發(fā)布時(shí)間：2018-03-27 21:04

  本文選題：艾滋病病毒　切入點(diǎn)：限制性抗原親和力法　出處：《現(xiàn)代預(yù)防醫(yī)學(xué)》2016年01期

【摘要】：目的評(píng)價(jià)LAg-Avidity EIA法用于HIV-1新發(fā)感染檢測(cè)的性能。方法應(yīng)用LAg-Avidity EIA法檢測(cè)617份HIV-1陽性樣本(包括123份接受抗病毒治療的艾滋病晚期病人樣本和494份2014年江西省新報(bào)告HIV-1感染者樣本),比較試劑盒中陰性質(zhì)控品(NC)、校準(zhǔn)品(CAL)、弱陽性質(zhì)控品(LPC)和強(qiáng)陽性質(zhì)控品(HPC)的OD值和OD(n)值的平均值、標(biāo)準(zhǔn)差和變異系數(shù),比較144份樣本的初篩試驗(yàn)和確認(rèn)試驗(yàn)結(jié)果的一致性,計(jì)算LAg-Avidity EIA法對(duì)于抗病毒治療感染樣本的誤判率。結(jié)果 16塊酶標(biāo)板的陰性質(zhì)控品、校準(zhǔn)品、弱陽性質(zhì)控品和強(qiáng)陽性質(zhì)控品OD值的變異系數(shù)在5%~19%之間,經(jīng)校準(zhǔn)品校準(zhǔn)后陰性質(zhì)控品、弱陽性質(zhì)控品和強(qiáng)陽性質(zhì)控品OD-n值的變異系數(shù)繼續(xù)減小至7%之內(nèi);144份樣本初篩試驗(yàn)和確認(rèn)試驗(yàn)OD-n值相關(guān)系數(shù)R2=0.95;LAg-Avidity EIA法將123份接受抗病毒治療的艾滋病晚期病人樣本中的19份誤判為近期感染,誤判率為15.45%(19/123)。結(jié)論 LAg-Avidity EIA法檢測(cè)穩(wěn)定性和重復(fù)性均較好,可以廣泛用于HIV-1新發(fā)感染檢測(cè),但是進(jìn)行新發(fā)感染檢測(cè)前需剔除抗病毒治療樣本。
[Abstract]:Objective to evaluate the performance of LAg-Avidity EIA method in the detection of new HIV-1 infections. Methods 617 HIV-1 positive samples (including 123 Aids patients receiving antiviral therapy and 494 new HIV-1 reports in Jiangxi Province in 2014) were detected by LAg-Avidity EIA method. The mean values of OD and ODN values of negative quality control products, calibration products, weak positive quality control products and strong positive quality control products were compared. Standard deviation and coefficient of variation were compared. The results of screening test and confirmation test were compared, and the misjudgment rate of LAg-Avidity EIA method for antiviral treatment of infected samples was calculated. The coefficient of variation of OD value of weak positive quality control and strong positive quality control is between 5% and 19%. After calibrating, the negative quality control is negative. The coefficient of variation of OD-n value of weakly positive quality control and strong positive quality control continued to decrease to within 7% of 144 samples. The correlation coefficient of OD-n value between primary screening test and confirmation test was R20.95% LAg-Avidity EIA method. 123 samples of advanced AIDS patients receiving anti-viral therapy were collected by the method of LAg-Avidity EIA. Nineteen of them were misjudged to be recent infections. Conclusion LAg-Avidity EIA method has good stability and reproducibility, and can be widely used in the detection of new infection of HIV-1, but antiviral treatment samples should be removed before the detection of new infection.
【作者單位】： 江西省疾病預(yù)防控制中心;
【分類號(hào)】：R512.91

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