本文選題：Wnt信號通路 + 百草枯��； 參考：《醫(yī)學研究生學報》2017年03期

【摘要】：目的百草枯中毒患者可因不可逆的肺纖維化而死亡,而Wnt信號通路參與了肺纖維化的發(fā)生發(fā)展。文中通過建立百草枯中毒大鼠模型,探討Wnt信號通路在百草枯中毒大鼠肺纖維化中的作用。方法選用健康雄性SD大鼠36只,隨機數(shù)字表法分為對照組、百草枯中毒組和抑制劑組,每組12只。百草枯中毒組大鼠采用一次性腹腔注射百草枯20 mg/kg建立百草枯中毒大鼠肺纖維化模型,對照組給予腹腔注射等量等滲鹽水,抑制劑組采用一次性腹腔注射百草枯20 mg/kg同時經(jīng)氣道給予Wnt信號通路抑制劑DKK1(2 mg/kg,100μL PBS)。3組均第28天處死大鼠,取出肺組織常規(guī)進行HE染色和馬松染色,利用Ray Bio蛋白芯片技術檢測對照組和百草枯中毒組相關蛋白表達情況,利用Western blot技術檢測各組Wnt信號通路關鍵蛋白β-catenin和MMP-2的表達。結(jié)果百草枯中毒組HE染色和馬松染色結(jié)果示:肺泡結(jié)構(gòu)紊亂,炎癥細胞浸潤,膠原纖維沉積,抑制劑組較百草枯中毒組肺纖維化程度明顯減輕,膠原蛋白沉積減少,而對照組肺泡結(jié)構(gòu)清晰,無膠原纖維沉積。Ray Bio蛋白芯片技術結(jié)果示百草枯中毒組肺組織IL-1β、PDGF、β-catenin、MMP-2和TGF-β1表達水平異常升高。與對照組β-catenin、MMP(0.31±0.03,0.28±0.07)比較,百草枯中毒組(0.82±0.09,1.04±0.08)升高,抑制劑組(0.56±0.06,0.63±0.05)降低(P0.05)。與百草組中毒組比較,抑制劑組β-catenin、MMP表達均降低(P0.05)。結(jié)論百草枯可誘導大鼠發(fā)生肺纖維化,導致Wnt信號通路高度激活,通過抑制Wnt信號通路能夠減弱肺纖維化的程度。
[Abstract]:Objective: paraquat poisoning patients may die of irreversible pulmonary fibrosis, and the Wnt signaling pathway is involved in the development of pulmonary fibrosis. The role of Wnt signaling pathway in pulmonary fibrosis of paraquat poisoned rats was studied by establishing a paraquat poisoning model. Methods Thirty-six male Sprague-Dawley rats were randomly divided into control group, paraquat poisoning group and inhibitor group with 12 rats in each group. The pulmonary fibrosis model of paraquat poisoned rats was established by intraperitoneal injection of paraquat for 20 mg/kg in the paraquat poisoning group, and isosmotic saline was injected intraperitoneally in the control group. In the inhibitor group, paraquat was injected intraperitoneally for 20 mg/kg and Wnt signaling pathway inhibitor DKK1(2 mg / kg ~ (100 渭 L) PBS).3 was administered through the airway. The rats in the control group were killed on the 28th day. The lung tissues were taken out for HE staining and Ma Song staining. The expression of related proteins in control group and paraquat poisoning group was detected by Ray Bio protein chip technique, and the expression of 尾 -catenin and MMP-2 in Wnt signaling pathway was detected by Western blot technique. Results the results of HE staining and Ma Song staining in paraquat poisoning group showed that pulmonary alveolar structure disorder, inflammatory cell infiltration, collagen fiber deposition, pulmonary fibrosis and collagen deposition in inhibitor group were significantly less than those in paraquat poisoning group. In the control group, the alveolar structure was clear and the expression levels of IL-1 尾 -PDGF, 尾 -cateninine MMP-2 and TGF- 尾 1 in the lung tissue of paraquat poisoning group were increased abnormally without collagen deposition. Compared with the control group, 尾 -cateninine (MMPN) 0.31 鹵0.03 (0.28 鹵0.07), paraquat poisoning (0.82 鹵0.09) 1.04 鹵0.08) and inhibitor group (0.56 鹵0.06 鹵0.63 鹵0.05) decreased P0.05N. Compared with the control group, the expression of 尾 -cateninine MMP in the inhibitor group was significantly lower than that in the control group. Conclusion Paraquat can induce pulmonary fibrosis in rats, which leads to high activation of Wnt signaling pathway, which can reduce the degree of pulmonary fibrosis by inhibiting Wnt signaling pathway.
【作者單位】： 南方醫(yī)科大學金陵醫(yī)院(南京軍區(qū)南京總醫(yī)院)急救醫(yī)學科;
【基金】：國家自然科學基金(81401583) 北京協(xié)和醫(yī)學基金會睿E(睿意)急診醫(yī)學科研基金(R2014004) 全軍后勤面上項目(CNJ14L002) 南京軍區(qū)南京總醫(yī)院科研基金(2015027)
【分類號】：R595.4

【參考文獻】

相關期刊論文 前4條

1 張志堅;吳燦;田黎;首云峰;彭禮波;;沙苑子總黃酮對內(nèi)質(zhì)網(wǎng)應激誘導的細胞凋亡在百草枯致大鼠肺損傷中的保護作用[J];醫(yī)學研究生學報;2014年08期

2 錢潔;陸曉曄;朱長清;呂利雄;;水楊酸鈉對急性百草枯中毒大鼠肺損傷的作用研究[J];醫(yī)學研究生學報;2014年02期

3 李偉;張煒;楊敬輝;胡宗風;孫海晨;聶時南;唐文杰;;ICR小鼠百草枯中毒急性肺損傷的模型研究[J];醫(yī)學研究生學報;2013年12期

4 申永春;萬春;文富強;;Wnt/β-catenin信號通路在氣道炎癥性疾病中的研究進展[J];臨床肺科雜志;2013年02期

【共引文獻】

相關期刊論文 前10條

1 黃晶;孫兆瑞;楊志洲;陳鑫;黃昌保;汪林;顧旭東;劉紅梅;張煒;聶時南;;調(diào)控Wnt信號通路對百草枯中毒大鼠肺纖維化的影響[J];醫(yī)學研究生學報;2017年03期

2 李旭;陳巖;白雪松;齊文成;李長輝;林萍;隋吉峰;原藝;;中藥治療急性肺損傷/急性呼吸窘迫綜合征作用機制研究進展[J];遼寧中醫(yī)藥大學學報;2017年01期

3 王曄;曲艷;陳美瓊;郝麗;;5-Aza-2'-dc預處理致敏百草枯對V79細胞活性氧及Bcl-2/Bax表達的影響[J];昆明醫(yī)科大學學報;2016年10期

4 陳未平;房曉薇;吳利東;;依達拉奉聯(lián)合烏司他丁對急性百草枯中毒大鼠肺損傷的保護作用[J];南昌大學學報(醫(yī)學版);2016年04期

5 芮敏R，

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