本文選題：Graves病 + 腸道菌群　； 參考：《蘭州大學(xué)》2017年碩士論文

【摘要】：目的:通過研究Graves病(graves diseases,GD)患者外周血中輔助性T細(xì)胞(helper T cell)Th17細(xì)胞頻率以及其腸道菌群中普雷沃氏菌(Prevotella Shan and Collins)與分節(jié)絲狀菌(Segmented Filamentous Bacteria,SFB)與正常人群組含量的差別,探討兩種細(xì)菌與該自身免疫性甲狀腺疾病發(fā)生與進(jìn)展的關(guān)系。方法:收集30例初發(fā)Graves患者的新鮮糞便及外周血作為GD組,30例健康對照者新鮮糞便樣品及外周血作為NC組作為研究樣本,即分為患者組與健康對照組。用試劑盒提取糞便中腸道菌群的總DNA,分別針對兩種細(xì)菌即普雷沃氏菌和分節(jié)絲狀菌,設(shè)計特異性引物,通過熒光定量PCR(Real-time PCR)技術(shù)對目的基因進(jìn)行擴(kuò)增,對比兩組兩種細(xì)菌的含量差異。采用流式細(xì)胞技術(shù)分析外周血中的Th17細(xì)胞進(jìn)行分析,檢測其百分率,對比兩組外周血中Th17細(xì)胞比例的差異。結(jié)果:初發(fā)Graves患者組(GD組)的Th17細(xì)胞比例明顯高于健康對照人群(NC組)(12.46+3.14 vs.7.05+2.12,P0.05),GD組患者外周血Th17細(xì)胞百分率與血清中的FT3,FT4呈顯著正相關(guān)性(r=0.682,r=0.567,P均0.05),與其他甲狀腺功能指標(biāo)無統(tǒng)計學(xué)差異(P0.05)。GD組患者腸道菌群中SFB含量約為NC組的2.384×103倍(RQ值為2.384×103),GD組腸道菌群中普雷沃氏菌含量約為NC組的1.825×103倍(RQ值為1.825×103)。結(jié)論:1.Graves病患者外周血中Th17細(xì)胞比例顯著高于正常對照組,且與外周血FT3、FT4呈顯著正相關(guān)關(guān)系;2.Graves患者腸道菌群中分節(jié)絲狀菌和普雷沃氏菌的含量顯著高于正常對照組;
[Abstract]:Objective: To explore the difference between the Th17 cell frequency of auxiliary T cells (helper T cell) in peripheral blood of patients with Graves disease (Graves diseases, GD) and the difference between the contents of Poulet Was bacteria (Prevotella Shan and) and segmental filamentous bacteria in the intestinal microflora and the normal group, and explore the two bacteria and the self. Methods: the relationship between the occurrence and progress of immune thyroid disease. Methods: the fresh feces and peripheral blood of 30 primary Graves patients were collected as group GD. The samples of fresh feces and peripheral blood were used as the research samples in the group of NC, which were divided into the patient group and the healthy control group. The total DNA of the intestinal microflora in the feces was extracted with the test kit. The specific primers were designed for two kinds of bacteria, namely, perveviella and segmental filamentous bacteria. The target genes were amplified by the fluorescence quantitative PCR (Real-time PCR) technique. The difference of the content of the two bacteria in the two groups was compared. The percentage of the Th17 cells in the peripheral blood was analyzed by flow cytometry, and the percentage of the Th17 cells was detected and compared with the Th17 in the peripheral blood. Results: the proportion of cells in the primary Graves group (group GD) was significantly higher than that in the healthy control group (group NC) (group NC) (12.46+3.14 vs.7.05+2.12, P0.05). The percentage of Th17 cells in peripheral blood of the group GD patients was positively correlated with the FT3 and FT4 in the serum (r= 0.682, 0.05), and had no statistical difference from other thyroid function indexes. The SFB content of intestinal microflora in group P0.05.GD was about 2.384 x 103 times (RQ value 2.384 * 103) in group NC, and in group GD, the content of enteric bacteria in intestinal flora was 1.825 x 103 times (1.825 * 103) in NC group (RQ value 1.825 * 103). Conclusion: the ratio of Th17 cells in peripheral blood of patients with 1.Graves disease was significantly higher than that in normal control group, and the FT4 was significantly positive with peripheral blood. The contents of segmented filamentous bacteria and E. coli in 2.Graves patients were significantly higher than those in normal control group.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R581.1

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