本文選題：雙峰駝奶 + 2型糖尿病大鼠��； 參考：《甘肅農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：本論文研究了雙峰駝奶對2型糖尿病大鼠肝臟和腎臟組織的保護(hù)作用,旨在進(jìn)一步為利用駝奶有效成分在臨床上預(yù)防和治療糖尿病及其并發(fā)癥提供理論參考。通過鏈脲佐菌素(STZ)結(jié)合高糖高脂飲食誘導(dǎo)構(gòu)建了2型糖尿病(T2DM)大鼠模型,糖尿病模型大鼠隨機(jī)分為糖尿病模型組(DMC)、低劑量駝奶組(LCM)、高劑量駝奶組(HCM)和鹽酸二甲雙胍組(MTH),另設(shè)正常對照組(C)。試驗期4周內(nèi),檢測各組大鼠的生理生化指標(biāo),并采用H.E染色法、免疫組織化學(xué)法、免疫印跡法和實時熒光定量法,探討不同劑量駝奶對大鼠肝臟和腎臟的組織形態(tài)和細(xì)胞凋亡的影響。結(jié)果顯示,各實驗組糖尿病模型大鼠的體重均呈下降趨勢,與DMC組和MTH組相比,LCM組大鼠體重顯著降低(P0.01);給予葡萄糖后的120 min內(nèi),各組大鼠的血糖濃度均先升高后降低,4個時間點的LCM組和HCM組血糖濃度顯著低于DMC組(P0.01),同時,駝奶降低了糖尿病模型大鼠的空腹血糖水平(FPG)和空腹胰島素(FINS)水平,提高胰島素敏感指數(shù)(ISI);經(jīng)過駝奶治療后,肝臟組織部分細(xì)胞恢復(fù)正常結(jié)構(gòu),脂肪變性程度明顯減輕,沒有空泡化現(xiàn)象,腎臟組織基本形態(tài)結(jié)構(gòu)完整清晰,腎小球毛細(xì)血管無明顯擴(kuò)張,囊腔間隙正常,大部分腎小管上皮細(xì)胞形態(tài)結(jié)構(gòu)正常;免疫組化和免疫印跡法表明駝奶可抑制Bax和NF-?B蛋白的表達(dá),上調(diào)Bcl-2蛋白的表達(dá)量;與MTH相比,駝奶可抑制肝臟組織中Bax和NF-?B m RNA的表達(dá)量,上調(diào)了caspase-3 m RNA的表達(dá)量,下調(diào)Bcl-2 m RNA的表達(dá)量,此外,駝奶還降低腎臟組織中Bax和NF-?B m RNA的表達(dá)量,上調(diào)Bcl-2 m RNA的表達(dá)量,而caspase-3 m RNA在LCM和HCM組的高表達(dá)水平,仍待進(jìn)一步研究。綜上所述,駝奶可改善2型糖尿病大鼠的生理生化指標(biāo),抑制肝、腎組織相關(guān)凋亡蛋白和基因的表達(dá)量,結(jié)果提示雙峰駝奶對2型糖尿病大鼠的肝臟和腎臟組織有一定的保護(hù)作用。
[Abstract]:In this paper, the protective effect of bactrian camel milk on liver and kidney tissue of type 2 diabetic rats was studied. The aim of this study was to provide a theoretical reference for the clinical prevention and treatment of diabetes mellitus and its complications by using the effective components of camel milk. A rat model of type 2 diabetes mellitus (T2DM) was established by streptozotocin (STZ) combined with high glucose and high fat diet. Diabetic rats were randomly divided into diabetic model group, low dose camel milk group, high dose camel milk group (HCM) and metformin hydrochloride group, and normal control group. The physiological and biochemical indexes of rats in each group were detected during the four weeks of experiment, and were detected by e staining, immunohistochemistry, immunoblotting and real-time fluorescence quantitative analysis. To investigate the effects of different doses of camel milk on the histomorphology and apoptosis of rat liver and kidney. The results showed that the weight of diabetic rats in each experimental group showed a decreasing trend, and the weight of rats in DMC group was significantly lower than that in DMC group and MTH group, and within 120 min after glucose administration, the weight of rats in LCM group was significantly lower than that in MTH group. The blood glucose levels in the LCM and HCM groups were significantly lower than those in the DMC group at four time points, while the fasting blood glucose levels and fasting insulin fins levels in the diabetic model rats were decreased by camel milk. After treatment with camel milk, some cells of liver returned to normal structure, the degree of steatosis was obviously reduced, there was no vacuolation, and the basic morphological structure of kidney was complete and clear. The expression of Bax and NF-?B protein was inhibited and the expression of Bcl-2 protein was up-regulated by camel milk. Compared with MTH, camel milk inhibited the expression of Bax and NF-?B m RNA, up-regulated the expression of caspase-3 m RNA, down-regulated the expression of Bcl-2 m RNA, and decreased the expression of Bax and NF-?B m RNA in kidney. The upregulation of Bcl-2 m RNA expression and the high expression of caspase-3 m RNA in LCM and HCM groups need further study. In conclusion, camel milk can improve physiological and biochemical indexes and inhibit the expression of apoptotic proteins and genes in liver and kidney tissues of type 2 diabetic rats. The results suggest that bactrian camel milk can protect the liver and kidney of type 2 diabetic rats.
【學(xué)位授予單位】：甘肅農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R587.1

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本文編號：1923949