本文選題：鞣花酸 + 肥胖��； 參考：《新疆醫(yī)科大學》2017年碩士論文

【摘要】：目的:通過鞣花酸干預單一的高脂飲食誘導的大鼠肥胖,探討其對肥胖的影響及作用。方法:健康SPF級雄性SD大鼠56只按體重隨機分為七組,對照組,模型組,鞣花酸高劑量組(30mg/kg),鞣花酸低劑量組(10mg/kg),鹽酸二甲雙胍緩釋片陽性對照藥(89.25 mg/kg),奧利司他陽性對照藥(37.8 mg/kg)和硫普羅寧腸溶片陽性對照藥(47.25 mg/kg),每周測量一次體重,兩次進食量,分別在第12周和第25周量取大鼠體長。第25周時,分別腹腔注射葡萄糖溶液和胰島素溶液,檢測血糖水平;用超聲診斷儀測量腎橫切面中部前方脂肪層厚度;處理動物取血檢測HDL-C、LDL-C、TC和TG水平;取附睪周和腎周白色脂肪組織和肩胛處棕色脂肪組織進行HE染色觀察脂肪細胞形態(tài),并計算白色脂肪系數(shù);取肝臟計算肝臟系數(shù)后HE染色進行細胞形態(tài)觀察;Western blot法檢測附睪周白色脂肪組織中PPAR γ、C/EBP-β、C/EBP-α、UCP-1和PGC-1α蛋白的表達水平。結果:1.鞣花酸高劑量組(417.06±42.93),鞣花酸低劑量組(395.61±30.83),鹽酸二甲雙胍緩釋片組(412.07±49.26),奧利司他組(408.09±52.18)和硫普羅寧腸溶片組(395.93±63.43)較模型組(559.45±59.46)大鼠體重均明顯降低(p0.05),但組間進食量無差異(p0.05);2.腹腔注射葡萄糖溶液后,與模型組(158.63 ±6.00,13982.63±933.17)比較,鞣花酸高(127.13 ±27.82,12423.38 ± 1299.97)、低劑量組(121.63 ±7.84,12412.29 ± 872.91)30 min時的血糖水平以及曲線下面積均顯著降低(p0.05);腹腔注射胰島素溶液后,鞣花酸高(2.59 ±0.16,439.50 ±22.65)、低劑量組(3.21 ±0.22,457.81 ±27.02)30 min時的血糖水平以及曲線下面積較模型組(4.65 ±0.35,503.81 ±26.26)亦均顯著降低(p0.05);3.鞣花酸高(3.97±0.42)、低劑量組(3.51±0.38)HDL-C 水平較模型組(2.9±0.59)均顯著升高(p0.05);4.與模型組(0.22±0.03)比較,鞣花酸高(0.05±0.01)、低劑量組(0.06±0.03)腎周脂肪層厚度明顯減少(p0.05);高(1.96±0.16)、低劑量組(2.08±0.13)的白色脂肪系數(shù)較模型組(4.26±0.19)也明顯降低(p0.05)。HE結果顯示,與模型組比較,鞣花酸干預后,附睪周白色脂肪細胞體積明顯變小且數(shù)量顯著減少,肩胛處棕色脂肪細胞體積變小且組織內毛細血管量增加,肝臟內空泡明顯減少,肝臟細胞排列趨于規(guī)則。5.鞣花酸高劑量組大鼠附睪周脂肪組織中PPAR γ(0.22±0.01)、C/EBP-β(0.25±0.09)和 C/EBP-α(0.20±0.06)蛋白表達量較模型組(0.44±0.06,0.45±0.04,0.50±0.09)均顯著降低(p0.05);而 UCP-1(0.38±0.05)和 PGC-1α(0.43±0.08)蛋白表達量較模型組(0.18±0.04,0.16±0.06)則顯著增加(p0.05)。結論:鞣花酸高、低劑量均具有良好的降糖作用,可以改善胰島素抵抗,減少體內白色脂肪的積累,鞣花酸高劑量降低脂質合成關鍵蛋白表達的同時增加棕色脂肪標志蛋白的表達,可能具有促使體內白色脂肪組織棕色樣變的作用,和預防高脂飲食誘導的肥胖的形成。
[Abstract]:Aim: to investigate the effect of tannic acid on obesity induced by high fat diet in rats. Methods: Fifty-six male SD rats of SPF grade were randomly divided into seven groups according to their body weight: control group, model group, and control group. The high dose of tannic acid was 30 mg / kg / kg, the low dose group of tannin was 10 mg / kg / kg, the metformin hydrochloride sustained release tablet was 89.25 mg / kg / kg, the orlistat positive control drug was 37.8 mg / kg) and the tiopronin enteric-coated drug was 47.25 mg / kg 路kg ~ (-1). The body length of rats was taken at the 12th week and the 25th week, respectively. At the 25th week, glucose solution and insulin solution were injected intraperitoneally to detect the blood glucose level, the thickness of the anterior adipose layer in the middle of the cross section of kidney was measured by ultrasonic diagnostic instrument, and the HDL-CU LDL-CU TC and TG levels were measured by blood collection from the treated animals. The white adipose tissue around epididymis and kidney and brown adipose tissue of scapula were stained with HE to observe the morphology of adipocytes and calculate the coefficient of white fat. The expression levels of PPAR 緯 C / EBP- 尾 C / EBP- 偽 UCP-1 and PGC-1 偽 in white adipose tissue around epididymis were detected by using HE staining after liver coefficient was calculated. The result is 1: 1. The rats in the high dose group of