、p38、PGC-1、PPARγ蛋白表達增多,脂肪組織中AdipoR2、PGC-1、PPARγ蛋白表達增多,而肌肉組織中AdipoR2、PGC-1蛋白表達增多,未檢測出PPARγ蛋白的表達。RT-PCR結(jié)果表明,大鼠肝臟SREBF1、SREBF2 mRNA顯著降低,而AdipoR2、p38、PPARγmRNA顯著增多。結(jié)論:按“國食藥監(jiān)�；痆2012]107號”文附件8減肥功能評價方法進行試驗,兩種咖啡豆提取物均有減肥作用,其中其作用機制可能是通過升高血清APN水平,降低LP水平,PGC-1表達增加,在一定程度上上調(diào)PPARγ的水平,從而促進脂肪組織釋放APN,增加APN與Adipo R2結(jié)合激活下游的AMPKα、p38、PPARα,下調(diào)SREBF1,從而促進脂肪的分解,抑制脂肪的生成,達到減肥的效果。
[Abstract]:Objective: to compare the effects of two kinds of coffee bean extracts (CBE (1) and CBE (2) on the weight loss of nutritional obese rats. Methods: according to the evaluation method of weight-loss function in annex 8 of "National Food and Drug Administration Supervision, Baohua [2012] 107", the changes of body weight and food intake were observed between the administration group and the model control group (MC), and the changes of body weight and the food intake were recorded in each group (CBE (1) and CBE (2). Total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL) in the serum of CBE (I) were measured by automatic biochemical instrument. Adiponectin (APN), leptin (LP), free fatty acid (NEFA) were detected by Elisa, AdipoR2P38-PGC-1PPAR 緯 protein and AdipoR2PGC-1PPAR 緯 protein were detected by Western blot assay, and AdipoR2PGC-1PPAR 緯 protein in muscle tissue were detected by Western blot assay. The expression of AdipoR2 (p38) PPAR 緯 -SREBF1 (SREBF2) gene in rat liver was determined by RT-PCR. Results: the model of nutritional obesity in rats was established successfully. Compared with the normal control group (NC), the model control group (MC) had a significant difference in body weight, weight gain and fat body ratio (P0.01), and compared with MC, the weight gain, weight gain and lipids weight ratio of the two groups were decreased, and compared with the control group, the weight gain and fat body weight ratio of the model control group (MC) were significantly lower than that of the normal control group (P0.01). There were significant differences between the mean values (P0.01). Compared with NC, TCU TGG-LDL increased and decreased significantly (P0.01), compared with MC (1) (133mg kg-1267 mg kg-1800 mg kg-1), TCG-TG decreased significantly, and 133mg kg-1267 mg kg-1 had no significant change, but 800mg kg-1 decreased LDL-C (P0.05). At the same time, compared with NC, APNs of MC rats decreased significantly (P0.01) and those of MC-treated rats decreased. Western blot assay showed that compared with MC group, the expression of AdipoR2p38PGC-1PPAR 緯 protein in liver tissue of rats treated with MC-CBE (I) was higher than that of MC group. The expression of PPAR 緯 protein in adipose tissue was higher than that in muscle tissue. The expression of PPAR 緯 protein was not detected in adipose tissue. The results of RT-PCR showed that the expression of PPAR 緯 protein in rat liver was significantly decreased, while that of AdipoR2p38PPAR 緯 mRNA was significantly increased in rat liver. Conclusion: according to the evaluation method of weight-loss function in Annex 8 of "National Food and Drug Supervision, Baohua [2012] 107", the two kinds of extracts of coffee bean have the effect of reducing weight loss, and the mechanism may be to increase the level of serum APN and increase the expression of PGC-1 by increasing serum APN level, reducing the level of LP and increasing the expression of PGC-1. The level of PPAR- 緯 was upregulated to a certain extent, which promoted the adipose tissue to release APN, increased APN binding with Adipo R2 and activated the downstream AMPK 偽 p38 PPAR 偽, down-regulated SREBF1, thus promoted the decomposition of fat, inhibited the formation of fat, and achieved the effect of weight loss.
【學位授予單位】：浙江工業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R589.2

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