本文選題：丹參提取物 + 骨髓間充質(zhì)干細(xì)胞�。� 參考：《中國(guó)骨質(zhì)疏松雜志》2017年08期

【摘要】：目的研究丹參提取物對(duì)骨髓間充質(zhì)干細(xì)胞增殖及成骨分化的作用。方法采用全骨髓細(xì)胞接種法和貼壁純化培養(yǎng)骨髓間充質(zhì)干細(xì)胞,將濃度為0.2、0.4、0.8、1.6 mg/m L和3.2 mg/m L的丹參提取物加入到骨髓間充質(zhì)干細(xì)胞中,觀(guān)察不同濃度的丹參提取物對(duì)骨髓間充質(zhì)干細(xì)胞的增殖作用;采用0.4、0.8 mg/m L和3.2 mg/m L的丹參提取物誘導(dǎo)骨髓間充質(zhì)干細(xì)胞向成骨細(xì)胞分化,連續(xù)誘導(dǎo)21 d,利用ALP試劑盒測(cè)定成骨細(xì)胞內(nèi)ALP活性和茜素紅染色觀(guān)察成骨細(xì)胞鈣化結(jié)節(jié)情況;采用Western bloting法測(cè)定成骨細(xì)胞相關(guān)蛋白BMP-2和Runx的含量。結(jié)果不同濃度的丹參提取物均能夠促進(jìn)骨髓間充質(zhì)干細(xì)胞的增殖,丹參提取物濃度0.8 mg/m L時(shí)對(duì)骨髓間充質(zhì)干細(xì)胞的增殖作用最強(qiáng);ALP測(cè)定結(jié)果發(fā)現(xiàn)0.4、0.8 mg/m L和3.2mg/m L的丹參提取物均能夠提高ALP活性,丹參提取物濃度0.8 mg/m L時(shí)ALP活性最高;茜素紅染色結(jié)果發(fā)現(xiàn)0.4、0.8 mg/m L和3.2 mg/m L丹參提取物均能夠促進(jìn)成骨細(xì)胞的鈣化,丹參提取物濃度為0.8 mg/m L時(shí)鈣化結(jié)節(jié)數(shù)量最多;Western bloting結(jié)果表明,0.8 mg/m L的丹參提取物能夠促進(jìn)成骨細(xì)胞分化相關(guān)蛋白BMP-2和Runx-2表達(dá)。結(jié)論丹參提取物能夠促進(jìn)大鼠骨髓間充質(zhì)干細(xì)胞的增殖和成骨細(xì)胞分化作用。
[Abstract]:Objective to study the effects of Salvia miltiorrhiza extract on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. Methods Bone marrow mesenchymal stem cells (BMSCs) were cultured by whole bone marrow cell inoculation and adherent method. The extracts of Salvia miltiorrhiza (0.2G) 0.40.81.6 mg/m / L and 3.2 mg/m / L were added to bone marrow mesenchymal stem cells (BMSCs). The proliferation of bone marrow mesenchymal stem cells (BMSCs) was observed with different concentrations of Salvia miltiorrhiza extract, and the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts was induced by 0.40.8-and 3.2 mg/m / L Danshen extract. ALP activity and alizarin red staining were used to detect calcified nodules in osteoblasts and the contents of BMP-2 and Runx in osteoblasts were determined by Western bloting assay. Results different concentrations of Salvia miltiorrhiza extract could promote the proliferation of bone marrow mesenchymal stem cells. The proliferation of bone marrow mesenchymal stem cells was the strongest when the extract concentration of Salvia miltiorrhiza was 0. 8 mg/m / L. The results showed that the extract of 0. 4 ~ 0. 8 mg/m / L and 3.2mg/m / L could increase the activity of ALP, and the concentration of 0. 8 mg/m / L of Salvia miltiorrhiza had the highest activity of ALP. The results of alizarin red staining showed that 0.40.8 mg/m / L and 3.2 mg/m / L Salvia miltiorrhiza extracts could promote the calcification of osteoblasts. When the extract concentration of Salvia miltiorrhiza was 0. 8 mg/m / L, the number of calcified nodules was the highest. The results showed that the extract of Salvia miltiorrhiza at 0. 8 mg/m / L could promote the expression of BMP-2 and Runx-2 proteins associated with osteoblast differentiation. Conclusion Salvia miltiorrhiza extract can promote the proliferation and osteoblast differentiation of rat bone marrow mesenchymal stem cells.
【作者單位】： 牡丹江醫(yī)學(xué)院紅旗醫(yī)院骨科;牡丹江醫(yī)學(xué)院內(nèi)分泌科;牡丹江醫(yī)學(xué)院;
【分類(lèi)號(hào)】：R580
，

本文編號(hào)：2066346