本文選題：硫化氫 + 硫烷硫��； 參考：《新鄉(xiāng)醫(yī)學院》2017年碩士論文

【摘要】：背景系統(tǒng)性紅斑狼瘡(SLE)是一種累及多系統(tǒng)損害的慢性自身免疫性疾病,狼瘡性腎炎(LN)是在SLE基礎上出現(xiàn)的腎臟損害,兩者發(fā)病機制主要與免疫系統(tǒng)異常相關。硫化氫(H_2S)為哺乳動物體內具有生物活性的氣體信號分子,在多種組織器官中均有分布。已有研究報道顯示H_2S在機體免疫調節(jié)中起一定作用,但關于H_2S與SLE的相關研究國內外卻鮮有報道。目的本研究擬通過檢測SLE患者和正常對照者血清中H_2S濃度,比較H_2S水平在SLE患者不同分組中的差異,探討血清中H_2S與SLE疾病發(fā)生發(fā)展的相關性。方法選擇2016年1月至2017年1月在河南省人民醫(yī)院腎病風濕免疫科就診的SLE患者119例,本院健康體檢者27例,收集所有研究對象的血清標本和一般資料。將SLE患者依據(jù)是否伴腎損害,分為無腎損害SLE組67例、LN組52例;依據(jù)SLEDAI積分分為A組(基本無活動組)、B組(輕度活動組)、C組(中重度活動組)。分別用去蛋白法和SSP4熒光探針法檢測研究對象血清中H_2S濃度。比較不同組間血清H_2S水平差異,多自變量logistic回歸分析SLE疾病活動的危險因素。數(shù)據(jù)分析采用SPSS18.0軟件包,以P0.05為差異有顯著性。結果1.各組間年齡及性別構成比均無顯著差異(P0.05)。無腎損害的SLE組患者補體C3、補體C4、血白細胞、紅細胞、血紅蛋白、血小板和血沉較LN組患者均無顯著差異(P0.05);C-反應蛋白水平低于LN組患者,有統(tǒng)計學差異(P0.05)。2.兩種方法檢測SLE組患者血清中H_2S水平均明顯低于對照組(P0.001)。LN組及無腎損害的SLE組患者血清中H_2S水平均低于對照組(P0.001)。LN組患者血清H_2S水平顯著低于無腎損害的SLE組(P0.01)3.兩種方法檢測C組患者血清H_2S水平均顯著低于A組和B組(P0.001)。去蛋白法檢測B組H_2S水平與A組和對照組比較無顯著差異;SSP4熒光探針法三組血清中H_2S水平均低于對照組(P0.05),B組H_2S水平低于A組(P0.001)。4.以SLE疾病是否活動為因變量,進行二元多自變量logistic回歸分析顯示,血清H_2S水平與SLE疾病活動呈負相關,是SLE疾病活動的獨立危險因素[β=-1.257,P0.01,95%CI(0.123,0.659)]結論1.血清H_2S水平降低與SLE疾病發(fā)生發(fā)展相關,是SLE疾病活動的獨立危險因素。2.SSP4熒光探針法對H_2S的檢測更靈敏,選擇性更高,在SLE病情分層方面更有意義。3.血清中H_2S在LN患者中顯著低于無腎損害的SLE患者,提示H_2S減少可能增加SLE患者合并腎損害的風險。
[Abstract]:Background systemic lupus erythematosus (SLE) is a chronic autoimmune disease involving multiple system damage. Lupus nephritis (LN) is a renal lesion based on SLE. The pathogenesis of these two diseases is mainly related to the abnormal immune system. H2S) is a biologically active gas signal molecule in mammals and is distributed in many tissues and organs. It has been reported that H2S plays an important role in immunomodulation, but there are few reports about H2S and SLE at home and abroad. Objective to investigate the relationship between serum H2S and the occurrence and development of SLE in patients with SLE and normal controls by comparing H2S levels in different groups of patients with SLE. Methods from January 2016 to January 2017, 119 patients with SLE were selected from the Department of Rheumatological Immunology for Nephropathy in Henan Provincial people's Hospital, and 27 patients were examined in our hospital. The serum samples and general data of all the subjects were collected. The patients with SLE were divided into 67 cases of LN group without renal damage and 52 cases of LN group according to whether they had renal damage or not. According to the SLEDAI score, they were divided into group A (basic inactive group) and group B (mild active group, group C (moderate and severe active group). The concentration of Hass in serum was detected by deproteinization and SSP4 fluorescence probe method respectively. The serum H2S levels were compared among different groups, and the risk factors of SLE disease activity were analyzed by multivariate logistic regression analysis. The data were analyzed by SPSS18.0 software package, with P0.05 as the significant difference. Result 1. There was no significant difference in age and sex ratio among groups (P 0.05). The levels of complement C3, complement C4, white blood cell, red blood cell, hemoglobin, platelet and erythrocyte sedimentation rate in SLE group without renal damage were significantly lower than those in LN group. The serum levels of H2S in the SLE group were significantly lower than those in the control group (P0.001N. LN) and the SLE group without renal damage. The serum H2S levels of the patients in the SLE group were significantly lower than those in the control group (P 0.001). The levels of H2S in the patients in the control group were significantly lower than those in the SLE group without renal damage (P 0.01). The serum H2S levels in group C were significantly lower than those in group A and group B (P 0.001). There was no significant difference in the level of H2S in group B compared with that in group A and control group. The levels of H2S in serum of three groups with SSP4 fluorescence probe method were lower than those of group B (P 0.05) and that of group B (P 0.001) .4.The level of H2S in group B was lower than that in group A (P 0.001) and that in group B was lower than that in group B (P 0.05). Taking the activity of SLE disease as dependent variable, logistic regression analysis showed that the serum H2S level was negatively correlated with the disease activity of SLE, and was an independent risk factor of SLE disease activity [尾 -1.257P0.01P0.01CI0.123C0.559] conclusion 1. The decrease of serum H2S level is related to the occurrence and development of SLE disease. It is an independent risk factor of SLE disease activity. 2. SSP4 fluorescence probe method is more sensitive and selective in the detection of H2S, and has more significance in the stratification of SLE disease. Serum H2S levels in LN patients were significantly lower than those in SLE patients without renal damage, suggesting that the decrease of H2S might increase the risk of renal damage in SLE patients.
【學位授予單位】：新鄉(xiāng)醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R593.241

【參考文獻】

相關碩士學位論文 前1條

1 付蘭英;H_2S通過PI_3K/AKT-NF-κB通路調節(jié)離體大鼠胰腺腺泡細胞TNF-α、IL-1β、IL-10的表達[D];第三軍醫(yī)大學;2013年

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