本文選題：糖尿病足 + 熱激蛋白70��； 參考：《昆明醫(yī)科大學(xué)》2017年碩士論文

【摘要】：[目的]通過觀察未合并足部潰瘍的糖尿病患者、合并有足部潰瘍的糖尿病患者以及無糖尿病的正常對照組人群血清中熱激蛋白70(HSP70)水平的變化,以及熱激蛋白70(HSP70)與一些臨床基本資料、生化指標(biāo)的相關(guān)性,從而來初步探討熱激蛋白70(HSP70)在糖尿病及糖尿病足潰瘍(DFU)發(fā)生、發(fā)展過程中的作用,為糖尿病足潰瘍(DFU)早期防治提供一定的理論依據(jù)。[方法]選取2015年9月至2016年9月昆明總醫(yī)院收治的2型糖尿病(T2DM)患者,將未合并足部潰瘍的糖尿病患者30例(DM組)、合并有足部潰瘍的糖尿病患者30例(DFU組)以及無糖尿病的正常對照組人群30例(NC組)分別納入研究。測定所有入選對象空腹靜脈血甘油三酯(TG)、總膽固醇(TC)、高密度脂蛋白膽固醇(HDL-C)、低密度脂蛋白膽固醇(LDL-C)、糖化血紅蛋白(HbA1c%)、空腹血糖(FPG)、餐后2小時(shí)血糖(2hPG)以及血清中熱激蛋白70(HSP70)的水平,同時(shí)通過10g尼龍單絲試驗(yàn)、下肢血管超聲檢查對DM組和DFU組的周圍神經(jīng)病變及下肢血管病變情況進(jìn)行評估,記錄相關(guān)數(shù)據(jù)后最后進(jìn)行對比分析。所有資料采用SPSS17.0軟件,兩組間計(jì)量資料比較采用t檢驗(yàn),計(jì)數(shù)資料比較采用x2檢驗(yàn),相關(guān)性分析采用Pearson、Spearman及多重線性回歸相關(guān)分析法,P0.05為有統(tǒng)計(jì)學(xué)意義。[結(jié)果]①DM組的空腹靜脈血中HbA1c、FPG、2hPG、HSP70水平明顯高于NC組,存在顯著差異(P0.01)。②DFU組的空腹靜脈血中LDL-C、HbAlc%、FPG、2hPG、HSP70水平以及10g尼龍單絲檢測、下肢血管超聲檢查結(jié)果的異常率均明顯高于DM組,差異有統(tǒng)計(jì)學(xué)意義(P0.05);③DM組和DFU組患者的體重指數(shù)(BMI)、血LDL-C水平、10g尼龍單絲檢測及下肢血管超聲檢查結(jié)果異常率與血清HSP70水平之間存在顯著相關(guān)性(P0.05)。[結(jié)論]①血清熱激蛋白70可能參與了糖尿病及糖尿病足部潰瘍發(fā)生、發(fā)展的病理生理過程。②合理調(diào)節(jié)低密度脂蛋白膽固醇水平、控制體重,以及加強(qiáng)對糖尿病患者神經(jīng)病變、血管病變的篩查可能一定程度上能預(yù)防和延緩糖尿病足部潰瘍病變的發(fā)生發(fā)展。
[Abstract]:[objective] to observe the changes of serum heat shock protein 70 (HSP70) levels in diabetic patients without foot ulcers, diabetic patients with foot ulcers and normal controls without diabetes mellitus.The relationship between heat shock protein 70 (HSP70) and some basic clinical data and biochemical indexes was also discussed to explore the role of heat shock protein 70 (HSP70) in the pathogenesis and development of diabetes mellitus and diabetic foot ulcer.To provide a theoretical basis for early prevention and treatment of diabetic foot ulcer.[methods] Type 2 diabetes mellitus (T2DM) patients treated in Kunming General Hospital from September 2015 to September 2016 were selected.30 diabetic patients without foot ulcers, 30 diabetic patients with foot ulcers, 30 diabetic patients with foot ulcers (DFU group) and 30 normal controls without diabetes mellitus (NC group) were included in the study.The levels of triglyceride triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), glycosylated hemoglobin (HbA1cT), fasting blood glucose (FPGG), 2 hours postprandial blood glucose (2 hPGs) and serum heat shock protein (70 HSP70) were measured in all subjects.At the same time, 10 g nylon monofilament test and lower extremity vascular ultrasound were used to evaluate peripheral neuropathy and lower extremity vascular lesion in DM group and DFU group.All the data were analyzed by SPSS17.0 software. T test was used to compare the measurement data between the two groups and x2 test was used to compare the counting data. The correlation analysis was conducted by Pearsonian Spearman and multiple linear regression correlation analysis (P0.05).[results] the level of HSP70 in fasting venous blood of 1DM group was significantly higher than that of NC group. There was a significant difference in the level of HSP70 and 10 g nylon monofilament in fasting venous blood of 1DM group. The abnormal rate of lower extremity vascular ultrasound was significantly higher than that of DM group.There was a significant difference in body mass index (BMI) between P0.05DM-group and DFU group. There was a significant correlation between the abnormal rate of 10 g nylon monofilament in serum LDL-C level and the serum HSP70 level.[conclusion] 1Serum heat shock protein 70 may be involved in the development of diabetes mellitus and diabetic foot ulcers.Strengthening the screening of diabetic neuropathy and vascular disease may prevent and delay the occurrence and development of diabetic foot ulcerative disease to some extent.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R587.2

【參考文獻(xiàn)】

中國期刊全文數(shù)據(jù)庫 前10條

1 李崢t，

本文編號：1754084