本文選題：運(yùn)動 + 糖尿病　； 參考：《廣西醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的通過建立2型糖尿病SD大鼠模型,探討不同運(yùn)動量對糖尿病肝臟組織形態(tài)和肝臟中炎癥通路關(guān)鍵因子TLR4和NF-KB表達(dá)的影響,為運(yùn)動改善糖尿病的分子生物學(xué)機(jī)制提供新的實(shí)驗依據(jù)。方法(1)將50只SD大鼠隨機(jī)分為對照組(NC)10只、糖尿病模型組(DM)10只和糖尿病小運(yùn)動量組(LIE)10只、糖尿病中運(yùn)動量組(MIE)10只、糖尿病大運(yùn)動量組(HIE)10只。NC組給予普通膳食,余四組給予高糖高脂飲食(常規(guī)飼料加5%的蛋黃、10%的蔗糖、10%的煉豬油),飼養(yǎng)4周后,NC組予注射檸檬酸緩沖液,其余四組給予小劑量STZ腹腔注射建立2型糖尿病大鼠模型。(2)隨機(jī)選取兩只DM組大鼠,以SD大鼠所能堅持的最長游泳時間(即力竭時間)為標(biāo)志,來安排運(yùn)動量的大小,最長游泳時間的劃定標(biāo)準(zhǔn)為最終大鼠能夠保持在水面上的時間小于8秒。HIE組的運(yùn)動量以力竭時間為標(biāo)準(zhǔn),MIE組、LIE組的運(yùn)動量分別以力竭時間的70%和40%為標(biāo)準(zhǔn)。本實(shí)驗中具體時間為：30min、21min、12min。各運(yùn)動組按預(yù)先規(guī)定好的運(yùn)動量進(jìn)行訓(xùn)練,1次/天,6天/周,共6周。實(shí)驗結(jié)束后,處死大鼠,采集血液和肝臟組織標(biāo)本。檢測大鼠體重、肝重、空腹血糖。用RT-PCR半定量法測定TLR4和NF-KB mRNA的表達(dá)。用免疫組化法測定肝臟TLR4和NF-kB蛋白的表達(dá)。肝臟組織用福爾馬林固定、石臘包埋、切片后進(jìn)行HE染色,顯微鏡下分析肝臟組織病理形態(tài)變化。結(jié)果(1)實(shí)驗結(jié)束后,各組大鼠肝臟組織HE染色結(jié)果顯示：NC組大鼠肝臟組織未見異常；DM組、LIE組、MIE組和HIE組四組糖尿病大鼠肝臟組織均出現(xiàn)2型糖尿病肝損害的病理改變；LIE組、MIE組和HIE組三組運(yùn)動干預(yù)組大鼠的肝臟組織形態(tài)較DM組大鼠有所恢復(fù),其中以MIE組大鼠恢復(fù)程度最為顯著。(2)實(shí)驗結(jié)束后,與NC組相比,四組糖尿病大鼠的體重和肝重出現(xiàn)顯著地降低(P0.05),空腹血糖出現(xiàn)顯著地升高(P0.05),肝臟組織中TLR4和NF-kB的mRNA出現(xiàn)顯著地升高(P0.05),肝臟組織中TLR4和NF-kB的蛋白表達(dá)出現(xiàn)顯著地升高(P0.05)；與DM組相比,各運(yùn)動組的血糖出現(xiàn)顯著降低(P0.05)、肝臟組織中TLR4和NF-kB的mRNA均有不同程度的降低(P0.05),肝臟組織中TLR4和NF-kB的蛋白均有不同程度的降低(P0.05)；與LIE組和HIE組相比,MIE組大鼠的空腹血糖出現(xiàn)顯著降低(P0.05)、肝臟組織中TLR4和NF-kB的mRNA出現(xiàn)顯著降低(P0.05),肝臟組織中TLR4和NF-kB的蛋白出現(xiàn)顯著降低(P0.05)。結(jié)論6周的游泳訓(xùn)練能夠降低2型糖尿病大鼠的血糖,降低肝臟TLR4和NF-kB的mRNA和蛋白的表達(dá),改善肝臟的炎癥狀態(tài),恢復(fù)肝臟組織學(xué)形態(tài),緩解糖尿病的發(fā)展。在三種不同大小的運(yùn)動量中,中等運(yùn)動量的干預(yù)效果最為顯著。
[Abstract]:Objective to investigate the effects of different exercise doses on the expression of TLR4 and NF-KB in diabetic liver and the key factors of inflammatory pathway in the liver of type 2 diabetic SD rats. To provide a new experimental basis for exercise to improve the molecular biological mechanism of diabetes. Methods 1) 50 SD rats were randomly divided into control group (n = 10), diabetic model group (n = 10), diabetic group (n = 10), small exercise group (n = 10), exercise volume group (n = 10), HIEE group (n = 10) and control group (n = 10). The remaining four groups were given a high-sugar and high-fat diet (conventional diet plus 10% sucrose 10% lard, 5% egg yolk). After 4 weeks of feeding, the NC group was given citric acid buffer. The other four groups were given small dose of STZ intraperitoneally to establish type 2 diabetic rat model.) two diabetic rats were randomly selected. The maximum swimming time (i.e. exhaustion time) of SD rats was used as the symbol to arrange the amount of exercise. The standard of the longest swimming time was that the rats could stay on the water for less than 8 seconds. The exercise quantity of the HIE group was 70% of the exhaustion time and 40% of the