本文選題：乳鐵蛋白 + 骨質(zhì)疏松��； 參考：《福建醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的:本研究旨在觀察乳鐵蛋白(Lactoferrin LF)對(duì)去卵巢大鼠IGF-IR/IGFBP-2/IGFBP-4m RNA基因表達(dá)的影響,探討乳鐵蛋白是否通過(guò)介導(dǎo)IGF-IR/IGFBP-2/IGFBP-4m RNA基因表達(dá)機(jī)制治療絕經(jīng)后骨質(zhì)疏松。方法:選取6個(gè)月大小,體重約在270g左右,雌性SD(Sprague-Dawley)大鼠70只,適應(yīng)性喂養(yǎng)1周后,入組大鼠隨機(jī)分為去卵巢模型組(Ovx)60只與假手術(shù)組(Sham)10只,把去卵巢大鼠模型組再次分組,隨機(jī)分為不同干預(yù)治療組6組,骨質(zhì)疏松模型組(Con組)10只,實(shí)驗(yàn)中作為對(duì)照組:[蒸餾水2ml/(只·天)灌胃],牛血清白蛋白組(BSA組)10只:[100mg/kg/d+蒸餾水2ml,灌胃],不同濃度劑量的乳鐵蛋白治療組LF1-3(LF1、LF2和LF3組各10只):[0.1g/(kg·d)+蒸餾水2ml,1g/(kg·d)+蒸餾水2ml,2g/(kg·d)+蒸餾水2ml,灌胃],雌激素治療組(E2組)10只[0.1 mg/(kg·周),肌肉注射]。連續(xù)干預(yù)治療6個(gè)月后,通過(guò)觀察乳鐵蛋白干預(yù)前后,體重有無(wú)明顯差異;通過(guò)制作HE石蠟病理切片,使用顯微鏡觀察骨組織結(jié)構(gòu)變化。再通過(guò)Real Time RT-PCR法即實(shí)時(shí)熒光定量聚合酶鏈反應(yīng),檢測(cè)去卵巢大鼠骨組織IGF-1R、IGFBP-2、IGFBP-4m RNA表達(dá)的改變。結(jié)果:1.乳鐵蛋白干預(yù)前,各組大鼠體重均無(wú)明顯差異,均P0.05,大鼠去卵巢手術(shù)后,以各種方法干預(yù)治療6個(gè)月后,除了E2組外,其余各組大鼠體重均明顯升高,均P0.05,提示差別有統(tǒng)計(jì)學(xué)意義,體重增加最多的組為Con組,Con組大鼠均明顯重于Sham組和E2組,均P0.05,提示差別有統(tǒng)計(jì)學(xué)意義,以不同濃度乳鐵蛋白進(jìn)行干預(yù)的LF1-3組及牛血清白蛋白組大鼠體重與Con組相比,雖然均比Con組體重低,但均P0.05,提示無(wú)統(tǒng)計(jì)學(xué)意義。2.骨組織HE染色病理切片顯示:Sham組、E2組骨小梁排列密集、飽滿,間距小,細(xì)胞數(shù)量多;而Con組、BSA組骨小梁變細(xì)、斷裂,骨小梁距離增寬,排列稀疏,細(xì)胞數(shù)量少,骨小梁結(jié)構(gòu)出現(xiàn)較大的空白區(qū)域;而乳鐵蛋白灌胃組LF1-3介于二者之間,且骨小梁排列緊密程度LF3好于LF2,LF2好于LF1。表明成功建立去卵巢大鼠骨質(zhì)疏松模型。3.實(shí)時(shí)熒光定量PCR結(jié)果顯示:Con組IGFBP-2、IGFBP-4 m RNA相對(duì)于Sham組,相對(duì)表達(dá)量均顯著增高,均P0.01,差別有顯著統(tǒng)計(jì)學(xué)意義;相對(duì)于Sham組,Con組IGF-1Rm RNA相對(duì)表達(dá)量明顯降低,均P0.01,差別有顯著統(tǒng)計(jì)學(xué)意義;與Con組比較LF1-3組(100 mg/kg,1 g/kg and 2 g/kg)與E2組IGFBP-2、IGFBP-4m RNA相對(duì)表達(dá)量明顯下降,且隨著乳鐵蛋白濃度的升高下降得越明顯,均P0.01,差別有顯著統(tǒng)計(jì)學(xué)意義;而LF2-3組(1 g/kg and 2 g/kg)與E2組IGF-1R m RNA相對(duì)表達(dá)量均顯著高于Con組,且隨著乳鐵蛋白濃度的升高上升得越明顯,均P0.05,差別有統(tǒng)計(jì)學(xué)意義,牛血清白蛋白BSA組(100mg/kg/d)IGF-1R、IGFBP-2、IGFBP-4m RNA相對(duì)表達(dá)量相對(duì)于Con組基本不變,均P0.05,兩者之間無(wú)統(tǒng)計(jì)學(xué)意義。結(jié)論:1.成功建立去卵巢大鼠骨質(zhì)疏松模型。2.乳鐵蛋白可能通過(guò)抑制IGFBP-2、IGFBP-4 m RNA水平,促進(jìn)IGF-1R m RNA水平的表達(dá)來(lái)改善骨質(zhì)疏松。
[Abstract]:Objective: the purpose of this study was to observe the effects of lactoferrin (Lactoferrin LF) to IGF-IR/IGFBP-2/IGFBP-4m RNA gene expression in ovariectomized rats, to investigate whether lactoferrin mediated by IGF-IR/IGFBP-2/IGFBP-4m RNA gene expression mechanism for treatment of postmenopausal osteoporosis. Methods: a total of 6 months, weight about 270g, female SD (Sprague-Dawley) 70 rats. After 1 weeks of feeding, the rats were randomly divided into ovariectomized group (Ovx) 60 rats with sham operation group (Sham) 10, the ovariectomized rat model group again group, were randomly divided into intervention group with 6 group, osteoporosis model group (Con group) 10. In the experiment as the control group: distilled water 2ml/ (only day) by gavage, bovine serum albumin group (group BSA) 10: [100mg/kg/d+ 2ml of distilled water by gavage, different doses of lactoferrin in treatment group LF1-3 (LF1, LF2 and LF3 group with 10 rats in each group: [0.1g/ (kg - D)) + steamed The distilled water 2ml, 1g/ (kg - D) + distilled water 2ml, 2g/ (kg - D) + 2ml of distilled water by gavage, estrogen treatment group (group E2) 10 [0.1 mg/ (kg), intramuscular injection of continuous intervention. After 6 months of treatment, observed before and after intervention by lactoferrin. There is no obvious difference in weight; through the production of HE paraffin sections, using microscope to observe the microstructure changes of bone. Then Real Time RT-PCR real-time fluorescence quantitative polymerase chain reaction, detection of bone tissue of ovariectomized rats IGF-1R, IGFBP-2, IGFBP-4m RNA expression changes. Results: 1. lactoferrin before intervention showed no significant difference., the body weight of the rats were P0.05 in ovariectomized rats after surgery, with various methods of intervention after 6 months of treatment, except for E2 group, body weight of the rats were significantly increased, P0.05, suggesting that there was a statistically significant difference, the largest increase in weight of group Con, Con group rats were significantly heavier in the Sham group and E2 group, P0.05, suggesting that there was a statistically significant difference, LF1-3 group and bovine serum albumin in rats compared with Con group with different concentrations of lactoferrin intervention, although low body weight was higher than Con group, but P0.05, showed no statistically significant.2. HE staining of bone tissue pathology showed: Sham group, E2 group of trabecular bone densely arranged, plump, small spacing, number of cells; and Con group, BSA group, trabecular bone became thin, fracture, bone trabecula widened the distance, sparse, small numbers of cells, bone trabecula appeared large blank area; between lactoferrin gavage group LF1-3 between the two, and the small bone the beam of closely spaced degree of LF3 is better than LF2, LF2 in LF1. showed that the successful establishment of osteoporosis in ovariectomized rats model of.3. real-time fluorescence quantitative PCR showed that: Con group IGFBP-2, IGFBP-4 m RNA compared with Sham group, the relative expression of P0.01 were significantly increased, there is a significant difference, statistically significant Yi; compared with Sham group, Con group, IGF-1Rm RNA expression was obviously decreased, P0.01, the difference was significant; compared with group Con and group LF1-3 (100 mg/kg, 1 g/kg and 2 g/kg) and IGFBP-2 E2 group, IGFBP-4m RNA expression was obviously decreased, and increased with the increasing concentration of lactoferrin decreased more obviously that was P0.01, the difference was significant; and group LF2-3 (1 g/kg and 2 g/kg) and the relative expression of E2 IGF-1R m in RNA group were significantly higher than Con group, and with the increase of lactoferrin concentration increased more obviously, P0.05, the difference was statistically significant, bovine serum albumin group (100mg/kg/d BSA) IGF-1R, IGFBP-2, the relative expression compared with Con group P0.05 IGFBP-4m RNA were basically unchanged, and no statistical significance between the two. Conclusion: 1. successfully established osteoporosis in ovariectomized rats model of.2. lactoferrin possibly through inhibition of IGFBP-2, IGFBP-4 m RNA, pro The expression of IGF-1R m RNA levels to improve osteoporosis.

