本文選題：梓醇 + 成骨細(xì)胞�。� 參考：《中國(guó)藥房》2016年10期

【摘要】：目的:研究中藥單體梓醇在成骨細(xì)胞(OB)-破骨細(xì)胞(OC)共育體系中對(duì)OC活性、凋亡的影響及其作用機(jī)制。方法:分別選用1~3 d齡和5~7 d齡SD乳鼠分離培養(yǎng)OB和OC,建立OB-OC共育體系。通過(guò)倒置顯微鏡觀察0(空白對(duì)照)、0.05、0.5、5、50、100 mg/L梓醇培養(yǎng)48、72、96 h后OC的骨吸收陷窩數(shù)目,以反映OC活性;以0(空白對(duì)照)、0.05 mg/L梓醇培養(yǎng)細(xì)胞48、72、96h,檢測(cè)OC中抗酒石酸酸性磷酸酶(TRACP)活性,計(jì)算OC的凋亡率;以0(空白對(duì)照)、0.05 mg/L梓醇培養(yǎng)細(xì)胞并檢測(cè)OB中骨保護(hù)素(OPG)m RNA的表達(dá)。結(jié)果:在OB-OC共育體系中,0.05~50 mg/L梓醇作用下所形成骨吸收陷窩數(shù)目顯著低于空白對(duì)照(P0.01),表明梓醇可抑制OC活性,其中質(zhì)量濃度為0.05 mg/L時(shí)作用最明顯。與空白對(duì)照比較,0.05 mg/L梓醇作用后OC中TRACP的活性降低、OC的凋亡率升高(P0.05);OB的OPG m RNA表達(dá)上調(diào)(P0.01)。結(jié)論:在OB-OC共育體系中,梓醇可抑制OC活性、誘導(dǎo)OC凋亡,其機(jī)制可能與上調(diào)OB的OPG m RNA表達(dá)有關(guān)。
[Abstract]:Aim: to study the effect and mechanism of catalpol on OC activity and apoptosis in osteoblast OBM-osteoclast co culture system. Methods: OB and OC were isolated and cultured from SD rats aged 3 days and 57 days, respectively, and the co-culture system of OB-OC was established. The number of bone resorption lacunae of OC was observed by inverted microscope, and the activity of OC was measured by 0. 05 mg / L catalpol 50 mg / L catalpol culture for 48 ~ 722 ~ 96 h, and the activity of tartrate-resistant acid phosphatase TRACPP was detected by 0 (0. 05 mg / L catalpol culture for 48? 722? 96 h). The apoptosis rate of OC was calculated and the expression of osteoprotegerin (OPG) mRNA in OB was detected by 0 (control group) 0.05 mg / L catalpol. Results: in OB-OC co-culture system, the number of bone resorption lacunae formed under the action of 0.05 mg / L catalpol was significantly lower than that of the control group (P0.01), indicating that catalpol could inhibit OC activity, especially when the mass concentration was 0.05 mg / L. Compared with the control group, the activity of TRACP in OC decreased after treatment with 0.05 mg / L catalpol. The apoptosis rate of OC increased. The expression of OPG mRNA of P0.05 and OB upregulated P0.01. Conclusion: catalpol can inhibit OC activity and induce OC apoptosis in OB-OC co-culture system. The mechanism may be related to the up-regulation of OB OPG mRNA expression.
【作者單位】： 廣東食品藥品職業(yè)學(xué)院;暨南大學(xué)藥學(xué)院;
【基金】：廣東省醫(yī)學(xué)科學(xué)技術(shù)研究基金項(xiàng)目(No.B2015063) 廣東食品藥品職業(yè)學(xué)院自然科學(xué)基金項(xiàng)目(No.2014YZ003)
【分類號(hào)】：R580

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