tannic acid 417.06 鹵42.93%, the low dose group of tannic acid 395.61 鹵30.83L, the metformin hydrochloride sustained-release tablet group 412.07 鹵49.26, the olesta group 408.09 鹵52.18) and the tiopronin enteric-coated tablet group 395.93 鹵63.43) the body weight of the rats decreased significantly compared with the model group (559.45 鹵59.46), but there was no difference in food intake between the two groups. After intraperitoneal injection of glucose solution, compared with model group (158.63 鹵6.00 鹵13982.63 鹵933.17), the level of blood glucose and the area under the curve decreased significantly at 121.63 鹵7.8412.29 鹵872.9130 min in the low dose group and 127.13 鹵27.82 鹵12423.38 鹵1299.97 in the low dose group, respectively, and the area under the curve decreased significantly after intraperitoneal injection of insulin solution. The blood glucose level and area under the curve of the low dose group (3.21 鹵0.22457.81 鹵27.02U 30 min) were significantly lower than that of the model group (4.65 鹵0.35503.81 鹵26.26). The blood glucose level of the low dose group was also significantly lower than that of the model group (4.65 鹵0.35503.81 鹵26.26). The level of 0.38)HDL-C in low dose group (3.51 鹵0.59) was significantly higher than that in model group (3. 97 鹵0. 42) (P < 0. 05). Compared with the model group (0.22 鹵0.03), the thickness of perirenal adipose layer decreased significantly in the tannic acid group (0.05 鹵0.01g) and the low-dose group (0.06 鹵0.03), and the white fat coefficient of the high-dose group (1.96 鹵0.16) was significantly lower than that of the model group (4.26 鹵0.19). The results showed that compared with the model group, the white fat coefficient of the model group was significantly lower than that of the model group. The volume of white adipocytes around epididymis became smaller and the number of white adipocytes decreased significantly, the volume of brown adipocytes in scapula became smaller and the amount of capillaries in tissues increased, the vacuoles in liver decreased obviously, and the arrangement of liver cells tended to be regular. 5. Compared with model group, the expression levels of PPAR 緯 -0.22 鹵0.01C / EBP- 尾 0.25 鹵0.09) and C / EBP- 偽 0.20 鹵0.06) in the high dose group were significantly lower than those in the model group (0.44 鹵0.06 鹵0.04 鹵0.04 鹵0.50 鹵0.09), while the expression of UCP-1(0.38 鹵0.05) and PGC-1 偽 (0.43 鹵0.08) were significantly higher than those in the model group (0.18 鹵0.04 鹵0.16 鹵0.06). Conclusion: both high and low doses of tannic acid have good hypoglycemic effect, which can improve insulin resistance and reduce the accumulation of white fat in body. The high dose of tannic acid can decrease the expression of key protein of lipid synthesis and increase the expression of brown fat marker protein, which may promote the brown appearance of white adipose tissue in vivo and prevent the formation of obesity induced by high fat diet.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R589.2

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