本文選題：艾塞那肽　切入點：心肌細胞　出處：《山東醫(yī)藥》2017年06期

【摘要】：目的觀察GLP-1類似物艾塞那肽(EX-4)對高糖所致新生乳鼠心肌細胞鈣信號紊亂的調(diào)控作用,探討GLP-1的心臟保護機制。方法分離新生乳鼠心肌細胞,培養(yǎng)24 h后隨機分為對照組、高糖組和高糖+EX-4組,分別加入25 mmol/L甘露醇、25 mmol/L葡萄糖、25 mmol/L葡萄糖+10 nmol/L EX-4,繼續(xù)培養(yǎng)24 h。使用激光共聚焦顯微技術(shù)檢測各組心肌細胞自發(fā)鈣火花發(fā)放頻率、場刺激鈣瞬變幅度、鈣庫容量;使用膜片鉗技術(shù)記錄L型鈣通道電流。結(jié)果對照組、高糖組、高糖+EX-4組的心肌細胞自發(fā)鈣火花發(fā)放頻率分別為(1.35±0.07)、(3.86±0.27)、(1.99±0.15)個/(μm·s),場刺激鈣瞬變幅度鈣瞬變幅度分別為3.95±0.08、3.34±0.07、4.09±0.12,鈣庫容量分別為4.95±0.18、3.26±0.09、4.59±0.15,10 m V時的L型鈣通道電流密度分別為-12.46±0.58、-11.28±0.55、-13.09±0.64,高糖組心肌細胞鈣火花發(fā)放頻率、鈣瞬變幅度較對照組升高,鈣庫容量、電流密度較對照組降低(P0.05或0.01),高糖+EX-4組鈣火花發(fā)放頻率、鈣瞬變幅度較高糖組降低,鈣庫容量、電流密度較高糖組升高(P0.05或0.01),與對照組比較無統(tǒng)計學意義。結(jié)論 EX-4能夠增強心肌細胞L型鈣通道電流,減少肌漿網(wǎng)鈣漏流,從而有效糾正高糖所致心肌細胞鈣調(diào)控紊亂,降低高糖所致心肌損傷。
[Abstract]:Aim to investigate the effects of EX-4, a GLP-1 analogue, on calcium signal disturbance in neonatal neonatal rat cardiomyocytes induced by high glucose, and to explore the cardioprotective mechanism of GLP-1.Methods Neonatal neonatal rat cardiomyocytes were isolated and cultured for 24 h and were randomly divided into three groups: control group, high glucose group and high glucose EX-4 group. 25 mmol/L mannitol 25 mmol/L glucose 25 mmol/L glucose 10 nmol/L EX-4 were added respectively, and then cultured for 24 h.Laser confocal microscopy was used to detect the spontaneous calcium discharge frequency of cardiac myocytes, the amplitude of calcium transient and the capacity of calcium reservoir, and the patch-clamp technique was used to record the L-type calcium channel current.Results the control group, high sugar group,Frequency of release of calcium sparks in cardiomyocytes,The calcium transient amplitude was higher than that of the control group, the calcium storage capacity and current density were lower than those of the control group (P0.05 or 0.01), the calcium sparks release frequency and the calcium transient amplitude of the high sugar EX-4 group were lower than those of the high sugar group, and the calcium storage capacity was lower than that of the high sugar group.The current density was higher than that of high glucose group (P0.05 or 0.01), and had no statistical significance compared with the control group.Conclusion EX-4 can enhance L-type calcium channel current and reduce calcium leakage in sarcoplasmic reticulum, which can effectively correct calcium regulation disorder and reduce myocardial injury induced by high glucose.
【作者單位】： 深圳市第二人民醫(yī)院;深圳大學醫(yī)學院;
【基金】：國家自然科學基金資助項目(31400982) 深圳市科技計劃項目(JCYJ20140416180300394,JCYJ20150330102720148,JCYJ20140418182819136)
【分類號】：R587.2;R54

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