本文選題：葡萄糖 + 傳感器��； 參考：《中國藥科大學學報》2017年04期

【摘要】：構(gòu)建一種二氧化錳納米片與葡萄糖氧化酶雜化自指示比色探針,在弱酸性條件下,葡萄糖氧化酶專屬性催化氧化葡萄糖,定量產(chǎn)生葡萄糖酸和過氧化氫,生成的過氧化氫有效分解二氧化錳納米片,探針體系特征吸收波長(374 nm)處吸收度降低。吸收度變化量與葡萄糖濃度呈線性相關(guān),線性范圍1~20μmol/L,相關(guān)系數(shù)R~2=0.990 1,檢測限低至0.1μmol/L。探針對葡萄糖具有高選擇性和靈敏度,血清樣品中常見的氨基酸、陰離子、陽離子及蛋白質(zhì)等小分子物質(zhì)造成干擾均可忽略,實際樣品測定具有前處理簡單、操作方便、快速等特點。此外,鑒于二氧化錳的氧化還原特性以及酶催化體系的多樣性,酶-二氧化錳納米片雜化探針平臺具有普適性,可設(shè)計構(gòu)建多種分析物的比色傳感器。
[Abstract]:A hybrid self-directed colorimetric probe of manganese dioxide nanoparticles and glucose oxidase was constructed. Glucose oxidase was used to catalyze the oxidation of glucose to quantitatively produce gluconic acid and hydrogen peroxide under the condition of weak acidity. The resulting hydrogen peroxide effectively decomposes manganese dioxide nanoparticles and decreases the absorption at the characteristic absorption wavelength of the probe system at 374 nm. There was a linear correlation between the absorbency and glucose concentration. The linear range was 1 ~ 20 渭 mol / L, the correlation coefficient was RV 20.990 1, and the detection limit was as low as 0.1 渭 mol 路L ~ (-1). The probe has high selectivity and sensitivity to glucose. The interference caused by small molecules such as amino acids, anions, cations and proteins in serum samples can be ignored. Speed, etc. In addition, in view of the redox characteristics of manganese dioxide and the diversity of enzyme catalytic system, the hybrid probe platform of enzyme manganese dioxide nanochip is universal and can be used to design and construct colorimetric sensors for many kinds of analytes.
【作者單位】： 中國藥科大學藥物分析學教研室;中國藥科大學藥物質(zhì)量與安全預(yù)警教育部重點實驗室;南開大學藥物化學生物學國家重點實驗室;
【基金】：國家自然科學基金資助項目(No.21305161) 江蘇省自然科學基金資助項目(No.BK20130643) 藥物質(zhì)量與安全預(yù)警教育部重點實驗室開放課題資助項目(DQCP2015MS06) 藥物化學生物學國家重點實驗室(南開大學)開放基金資助項目(No.20130404)~~
【分類號】：R587.1;TP212.3
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本文編號：2036528