本文選題：營養(yǎng)物質 + 產卵量��； 參考：《合肥工業(yè)大學》2017年碩士論文

【摘要】：隨著社會發(fā)展,飲食結構也在發(fā)生改變,伴隨著高營養(yǎng)物質的過量攝入,肥胖的比例在不斷上升。與此同時,不孕不育的比例也在逐漸上升。由于營養(yǎng)對生殖發(fā)育具有重要影響,我們推測飲食誘導的脂肪沉積與生育率有重要影響。本研究利用模式生物線蟲研究了上述問題,通過飲食添加營養(yǎng)物質,然后觀察線蟲的脂肪沉積和產卵量(brood size)變化,并研究了相關機制。首先調查了葡萄糖、膽固醇、棕櫚酸、油酸四種營養(yǎng)物質對線蟲產卵總量及脂肪沉積的影響。葡萄糖和棕櫚酸添加后線蟲的產卵總量下降,但是脂肪沉積增加。通過改變線蟲的腸道菌群,我們研究了飲食添加葡萄糖、棕櫚酸誘導線蟲產卵總量和脂肪沉積表型的影響。用不同種類的大腸桿菌喂食線蟲,同時添加葡萄糖和棕櫚酸,調查產卵量和脂肪沉積表型。結果表明改變線蟲的腸道菌群,添加葡萄糖或棕櫚酸后線蟲依然會出現(xiàn)產卵總量下降,脂肪沉積增加的表型。接下來我們探討了飲食誘導線蟲產卵總量下降的過程中,脂肪沉積改變的必要性。我們發(fā)現(xiàn),葡萄糖、棕櫚酸直接引起的線蟲產卵總量的下降,不依賴脂肪沉積的改變;而膽固醇則可能是通過脂肪沉積增加而引起產卵量的下降。最后,我們探討了調控上述現(xiàn)象的分子機制。通過定量PCR相關基因及突變體的研究,我們發(fā)現(xiàn)營養(yǎng)物質葡萄糖、棕櫚酸誘導線蟲產卵總量下降與蛋白質糖基化轉移酶ogt-1和溶酶體組織蛋白酶cpl-1有關。綜上所述,通過本文研究我們得出營養(yǎng)物質的添加會影響線蟲產卵總量的改變,其中葡萄糖和棕櫚酸添加情況下,誘導線蟲產卵總量下降不依賴脂肪沉積的增加。而是通過蛋白質糖基化及溶酶體組織蛋白酶的作用。有利于研究由于飲食誘導肥胖導致繁殖力改變的相關研究、預防和治療。
[Abstract]:With the development of the society, the diet structure is also changing, with the excessive intake of high nutrients, the proportion of obesity is increasing. At the same time, the proportion of infertility is increasing. Because of the important effect of nutrition on reproductive development, we speculate that the diet induced fat deposition and fertility have an important impact. The above problems were studied with model biological nematodes, and nutrients were added to the diet. Then the fatty deposits and brood size changes of nematodes were observed and the related mechanisms were studied. First, the effects of four nutrients, glucose, cholesterol, palmitic acid and oleic acid, on the amount of egg production and fat deposition of line worms. After the addition of the nematode, the total amount of spawning decreased, but the fat deposition increased. By changing the intestinal flora of the nematode, we studied the effects of dietary glucose, palmitic acid induced oviposition and the fat deposition phenotype. Feeding nematodes with different kinds of Escherichia coli, adding glucose and palmitic acid to investigate the amount of eggs and fat. The results showed that the nematode colony of the nematode was changed. After the addition of glucose or palmitic acid, the total amount of oviposition decreased and the fat deposition was increased. Then we explored the necessity of the change in the fat deposition in the process of reducing the total amount of oviposition of the nematode. We found that glucose and palmitic acid are directly caused. The decrease of the total amount of egg laying in the nematode is not dependent on the change in the fat deposition, and the cholesterol may be caused by the increase of the fat deposition. Finally, we explored the molecular mechanism to regulate the above phenomenon. Through the study of the quantitative PCR related genes and mutants, we found the nutrient substance glucose and the palmitic acid induced nematodes. The decrease of total egg production is related to the protein glycosyltransferase ogt-1 and lysosome cathepsin Cpl-1. In this paper, we conclude that the addition of nutrients will affect the change of the total amount of egg laying in the nematode. In the case of glucose and palmitic acid, the decrease of the total amount of oviposition of nematodes is not dependent on the increase of fat deposition. It is through protein glycosylation and lysosomal cathepsin. It is beneficial to study the related research, prevention and treatment of fecundity caused by diet induced obesity.
【學位授予單位】：合肥工業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R589.2

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