本文選題：HIV + CD淋巴細(xì)胞�。� 參考：《現(xiàn)代預(yù)防醫(yī)學(xué)》2017年18期

【摘要】：目的評價(jià)美國Beckman Coulter公司(BC公司)的三色熒光抗體干粉試劑(CD3/CD4/CD8)在流式細(xì)胞儀上檢測CD4細(xì)胞計(jì)數(shù)及CD4細(xì)胞百分比的準(zhǔn)確性。方法于2015年12月開始在云南省疾病預(yù)防控制中心收集艾滋病自愿咨詢檢測(VCT)門診的血液樣本和送檢樣本共計(jì)201份,分別用美國的Becton Dickinson公司(BD公司)的液態(tài)三色熒光抗體試劑和BC公司的干粉試劑進(jìn)行樣本處理,使用BD FACSCalibur流式細(xì)胞儀和Multiset軟件程序進(jìn)行CD4細(xì)胞絕對計(jì)數(shù)和百分比分析,用SPSS軟件和Bland-Altman圖對2種試劑檢測結(jié)果的一致性進(jìn)行分析。結(jié)果 BC和BD試劑對201例樣本的CD4細(xì)胞計(jì)數(shù)的均值分別為(506.3±605.3)個(gè)/μl(10~2 458)和(501.0±610.3)個(gè)/μl(7~2 406),Pearson相關(guān)系數(shù)為0.984,平均偏差為+5.3個(gè)/μl(95%CI:-102.3~113.0);CD4細(xì)胞百分比的結(jié)果具有很好的相關(guān)性,Pearson相關(guān)系數(shù)為0.992,平均偏差為+0.66%(95%CI:-3.6%~4.9%)。以CD4細(xì)胞計(jì)數(shù)等于350個(gè)/μl為治療閾值,BC干粉試劑的敏感性和特異性分別為95.8%和98.4%。結(jié)論與BD試劑相比,BC干粉試劑在FACSCalibur流式細(xì)胞儀上檢測CD4細(xì)胞的絕對數(shù)和百分比具有較好的相關(guān)性和一致性,干粉試劑的保存和運(yùn)輸無需冷鏈,適合基層單位推廣應(yīng)用。
[Abstract]:Objective to evaluate the accuracy of Beckman Coulter (BC) tricolor fluorescent antibody dry powder reagent (CD3 / CD4 / CD8) in detecting CD4 cell count and percentage of CD4 cells by flow cytometry. Methods in December 2015, 201 blood samples were collected from the outpatient clinic of Voluntary Counseling and testing (VCT) for AIDS at the Centers for Disease Control and Prevention of Yunnan Province. The samples were treated with Becton Dickinson Inc. (BD) liquid tricolor fluorescent antibody reagent and BC company dry powder reagent, respectively. The absolute count and percentage of CD4 cells were analyzed by BD FACSCalibur flow cytometry and Multiset software program. SPSS software and Bland-Altman diagram were used to analyze the consistency of the two reagents. Results the average count of CD4 cells by BC and BD was (506.3 鹵605.3) / 渭 l (102 / 458) and (501.0 鹵610.3) / 渭 l / 渭 l respectively. Pearson correlation coefficient was 0.984, and the average deviation was 5.3 / 渭 l (95CIv-102.3113.0). Pearson correlation coefficient was very good. The average deviation was 0.66% (95% CI: -3.6%). The sensitivity and specificity of BC dry powder reagent with CD4 cell count equal to 350 / 渭 l were 95.8% and 98.4%, respectively. Conclusion compared with BD reagent, the absolute number and percentage of CD4 cells detected by FACSCalibur flow cytometry have good correlation and consistency. The preservation and transportation of dry powder reagent do not need cold chain, so it is suitable for popularization and application of basic unit.
【作者單位】： 云南省疾病預(yù)防控制中心性病艾滋病防制所;
【基金】：中美艾滋病防治合作項(xiàng)目(GAP項(xiàng)目)
【分類號(hào)】：R512.91

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