本文選題：多囊卵巢綜合征 + Nesfatin-1��； 參考：《濱州醫(yī)學(xué)院》2015年碩士論文

【摘要】：目的：探討不同濃度Nesfatin-1對體外培養(yǎng)人卵泡黃素化顆粒細(xì)胞分泌雌激素(E2)、孕激素(P)的影響。方法：篩選20例在濱州醫(yī)學(xué)院附屬醫(yī)院生殖醫(yī)學(xué)科接受體外受精-胚胎移植(IVF-ET)的不孕患者,分成PCOS組20組和對照組15組,采用超促排卵方案,取卵日收集成熟卵母細(xì)胞周圍的卵丘顆粒細(xì)胞,加入含血清培養(yǎng)液(DMEM+10%胎牛血清)體外培養(yǎng)卵泡黃素化顆粒細(xì)胞48h后傳代,顆粒細(xì)胞計數(shù)并調(diào)整細(xì)胞濃度,按2X10S/ml密度接種于24孔培養(yǎng)板,加入不同濃度(1X10-7M、1X10-9 M、1X10-11 M、0)Nesfatin-1的無血清培養(yǎng)液(DMEM)培養(yǎng)24h,收集上清液至-80℃冰箱凍存。采用化學(xué)發(fā)光法測定顆粒細(xì)胞培養(yǎng)液中E2、P的含量。結(jié)果：1、體外培養(yǎng)黃素化顆粒細(xì)胞加入不同濃度的(1X10-7M、1X10-9M、1X10-11M、0)Nesfatin-1共培養(yǎng)24h后,我們發(fā)現(xiàn)：對照組黃素化顆粒細(xì)胞生成E2水平隨Nesfatin-1濃度增加有增加趨勢,各組間有統(tǒng)計學(xué)差異。同樣PCOS組黃素化顆粒細(xì)胞生成E2水平隨Nesfatin-1濃度增加而增加。且在不同Nesfatin-1濃度中,PCOS組黃素化顆粒細(xì)胞E2生成水平均明顯低于對照組,差異具有統(tǒng)計學(xué)意義(P0.05)。2、體外培養(yǎng)黃素化顆粒細(xì)胞加入不同濃度的(1X10-7M、1X10-9M、1X10-11M、0)Nesfatin-1共培養(yǎng)24h后,我們發(fā)現(xiàn)：PCOS組黃素化顆粒細(xì)胞生成P水平加入Nesfatin-1后與基礎(chǔ)狀態(tài)比較明顯增加,差異有統(tǒng)計學(xué)意義(P0.05),對照組黃素化顆粒細(xì)胞生成P水平隨Nesfatin-1濃度增加與基礎(chǔ)狀態(tài)比較差異均無有統(tǒng)計學(xué)意義(P0.05)。在不同Nesfatin-1濃度中,對照組黃素化顆粒細(xì)胞生成P水平均明顯高于PCOS組,差異具有統(tǒng)計學(xué)意義(P0.05)。結(jié)論：Nesfatin-1能促進(jìn)人卵泡黃素化顆粒細(xì)胞分泌雌二醇,且促進(jìn)作用隨Nesfatin-1濃度的增加有增加趨勢；對PCOS組孕酮的分泌有促進(jìn)作用,對正常組P分泌無明顯影響,這可能與顆粒細(xì)胞所處時期及是否有FSH存在有關(guān)。
[Abstract]:Aim: to investigate the effects of different concentrations of Nesfatin-1 on the secretion of estradiol E2 and progesterone by human follicular luteinized granulosa cells in vitro. Methods: twenty infertile patients who received IVF-ETT in the Department of Reproductive Medicine, affiliated Hospital of Binzhou Medical College were selected and divided into two groups: PCOS group (n = 20) and control group (n = 15). The cumulus granulosa cells around the mature oocytes were collected on the day of oocyte extraction. The follicular luteinized granulosa cells were cultured in vitro with DMEM 10% fetal bovine serum. The granulosa cells were counted and the cell concentration was adjusted. According to the density of 2X10S/ml, we inoculated on 24-well culture plate and added different concentrations of 1X10-7MnlX10-9 M10-9 MX10-11 M10-11 Nesfatin-1 serum-free medium for 24 hours. The supernatant was collected and stored in refrigerator at -80 鈩，

本文編號：1808925