本文選題：類風(fēng)濕關(guān)節(jié)炎 + 樹(shù)突狀細(xì)胞�。� 參考：《桂林醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:研究腫瘤壞死因子受體(TNFR)相關(guān)因子6(TRAF6)與樹(shù)突狀細(xì)胞(dendritic cells,DCs)成熟性的關(guān)系。方法:建立膠原誘導(dǎo)性關(guān)節(jié)炎(CIA)小鼠模型,從小鼠股骨中提取骨髓細(xì)胞。重組小鼠粒細(xì)胞-巨噬細(xì)胞集落刺激因子(rmGM-CSF)、重組小鼠白細(xì)胞介素-4(rmIL-4)誘導(dǎo)CIA小鼠骨髓細(xì)胞定向分化為未成熟樹(shù)突狀細(xì)胞(immature dendritic cell,iDCs),將iDCs分為針對(duì)TRAF6的小干擾RNA(TRAF6-siRNA)轉(zhuǎn)染組、陰性siRNA轉(zhuǎn)染組和未轉(zhuǎn)染組。轉(zhuǎn)染后實(shí)時(shí)熒光定量PCR檢測(cè)TRAF6基因表達(dá)。脂多糖刺激si RNA轉(zhuǎn)染后的iDCs成熟,流式細(xì)胞術(shù)檢測(cè)DCs成熟程度,酶聯(lián)免疫吸附試驗(yàn)檢測(cè)DCs釋放的促炎癥性因子。結(jié)果:CIA小鼠造模成功率達(dá)84%。轉(zhuǎn)染TRAF6-siRNA組iDCs TRAF6 mRNA表達(dá)明顯低于陰性siRNA轉(zhuǎn)染組及未轉(zhuǎn)染組(P0.05),CD80、CD86和MHC-Ⅱ表達(dá)明顯低于陰性siRNA轉(zhuǎn)染組及未轉(zhuǎn)染組(P0.05),IL-12和TNF-α表達(dá)明顯低于陰性siRNA轉(zhuǎn)染組及轉(zhuǎn)染組(P0.05)。結(jié)論:干擾TRAF6因子在iDCs中表達(dá)可抑制DCs的成熟和促炎癥因子的釋放,TRAF6在RA免疫耐受中起到重要作用。
[Abstract]:Aim: to study the relationship between tumor necrosis factor receptor (TNFR) related factor 6 (TRAF6) and dendritic cells (DCs) maturation. Methods: the collagen induced arthritis (CIA) mouse model was established and bone marrow cells were extracted from the femur of the mice. Recombinant mouse granulocyte-macrophage colony stimulating factor (rmGM-CSFG) and recombinant mouse interleukin-4 (rmIL-4) were used to induce CIA mouse bone marrow cells to differentiate into immature dendritic cells (immature dendritic cells). IDCs was divided into small interfering RNAs (TRAF6-siRNAs) transfection group. Negative siRNA transfection group and non-transfection group. The expression of TRAF6 gene was detected by real-time fluorescence quantitative PCR after transfection. The iDCs matured by lipopolysaccharide stimulated si RNA transfection, the degree of DCs maturation was detected by flow cytometry, and the pro-inflammatory factor released by DCs was detected by enzyme-linked immunosorbent assay (Elisa). Results the success rate of mouse model was 84%. The expression of iDCs TRAF6 mRNA in the TRAF6-siRNA group was significantly lower than that in the negative siRNA transfection group and the untransfected siRNA group. The expression of CD86 and MHC- 鈪，

本文編號(hào)：1787035