本文選題：燃煤型氟中毒 + 低蛋白��； 參考：《環(huán)境與健康雜志》2017年07期

【摘要】：為了解低蛋白條件對燃煤型氟中毒雌鼠卵巢顆粒細胞的影響,選取120只斷乳2周的60~80 g清潔級SD雌性大鼠,隨機分為對照組、低蛋白組、染氟組、低蛋白染氟組,每組30只。對照組和低蛋白組進食75%非病區(qū)玉米飼料(含氟量為5.2 mg/kg),染氟組和低蛋白染氟組進食75%燃煤型氟中毒病區(qū)玉米飼料(含氟量為96 mg/kg);對照組及染氟組飼料中大豆蛋白質(zhì)含量為18 g/kg,低蛋白組和低蛋白染氟組為9 g/kg。每組分別于60、120、180 d動態(tài)觀察雌鼠氟斑牙變化情況,測定尿氟、骨氟水平,采用透射電鏡觀察卵巢顆粒細胞超微結(jié)構(gòu)變化,以末端轉(zhuǎn)移酶標(biāo)記技術(shù)(TUNEL法)檢測卵巢顆粒細胞凋亡情況。結(jié)果顯示,成功建立燃煤型氟中毒雌鼠模型,低蛋白染氟組較染氟組電鏡下病理損傷更明顯。與對照組比較,低蛋白組120、180 d卵巢顆粒細胞凋亡率升高,染氟組和低蛋白染氟組60、120、180 d卵巢顆粒細胞凋亡率升高,差異均有統(tǒng)計學(xué)意義(P0.05);低蛋白染氟組60、120、180 d卵巢顆粒細胞凋亡率高于低蛋白組,60、120 d卵巢顆粒細胞凋亡率高于染氟組,差異均有統(tǒng)計學(xué)意義(P0.05)。染氟組180 d時卵巢顆粒細胞凋亡率高于60 d,差異有統(tǒng)計學(xué)意義(P0.05)。析因分析結(jié)果顯示,飼養(yǎng)60、120、180 d時氟對雌鼠卵巢顆粒細胞凋亡率有影響(F值分別為1 180.051,1 187.294,999.577,P0.05);飼養(yǎng)60、120 d時低蛋白對雌鼠卵巢顆粒細胞凋亡率有影響(F值分別為17.613,12.706,P0.05),而飼養(yǎng)180 d時無影響(F=4.017,P0.05),氟與低蛋白對雌鼠卵巢顆粒細胞凋亡率的影響無交互效應(yīng)(F值分別為3.479,0.088,0.941,P0.05)。提示低蛋白條件下燃煤型氟中毒雌鼠卵巢顆粒細胞病理損傷有所加重,但低蛋白與氟染毒對凋亡率的影響無明顯相關(guān)性。
[Abstract]:In order to understand the effect of low protein condition on ovarian granulosa cells of female rats with coal-burning fluorosis, 120 female SD rats of clean grade (6080 g) were randomly divided into control group, low protein group, fluoride group and low protein fluoride group with 30 rats in each group.The control group and the low protein group ate 75% corn feed (5.2 mg 路kg ~ (-1) 路kg ~ (-1)) fluorine, 75% corn feed (96 mg 路kg ~ (-1) 路kg ~ (-1)) of fluorine in the control group and low protein fluoride group, and soybean eggs in the diet of the control group and the fluoride group.White matter content was 18 g / kg, low protein group and low protein fluoride group were 9 g / kg.The changes of fluorosis teeth, urine fluorine and bone fluorine levels in each group were observed at 60120180 days. The ultrastructural changes of ovarian granulosa cells were observed by transmission electron microscope (TEM).The apoptosis of ovarian granulosa cells was detected by terminal transferase labeling technique (Tunel).The results showed that the female model of coal-burning fluorosis was successfully established, and the pathological damage was more obvious in low protein fluoride group than in fluorine-exposed group.Compared with the control group, the apoptosis rate of ovarian granulosa cells in low protein group was higher than that in low protein group at 120180 days, and the apoptosis rate of ovarian granulosa cell in fluoride exposed group and low protein fluoride group at 60120180 days.The apoptosis rate of ovarian granulosa cells in low protein fluoride group was higher than that in low protein group at 60120180 days, and the difference was statistically significant (P 0.05).The apoptosis rate of ovarian granulosa cells in fluoride group was higher than that in 60 days after exposure to fluoride for 180 days, and the difference was statistically significant (P 0.05).The results of factorial analysis show that,After feeding for 60120180 days, F values of F were 1 180.051U 1 187.294999.577G P0.05, F values of low protein on ovarian granulosa cells apoptosis were 17.6131312706P0.05, but no effect on F4.017U P0.05a, F and F on 60120 days, respectively, no effect was observed on the apoptosis rate of ovarian granulosa cells in female rats after feeding for 60120180 days, while F value was not affected at 180 days after feeding, and the effect of F and F on the apoptosis rate of ovarian granulosa cells in female rats were 17.61313 ~ 12.706% P0.05, respectively.The effect of low protein on the apoptosis rate of ovarian granulosa cells in female rats was not interactive. The F value was 3.479 ~ 0.080.88 ~ 0.941% (P = 0.05), respectively.The results suggested that the pathological damage of ovarian granulosa cells was aggravated under low protein condition, but there was no significant correlation between low protein and fluoride exposure on apoptosis rate of female ovarian granulosa cells.
【作者單位】： 貴州醫(yī)科大學(xué)檢驗學(xué)院;貴州醫(yī)科大學(xué)附屬醫(yī)院中心實驗室;貴陽市第二人民醫(yī)院胸外科;上海市閘北區(qū)中心醫(yī)院檢驗科;貴州醫(yī)科大學(xué)公共衛(wèi)生學(xué)院;
【基金】：貴州省科技廳項目(黔科合LG字2016[7242];黔科合LG字[2011036];黔科合J字[20112277]) 國家自然科學(xué)基金(30460122)
【分類號】：R599.1

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