本文選題：骨質(zhì)疏松 + 轉(zhuǎn)錄因子��； 參考：《中國組織工程研究》2016年07期

【摘要】：背景:成骨細胞主要來自于骨髓細胞向骨基質(zhì)中的間充質(zhì)細胞,一些轉(zhuǎn)錄因子或局部因素可促進骨髓基質(zhì)細胞調(diào)節(jié)分化為成骨細胞。目的:明確C_2C_12細胞以及Osx與Satb2在骨質(zhì)疏松修復(fù)過程中的作用。方法:取野生型SD大鼠20只,分為正常組10只,假手術(shù)組和骨質(zhì)疏松模型組(模型組)各5只,同時取Osx-KO大鼠10只。模型組和Osx-KO大鼠切除雙側(cè)卵巢構(gòu)建野生型大鼠與Osx-KO大鼠進行骨質(zhì)疏松模型;假手術(shù)組找出雙側(cè)卵巢但不切除。檢測各組大鼠術(shù)后體質(zhì)量的變化及股骨骨密度含量。體外培養(yǎng)C_2C_12細胞,并設(shè)計了si RNA-Satb2、si RNA-Osx,通過細胞實驗、基因沉默、western blot法,觀察成骨細胞分化的相關(guān)Osx與Satb2的表達及對骨質(zhì)疏松的影響。結(jié)果與結(jié)論:(1)體質(zhì)量:造模12周后,模型組、Osx-KO組大鼠較正常組和假手術(shù)組大鼠顯著增加(P0.01)。(2)骨密度:模型組、Osx-KO組較正常組和假手術(shù)組顯著降低(P0.01)。(3)轉(zhuǎn)錄因子:野生型大鼠各因子均有表達,在Osx-KO大鼠中,Satb2、Osx基因的表達顯著降低(P0.001),而Runx2基因在兩種類型的大鼠中的表達差異無顯著性意義。(4)當對基因進行沉默后:再檢測相關(guān)的成骨基因發(fā)現(xiàn)Satb2、Osx、Runx2、ALP基因的表達水平均有顯著的抑制。(5)結(jié)果說明:在骨質(zhì)疏松發(fā)病中轉(zhuǎn)錄因子Osx、Satb2可能是保護性分子,對成骨細胞分化、骨形成與修復(fù)有著關(guān)鍵的調(diào)控作用。
[Abstract]:Background: osteoblasts mainly come from bone marrow cells to mesenchymal cells in bone matrix. Some transcription factors or local factors can promote the regulation and differentiation of bone marrow stromal cells into osteoblasts. Objective: to investigate the role of C_2C_12 cells and Osx and Satb2 in the repair of osteoporosis. Methods: twenty wild Sprague-Dawley rats were divided into normal group (n = 10), sham operation group (n = 5) and osteoporosis model group (n = 5), and Osx-KO rats (n = 10). Osteoporosis model of wild type rats and Osx-KO rats was established in the model group and Osx-KO rats by bilateral ovariectomy, while in the sham-operation group, bilateral ovaries were found out but not resected. The changes of body mass and bone mineral density of femur were measured. C_2C_12 cells were cultured in vitro, and si RNA-Satb2 siRNA-Osx was designed. The expression of Osx and Satb2 in osteoblast differentiation and the effect on osteoporosis were observed by cell experiment and gene silencing western blot method. Results and conclusion: body mass: 12 weeks later, The bone mineral density (BMD) of the model group was significantly higher than that of the normal group and the sham-operated group. The expression of all the factors in the wild type rats was significantly lower than that in the model group compared with the normal group and the sham operation group, and that in the model group was significantly lower than that in the normal group and the sham-operated group, and that in the model group was significantly lower than that in the control group and the sham-operated group. In Osx-KO rats, the expression of Satb2Osx gene decreased significantly, while the expression of Runx2 gene in two types of rats showed no significant difference. The results showed that the transcriptional factor OsxanSatb2 may be a protective molecule in the pathogenesis of osteoporosis. It plays a key role in the regulation of osteoblast differentiation, bone formation and repair.
【作者單位】： 廣州中醫(yī)藥大學;廣西中醫(yī)藥大學附屬第三醫(yī)院;廣東省中醫(yī)院;
【分類號】：R580

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