本文選題：高糖 + HK-�。� 參考：《安徽醫(yī)科大學(xué)學(xué)報》2016年04期

【摘要】：目的研究高糖誘導(dǎo)人腎近曲小管上皮細胞凋亡及其調(diào)控機制。方法以人腎近曲小管上皮細胞株HK-2為研究對象,隨機分為對照組、高糖組、甘露醇組。MTT、流式細胞術(shù)觀察細胞的增殖和凋亡狀況;ELISA法檢測細胞內(nèi)C.aspase的活性;Westem blot法分析B淋巴細胞瘤-2(B1-2)及Caspase家族蛋白的表達變化。結(jié)果與對照組比較,高糖組能抑制HK-2細胞的體外增殖,下調(diào)抑制凋亡作用的蛋白Bcl-2表達,上調(diào)促凋亡作用的蛋白Bax、Bak表達(P0.05);流式細胞術(shù)顯示HK-2細胞周期有明顯的變化,且隨葡萄糖濃度的升高中期凋亡和末期凋亡所占的比例明顯上升(P0.01),并且Caspase酶原降解及活性表達也呈上升趨勢(P0.01)。結(jié)論高糖能抑制HK-2細胞體外增殖,并誘導(dǎo)其凋亡,其機制可能是通過Bcl-2及Caspase家族調(diào)控HK-2細胞的凋亡。
[Abstract]:Objective to study the apoptosis of human proximal tubule epithelial cells induced by high glucose and its regulatory mechanism. Methods Human renal proximal tubule epithelial cell line HK-2 was randomly divided into control group and high glucose group. In mannitol group, the proliferation and apoptosis of cells were observed by flow cytometry. The activity of C.aspase was detected by Elisa and the expression of Caspase family proteins in B lymphocytoma (-2mb _ (1-2) and Caspase family were detected by Elisa. Results compared with the control group, the high glucose group could inhibit the proliferation of HK-2 cells in vitro, down-regulate the expression of Bcl-2, and up-regulate the expression of apoptosis-promoting protein Bax-Bak, and flow cytometry showed that the cell cycle of HK-2 cells changed significantly. With the increase of glucose concentration, the proportion of apoptosis in metaphase and late stage of apoptosis increased significantly, and the degradation of Caspase and the expression of Caspase activity also tended to increase. Conclusion High glucose can inhibit the proliferation and induce apoptosis of HK-2 cells in vitro, and its mechanism may be the regulation of apoptosis of HK-2 cells by Bcl-2 and Caspase family.
【作者單位】： 九江學(xué)院附屬醫(yī)院檢驗科;九江學(xué)院附屬醫(yī)院醫(yī)保辦;安徽省/省部共建教育部重要遺傳病基因資源利用重點實驗室;
【基金】：國家自然科學(xué)基金(編號:81272399)
【分類號】：R587.2;R446

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相關(guān)期刊論文 前2條

1 曹軍軍;楊茂偉;郭寶磊;梁單;;高糖通過線粒體途徑誘導(dǎo)成骨細胞凋亡[J];中國生物化學(xué)與分子生物學(xué)報;2012年12期

2 袁育s，

本文編號：2049890