【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R580

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 杜丹丹;藍(lán)旭華;薛英;侯建明;;乳鐵蛋白對(duì)去卵巢大鼠骨代謝的影響[J];福建醫(yī)藥雜志;2011年02期

2 肖暉;劉寧;;乳鐵蛋白對(duì)大鼠成骨細(xì)胞增殖與分化的影響[J];國(guó)際骨科學(xué)雜志;2008年03期

3 薛英;侯建明;林慶明;藍(lán)旭華;吳暉南;;乳鐵蛋白對(duì)去卵巢大鼠骨密度及骨M-CSF,IL-6mRNA的影響[J];中華骨質(zhì)疏松和骨礦鹽疾病雜志;2012年02期

4 王復(fù)興,王熙春;密骨疏治療骨質(zhì)疏松癥病例報(bào)告[J];中國(guó)骨質(zhì)疏松雜志;2003年01期

5 馬艷芬;李萬(wàn)根;陳澍;;胰島素和IGF-1對(duì)成骨樣細(xì)胞MG63 Ⅰ型膠原基因表達(dá)的影響[J];中國(guó)骨質(zhì)疏松雜志;2006年05期

6 高威;張巧;;乳鐵蛋白對(duì)去卵巢大鼠骨密度和骨形態(tài)學(xué)的影響[J];中國(guó)骨質(zhì)疏松雜志;2009年09期

，

本文編號(hào)：1746070