exhaustion time in the mie group. In this experiment, the specific time is: 30 min, 21 min, 12 min. The exercise groups were trained once a day for 6 days a week according to the prescribed amount of exercise in advance for a total of 6 weeks. At the end of the experiment, the rats were killed and blood and liver tissue specimens were collected. Body weight, liver weight and fasting blood glucose were measured. The expression of TLR4 and NF-KB mRNA was determined by RT-PCR semi-quantitative method. The expression of TLR4 and NF-kB in liver was detected by immunohistochemical method. The liver tissue was fixed with formalin, embedded in paraffin, stained with HE, and the histopathological changes of liver were analyzed under microscope. Results 1) after the experiment, The results of HE staining in liver tissue of rats in each group showed that there was no abnormality in liver tissue of rats in the control group. The liver tissues of the Li group and the HIE group showed hepatic damage of type 2 diabetes mellitus. The pathological changes of liver tissue of rats with type 2 diabetes were found in lie group, mie group and HIE group. The liver histomorphology of the three groups of exercise intervention group was better than that of the DM group. At the end of the experiment, the recovery degree of MIE group was the most significant, and compared with NC group, the recovery degree of MIE group was higher than that of NC group. In the four groups of diabetic rats, the body weight and liver weight decreased significantly (P 0.05), the fasting blood glucose increased significantly (P 0.05), the mRNA of TLR4 and NF-kB in liver tissue increased significantly (P 0.05), and the protein expression of TLR4 and NF-kB in liver tissue increased significantly. Compared with DM group, P0. 05, P 0. 05, P 0. 05, P 0. 05. The blood glucose of each exercise group decreased significantly (P0.05), the mRNA of TLR4 and NF-kB in liver tissue decreased in varying degrees, and the protein of TLR4 and NF-kB in liver tissue decreased in varying degrees; compared with LIE group and HIE group, the empty stomach of rats in mie group was lower than that in LIE group and HIE group. There was a significant decrease in blood glucose (P 0.05), TLR4 and NF-kB (mRNA) in liver tissue (P 0.05), and the protein of TLR4 and NF-kB (P 0.05) in liver tissue. Conclusion Swimming training for 6 weeks can reduce the blood glucose, decrease the expression of mRNA and protein in TLR4 and NF-kB, improve the inflammatory state of liver, restore the histological morphology of liver and alleviate the development of diabetes mellitus in rats with type 2 diabetes mellitus. Among the three kinds of exercise, the intervention effect of moderate exercise was the most significant.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R587.1

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