本文選題：糖尿病 + 甲亢　； 參考：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2016年11期

【摘要】：目的:評估糖尿病和甲狀腺功能亢進(jìn)對雄性動物睪丸和附睪組織形態(tài)學(xué)及類固醇激素合成的影響,并初步探討其作用機(jī)制。創(chuàng)新點:以小鼠為模型,首次研究并發(fā)糖尿病和甲狀腺功能亢進(jìn)對雄性哺乳動物睪丸、附睪發(fā)育和類固醇激素合成的影響。方法:32只ICR品系小鼠分為四組:對照組(C)、糖尿病組(D)、糖尿病+甲亢組(DH)和甲亢組(H)。D組小鼠以200 mg/kg劑量單次腹膜內(nèi)注射鏈脲佐菌素(STZ),誘導(dǎo)糖尿病成功。另對其中一半以0.3 mg/kg劑量每天注射甲狀腺素,組成DH組。小鼠試驗結(jié)束后,采集睪丸、附睪和血液,并離心分離獲得血清。睪丸和附睪用4%(0.04 g/ml)多聚甲醛固定,并用蘇木精-伊紅染色法(HE)觀察睪丸和附睪組織形態(tài)學(xué)變化,用放射免疫測定(RIA)試劑盒檢測血清中睪酮、促甲狀腺激素(TSH)、胰島素、甲狀腺素(T4)和三碘甲狀腺原氨酸(T3)的含量并進(jìn)行分析。結(jié)論:D和DH組小鼠的體重、睪丸和附睪的重量顯著降低。相比于正常甲亢或糖尿病小鼠,DH組中血糖水平顯著升高。甲狀腺激素可能是通過改變糖尿病患者的血清血糖水平對血糖穩(wěn)態(tài)產(chǎn)生瞬時影響。組織形態(tài)學(xué)分析結(jié)果顯示,在DH和H組小鼠睪丸中,輸精管管腔增大,上皮厚度減少,睪丸生殖干細(xì)胞發(fā)生萎縮性變化。DH組小鼠的附睪頭呈現(xiàn)主細(xì)胞壓實、纖毛、脂質(zhì)空泡化和炎癥浸潤現(xiàn)象。在附睪尾部觀察到了小管完整性受損、透明細(xì)胞聚積和細(xì)胞脫落,并發(fā)現(xiàn)圓形精子。對于DH和H組,甲亢提高了小鼠血清睪酮水平,并損害了附睪的組織形態(tài)。總之,本試驗?zāi)M了多腺體自身免疫綜合征對雄性繁殖的影響,這將有助于更好地了解男性并發(fā)糖尿病和甲亢患者不育的原因。
[Abstract]:Aim: to evaluate the effects of diabetes mellitus and hyperthyroidism on testicular and epididymal histomorphology and steroid hormone synthesis in male animals and to explore its mechanism. Innovation: the effects of diabetes mellitus and hyperthyroidism on testicular, epididymal development and steroid hormone synthesis in male mammals were studied for the first time in mice. Methods Thirty-two ICR mice were divided into four groups: control group, diabetes group, hyperthyroidism group and hyperthyroidism group. Mice in hyperthyroidism group were treated with intraperitoneal injection of streptozotocin (STZ) at a single dose of 200 mg/kg to induce diabetic success. The other half were injected with thyroxine at a dose of 0.3 mg/kg daily to form DH group. After the experiment, the testis, epididymis and blood were collected, and the serum was isolated by centrifugation. The testis and epididymis were fixed with 0.04 g / ml of paraformaldehyde. The morphological changes of testis and epididymis were observed by hematoxylin and eosin staining. Serum testosterone, TSH, insulin were detected by radioimmunoassay (RIA) kit. The contents of thyroxine T _ 4) and triiodothyronine T _ 3) were analyzed. Conclusion the body weight, testis and epididymis weight of mice in the groups of W D and DH were significantly decreased. Compared with normal hyperthyroidism or diabetic mice, the blood glucose level in DH group was significantly higher than that in normal hyperthyroidism or diabetic mice. Thyroid hormone may have a transient effect on glucose homeostasis by changing serum glucose levels in diabetics. Histomorphologic analysis showed that in the testis of DH and H groups, the vas deferens were enlarged, the thickness of epithelium decreased, and the atrophic changes of testicular reproductive stem cells. The epididymal head of the DH group showed compaction and cilium. Lipid vacuolation and inflammatory infiltration. Tubule integrity was impaired, clear cell accumulation and cell shedding were observed in the caudal epididymis, and round sperm was found. In DH and H groups, hyperthyroidism increased serum testosterone levels and damaged the histomorphology of epididymis. In conclusion, this study simulates the effect of polyglandular autoimmune syndrome on male reproduction, which will contribute to a better understanding of the causes of male infertility in patients with diabetes and hyperthyroidism.
【作者單位】： Laboratory
【基金】：Project supported by the National Natural Science Foundation of China(Nos.31572403,31172206,and 31402075)
【分類號】：R587.1;R581.1

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