本文選題：關(guān)節(jié)炎 + 類風(fēng)濕　； 參考：《暨南大學(xué)學(xué)報(bào)(自然科學(xué)與醫(yī)學(xué)版)》2017年01期

【摘要】：目的:探討類風(fēng)濕關(guān)節(jié)炎(RA)患者外周血中輔助性T細(xì)胞22(Th22)/白細(xì)胞介素-22(IL-22)的表達(dá)情況及其與臨床指標(biāo)和癥狀之間的相關(guān)性.方法:流式細(xì)胞術(shù)檢測RA患者(50例)、骨關(guān)節(jié)炎(OA)患者(15例)、健康對照組(15例)外周血中Th22細(xì)胞所占比例,ELISA法檢測血清中IL-22水平.采用t檢驗(yàn)和Pearson相關(guān)分析法進(jìn)行統(tǒng)計(jì)學(xué)分析.結(jié)果:RA患者外周血中Th22細(xì)胞比例[(1.98±0.71)%]高于OA組[(1.21±0.52)%](t=2.290,P=0.021)及健康對照組[(1.18±0.45)%](t=2.524,P=0.015).RA患者血清IL-22水平[(53.746±8.327)ng/L]高于OA患者[(37.918±5.687)ng/L](t=2.560,P=0.014)及健康對照組[(34.638±5.231)ng/L](t=2.768,P=0.009).RA患者外周血Th22細(xì)胞比例與血清IL-22水平、血沉(ESR)、類風(fēng)濕關(guān)節(jié)炎患者病情評價(jià)(DAS28)評分呈正相關(guān)關(guān)系(r分別為0.42、0.38、0.32,P0.05).RA患者抗合并間質(zhì)性肺疾病(CCP)陽性組Th22細(xì)胞比例高于抗CCP陰性組,差異有統(tǒng)計(jì)學(xué)意義(t=2.785,P=0.008).抗CCP陰性RA組Th22細(xì)胞比例與病程呈負(fù)相關(guān)(r=-0.59,P=0.04).RA有骨侵蝕組Th22細(xì)胞比例高于無骨侵蝕組,差異有統(tǒng)計(jì)學(xué)意義(t=2.542,P=0.014).RA-ILD組Th22細(xì)胞比例高于RA患者未合并間質(zhì)性肺疾病(RA-NILD)組,差異有統(tǒng)計(jì)學(xué)意義(t=2.513,P=0.017).結(jié)論:RA患者外周血Th22細(xì)胞及IL-22表達(dá)增高,Th22細(xì)胞比例與IL-22水平及疾病活動(dòng)度(ESR、DAS28評分)正相關(guān),同時(shí)與骨侵蝕和ILD的發(fā)生有相關(guān)性,提示Th22細(xì)胞參與RA發(fā)病機(jī)制,有可能成為RA治療的新靶點(diǎn).Th22細(xì)胞在抗CCP陰性RA中的表達(dá)情況有助于RA的早期診斷.
[Abstract]:Objective: to investigate the expression of 22(Th22)/ interleukin-22 (IL-22) in peripheral blood of patients with rheumatoid arthritis (RA) and its correlation with clinical indexes and symptoms. Methods: the proportion of Th22 cells in peripheral blood of 50 patients with RA, 15 patients with osteoarthritis and 15 healthy controls were detected by flow cytometry. The serum IL-22 levels were detected by Elisa. T test and Pearson correlation analysis were used. 緇撴灉:RA鎮(zhèn)ｈ，

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