本文選題：氟 + 成骨細(xì)胞�。� 參考：《中國地方病防治雜志》2017年03期

【摘要】：目的探討氟調(diào)控成骨細(xì)胞內(nèi)質(zhì)網(wǎng)應(yīng)激信號(hào)通路對(duì)細(xì)胞增殖凋亡分化的影響。方法培養(yǎng)成骨細(xì)胞MC3T3-E1,0、1、2、4、8、16、32、64 mg F-/L組9個(gè)不同的氟濃度處理成骨細(xì)胞MC3T3-E1 2、7、14 d后,每個(gè)時(shí)間段設(shè)置對(duì)照組,CCK8檢測細(xì)胞的增殖;2、8、20 mg F-/L處理細(xì)胞,流式細(xì)胞儀檢測不同濃度氟對(duì)成骨細(xì)胞MC3T3-E1凋亡的影響;qRT-PCR檢測細(xì)胞中與分化相關(guān)的基因的表達(dá);Western blot檢測內(nèi)質(zhì)網(wǎng)應(yīng)激信號(hào)通路相關(guān)蛋白的表達(dá)。結(jié)果低濃度氟(2、4mg F-/L)和中濃度氟(8 mg F-/L)顯著增強(qiáng)細(xì)胞的增殖(P0.05),高濃度氟(16 mg F-/L)顯著降低細(xì)胞的增殖(P0.05);2、8、20 mg F-/L處理成骨細(xì)胞MC3T3-E1,細(xì)胞凋亡率顯著高于0 mg F-/L(P0.01),且隨著濃度的增加凋亡率變大;2 mg F-/L促進(jìn)細(xì)胞內(nèi)ALP、OCN、Runx2和Osterix的mRNA表達(dá),20 mg F-/L則抑制上述因子的mRNA表達(dá)(P0.01);2、8、20 mg F-/L處理MC3T3-E1細(xì)胞中Xbp1、AFT4、Bip和PERK的蛋白表達(dá)量都高于0 mg F-/L,其中2 mg F-/L處理細(xì)胞中Xbp1和AFT4蛋白表達(dá)最多(P0.01),8 mg F-/L處理細(xì)胞中PERK的蛋白表達(dá)量最多(P0.01),20 mg F-/L處理細(xì)胞中Bip蛋白表達(dá)量最多(P0.01)。結(jié)論低濃度氟促進(jìn)成骨細(xì)胞MC3T3-E1增殖和分化,高濃度則抑制,細(xì)胞凋亡率隨氟濃度增加而增加,不同氟濃度處理促進(jìn)成骨細(xì)胞中內(nèi)質(zhì)網(wǎng)應(yīng)激信號(hào)通路相關(guān)蛋白的表達(dá)。
[Abstract]:Objective to investigate the effect of fluoride on proliferation, apoptosis and differentiation of osteoblast endoplasmic reticulum (ER) stress signaling pathway. Methods Osteoblast MC3T3-E1OOOOOOOVEROVICAL cells were treated with 9 different concentrations of fluorine for 714 days. The proliferation of MC3T3-E1 cells was assayed by CCK8 at each time period, and the proliferation of MC3T3-E1 cells treated with F-R / L was detected by CCK8 at each time period, and the proliferation of MC3T3-E1 / L cells was measured at each time interval after treatment with 9 different concentrations of fluorine, and the proliferation of MC3T3-E1 / L cells was measured. Effects of different concentrations of fluoride on apoptosis of osteoblasts MC3T3-E1 the expression of differentiation-related genes in osteoblasts was detected by qRT-PCR and the expression of ERS-related proteins was detected by Western blot. Results the cell proliferation was significantly enhanced by 4 mg F / L and 8 mg F / L at low and middle concentrations, and the proliferation of MC3T3-E1 was significantly decreased by high concentration of F / L (16 mg F / L). The apoptosis rate of MC3T3-E1 cells was significantly higher than that of MC3T3-E1 treated with 820 mg F-P / L of high concentration of fluorine. The apoptosis rate of MC3T3-E1 cells was significantly higher than that of MC3T3-E1 cells with concentration of 0 mg F-P / L, and the apoptosis rate of MC3T3-E1 cells was significantly higher than that of MC3T3-E1 cells. Increased apoptosis rate increased by 2 mg F / L and enhanced mRNA expression of Osterix and Osterix mRNA in MC3T3-E1 cells treated with 2 mg F / L and 20 mg F-P / L, the protein expression of Xbp1AFT4Bip and Perk in MC3T3-E1 cells was significantly higher than that in MC3T3-E1 cells treated with 2 mg F-r-L, and the protein expression of Xbp1AFT4Bip and PERK in MC3T3-E1 cells was significantly higher than that in MC3T3-E1 cells treated with 2 mg F-rL. Xbp1 and AFT4 proteins were most expressed in the cells treated with P0.01mg / L and P0.01mg / L, and the highest expression of Bip protein was found in the cells treated with P0.01mg / L and 20 mg / L P0.01mg / L respectively, and the highest expression of Bip protein was found in the cells treated with P0.01mg / L and P0.01mg / L respectively. Conclusion low concentration of fluoride can promote the proliferation and differentiation of osteoblasts MC3T3-E1, and high concentration of fluoride can inhibit the proliferation and differentiation of MC3T3-E1. The apoptosis rate of MC3T3-E1 cells increases with the increase of fluoride concentration. Different fluoride concentrations can promote the expression of ER stress signaling pathway related protein in osteoblasts.
【作者單位】： 齊齊哈爾醫(yī)學(xué)院附屬第三醫(yī)院骨科;
【基金】：齊齊哈爾市科學(xué)技術(shù)計(jì)劃項(xiàng)目(SFZD-2014068)
【分類號(hào)】：R599.1

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本文編號(hào)：2002382