本文選題：長鏈非編碼RNA + 關(guān)節(jié)炎　； 參考：《重慶醫(yī)學》2017年17期

【摘要】：目的探究長鏈非編碼RNA(IncRNA)在類風濕性關(guān)節(jié)炎(RA)患者外周血單個核細胞(PBMCs)和健康人PBMCs中的差異表達,并對其分析以進一步闡明RA的發(fā)病機制。方法選取2016年1-4月在該院風濕免疫科就診的RA患者22例作為觀察組,22例健康人作為對照組。采用Agilent Human IncRNA芯片測定5例RA患者和5例健康人的PBMCs中的IncRNA和mRNA的差異表達;利用GO以及Pathway分析檢測出差異表達的IncRNA的功能分布,構(gòu)建起IncRNA-mRNA的共表達網(wǎng)絡(luò),采用Trans-與Cis-預(yù)測其中與RA發(fā)病相關(guān)的IncRNA。結(jié)果 IncRNA在RA患者的PBMCs和健康人的PBMCs中共存在1 623條差異表達,mRNA差異表達則有851條。GO、Pathway分析表明,差異表達的mRNA的主要功能是參與蛋白激酶、核苷酸、金屬離子的結(jié)合和轉(zhuǎn)錄調(diào)節(jié),還參與B細胞受體信號通路、TNF信號通路的調(diào)節(jié)。經(jīng)過Cis-和Trans-分析預(yù)測和Real-time PCR驗證后找到3個IncRNA可能與RA發(fā)病有關(guān),分別為NONHSAT 120696、uc.80+、NONHSAT 031501。結(jié)論 IncRNA在RA患者PBMCs和健康人PBMCs中存在差異表達,NONHSAT 120696、uc.80+、NONHSAT 031501 3個IncRNA可能與RA的發(fā)病有關(guān)。
[Abstract]:Objective to investigate the differential expression of long chain noncoding RNAs (IncRNAs) in peripheral blood mononuclear cells (PBMCs) of patients with rheumatoid arthritis (RA) and healthy PBMCs, and to further elucidate the pathogenesis of RA. Methods 22 RA patients from January to April 2016 were selected as control group. The differential expression of IncRNA and mRNA in PBMCs of 5 RA patients and 5 healthy controls were detected by Agilent Human IncRNA microarray, the functional distribution of differentially expressed IncRNAs was detected by go and Pathway analysis, and the coexpression network of IncRNA-mRNA was constructed. Trans- and Cis- were used to predict the incidence of RA. Results there were 1 623 differentially expressed mRNAs of IncRNA in PBMCs of RA patients and healthy people, while 851 differentially expressed mRNAs showed that the main function of differentially expressed mRNA was involved in protein kinase and nucleotides. The binding and transcriptional regulation of metal ions also participate in the regulation of B cell receptor signaling pathway and TNF signaling pathway. After Cis- and Trans-analysis prediction and Real-time PCR verification, it was found that three IncRNAs might be associated with RA, namely NONHSAT 120696uc.80 and NONHSAT031501. Conclusion there is a differential expression of IncRNA in PBMCs of RA patients and healthy people. NONHSAT 120696uc.80 NoNHSAT 031501 3 IncRNAs may be related to the pathogenesis of RA.
【作者單位】： 南華大學附屬第一醫(yī)院風濕免疫科;南華大學附屬第一醫(yī)院骨科;
【分類號】：R593.22

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