本文選題：型糖尿病 + 腸道菌群��； 參考：《山東醫(yī)藥》2017年16期

【摘要】：目的探討2型糖尿病(T2DM)患者腸道菌群變化的臨床意義。方法選取8例T2DM患者與10例體檢健康者,提取其糞便基因組DNA,應(yīng)用菌屬16S rRNA V4序列特異性引物,采用高通量測序技術(shù)分析腸道菌群多樣性(以香農(nóng)多樣性指數(shù)表示)及種類(以豐度表示)。觀察不同性別T2DM患者的腸道菌群種類差異,分析T2DM患者腸道優(yōu)勢菌屬與空腹血糖(FPG)、餐后1 h血糖(1 h PG)和餐后2 h血糖(2 h PG)的關(guān)系。結(jié)果 T2DM患者與體檢健康者香農(nóng)多樣性指數(shù)分別為3.70±0.29、3.30±0.20,二者比較P0.05。門水平下T2DM患者和體檢健康者的腸道共同優(yōu)勢菌門為厚壁菌門、擬桿菌門、變形菌門和放線菌門,二者上述4種優(yōu)勢菌門豐度比較差異均無統(tǒng)計(jì)學(xué)意義(P均0.05)。屬水平下T2DM患者和體檢健康者的腸道共同優(yōu)勢菌屬為擬桿菌屬和毛螺桿菌科,體檢健康者羅斯氏菌屬豐度高于T2DM患者(P0.05)。女性T2DM患者擬桿菌屬科豐度高于男性患者,假單胞菌屬豐度低于男性患者(P均0.05)。T2DM患者柔嫩梭菌屬豐度與2 h PG呈正相關(guān)(r=0.75,P0.05),其他腸道優(yōu)勢菌屬與FPG、1 h PG、2 h PG均無關(guān)(P均0.05)。結(jié)論 T2DM患者腸道內(nèi)存在菌群失調(diào),并具有性別差異;柔嫩梭菌屬與血糖水平有關(guān),可為T2DM的治療提供一種新的思路。
[Abstract]:Objective to investigate the clinical significance of intestinal flora changes in patients with type 2 diabetes mellitus (T 2 DM).Methods the fecal genomic DNA was extracted from 8 T2DM patients and 10 healthy controls. The 16s rRNA V4 sequence specific primers were used.The diversity of intestinal flora (expressed by Shannon's diversity index) and species (denoted by abundance) were analyzed by high-throughput sequencing technique.To observe the differences of intestinal flora in patients with T2DM of different genders, and to analyze the relationship between the predominant bacteria in the intestine of T2DM patients and fasting blood glucose (FPG), postprandial blood glucose (1 h) and postprandial blood glucose (2 h).Results the Shannon diversity index was 3.70 鹵0.293.30 鹵0.20 in T2DM patients and healthy controls, respectively (P 0.05).The common dominant bacteria phylum of intestinal tract in T2DM patients and healthy persons were phylum thunbergium, Bacteroides, Proteus and Actinomycetes. There was no significant difference in the abundance of the above four dominant bacteria between them (P < 0.05).The common intestinal dominant bacteria in T2DM patients and healthy individuals were Bacteroides and Helicobacteriaceae. The abundance of Roxella in healthy individuals was higher than that in T2DM patients (P 0.05).The abundance of Pseudomonas in female patients was higher than that in male patients, and the abundance of Clostridium tenella in patients with 0.05).T2DM was significantly lower than that in patients with 0.05).T2DM. There was a positive correlation between the abundance of Clostridium tenella and 2 h PG.Conclusion there is a maladjustment of intestinal flora in patients with T2DM, and there is a sex difference between them, and Clostridium tenella is related to blood glucose level, which may provide a new idea for the treatment of T2DM.
【作者單位】： 江蘇大學(xué)附屬醫(yī)院;天益健康科學(xué)研究院;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(81570721;81370965) 江蘇省自然科學(xué)基金資助項(xiàng)目(BK20151331) “六大人才高峰”第十二批高層次人才項(xiàng)目(2015-WSN-006) 鎮(zhèn)江市科技支撐-社會發(fā)展重點(diǎn)項(xiàng)目(SH2015028)
【分類號】：R587.1
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本文編號：1733990