本文選題：甲狀腺相關性眼病 + 眼外肌��； 參考：《山西醫(yī)科大學》2015年碩士論文

【摘要】：目的:通過檢測抗肌集鈣蛋白(calsequestrin)抗體、抗葡萄膜自身抗原(uveal antoantigen with coiled coil domains and ankyrin repeats,UACA)抗體、抗G2s抗體3種自身抗體在甲狀腺相關性眼病患者、單純Graves病患者及健康人群血清中的表達水平,初步探討這三種自身抗體在甲狀腺相關性眼病患者發(fā)病中的特點。方法:研究對象分為三組,共90例,其中TAO組為34例(男13例,女21例)、Graves病(GD)組28例(男11例,女17例)和健康對照(CON)組28例(男11例,女17例)。三組研究對象的基本資料經統計學分析,在性別、年齡以及病程間均具備可比性,并簽署了知情同意書。分別取外周抗凝血,在離心后提取血漿,選用雙抗原夾心法(酶聯免疫吸附法),分別測定所有研究對象血清中抗calsequestrin抗體、抗UACA抗體、抗G2s抗體、三類自身抗體的濃度。應用SPSS19.0統計學軟件進行分析,每個指標的濃度值以x±s表示,分別對三組數據進行分析,對符合正態(tài)性且方差齊的資料數據采用方差分析并進行各組間的LSD多重比較方法。對于資料分布類型呈偏態(tài)分布或方差不齊的使用秩和檢驗中的Kruskal-Wallis法及Bonferroni法進行組間的比較。P0.05為差異具有統計學意義。結果:(1)抗calsequestrin抗體在各組研究對象血清中的濃度(ng/L)分別為:TAO組153.0206±104.84868;GD組99.7002±66.42114;CON組88.0589±39.73018;TAO組與CON組比較,P=0.004;TAO組與GD組比較,P=0.016;GD組與CON組比較,P=0.604。(2)抗UACA抗體在各組研究對象血清中的濃度(ng/L)依次為:TAO組60.5365±38.23955;GD組46.6286±17.99325;CON組34.2016±12.59909;TAO組與CON組比較,P=0.000;TAO組與GD組比較,P=0.075;GD組與CON組比較,P=0.002。(3)抗G2s抗體在在各組研究對象血清中的濃度(m U/L)分別為:TAO組68.7604±35.82627;GD組64.9248±31.29806;CON組62.6828±18.40611;TAO組與CON組比較,P=0.478;TAO組與GD組比較,P=0.654;GD組與CON組比較,P=0.799。結論:(1)經ELISA法檢測,抗calsequestrin抗體在TAO患者血清中的濃度明顯高于GD患者和健康人群,結果存在統計學差異(P0.05),抗calsequestrin抗體可以作為由免疫介導的TAO患者眼肌損傷的敏感性指標。(2)抗UACA抗體在TAO患者血清中的濃度水平明顯超出正常人群,結果存有統計學意義(P0.05),抗UACA抗體可以作為自身免疫病的一種臨床標志物,本次實驗未發(fā)現TAO患者血清中抗UACA抗體的表達與GD患者血清中的表達存在差異。(3)本次實驗未發(fā)現在TAO患者血清中抗G2s抗體的濃度與GD患者及健康人群存有差異,尚不能認為抗G2s抗體可以作為與TAO有關的一個標記物。
[Abstract]:Objective: to detect three kinds of autoantibodies against calsequestrin, uveal antoantigen with coiled coil domains and ankyrin repeat UACAand G2s antibodies in patients with thyroid associated ophthalmopathy. To explore the characteristics of the three autoantibodies in the pathogenesis of thyroid associated ophthalmopathy. Methods: the subjects were divided into three groups, including 34 cases (male 13 cases, female 21 cases with Graves disease) group (28 cases male 11 cases, female 17 cases) and healthy control group 28 cases (male 11 cases, female 17 cases). The basic data of the three groups were statistically analyzed and compared among sex, age and course of disease, and informed consent was signed. The plasma was extracted from peripheral anticoagulant blood after centrifugation. The concentrations of anti calsequestrin antibody, anti UACA antibody, anti G 2s antibody and three kinds of autoantibodies were determined by double antigen sandwich assay (Elisa). Three groups of data were analyzed by using SPSS19.0 software, and the concentration of each index was expressed as x 鹵s. The data with normality and homogeneity were analyzed by ANOVA and LSD multiple comparison among each group. For the data distribution type of skewed distribution or variance of the use of rank sum test in the Kruskal-Wallis and Bonferroni methods to compare between groups. P0.05 was statistically significant difference. Results the concentration of calsequestrin antibody in the serum of each group was 153.0206 鹵104.84868 g / L respectively, 99.7002 鹵66.42114Con group 88.0589 鹵39.73018 TAO group compared with GD group and GD group compared with GD group. 渚濇涓，

本文編號：1935673