本文選題：2型糖尿病　切入點(diǎn)：西格列汀　出處：《安徽醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的研究西格列汀對(duì)2型糖尿病(T2DM)大鼠各代謝指標(biāo)及脂肪因子chemerin水平的影響及其可能的機(jī)制,并探討脂肪因子chemerin與T2DM的關(guān)系。方法1.選取年齡4周且體重為(100±20)g的普通級(jí)雄性SD大鼠,共35只,在室溫為22℃及濕度在55%左右的環(huán)境下不限制攝食及飲水喂養(yǎng)。2.造模:將大鼠分為普通飼料喂養(yǎng)組(NC組,n=10)及高脂飼料喂養(yǎng)組(HF組,n=25),12周后一次性腹腔注射鏈脲佐菌素STZ(用之前溶解在PH=4.5的0.1mmol/L檸檬酸緩沖液中)35 mg/kg于高脂大鼠,7天后測(cè)定大鼠空腹血糖,將空腹血糖11.1 mmol/L設(shè)定為臨界點(diǎn),超過該臨界點(diǎn)的大鼠認(rèn)為T2DM模型造模成功。3.干預(yù)方式:將20只造模成功的大鼠隨機(jī)分為2組:T2DM對(duì)照組(T2DM組,n=10):灌胃2ml生理鹽水,西格列汀組(SITA組,n=10):灌胃給予西格列汀10mg/kg/d,溶于2 ml羥甲基淀粉酶中,每日灌胃時(shí)間于早9點(diǎn),持續(xù)6周,繼續(xù)予以普通飼料喂養(yǎng)。4.留取標(biāo)本:干預(yù)前禁食12 h后內(nèi)眥靜脈取血2 ml,干預(yù)6周后禁食12 h腹主動(dòng)脈取血3 ml,血標(biāo)本靜止15 min后,3 200 r/min離心10 min,留取血清置于-80℃冰箱保存。并在冰上操作取出腎周脂肪、肝臟組織及股側(cè)肌肉組織,置于液氮罐中以備提取蛋白質(zhì)。5.檢測(cè)指標(biāo)及方法:干預(yù)前后測(cè)體重(BW),ELISA法測(cè)定血清chemerin、胰島素(FINS),血糖儀測(cè)定空腹血糖(FBG),酶法及選擇性沉淀法測(cè)定血脂包括總膽固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL),通過公式計(jì)算胰島素抵抗指數(shù)(HOMO-IR)與胰島β細(xì)胞功能指數(shù)(HOMO-β),Western blot法檢測(cè)大鼠腎周脂肪、肝臟和肌肉組織chemerin蛋白表達(dá)水平。結(jié)果1.西格列汀干預(yù)前:T2DM大鼠的血清chemerin、BW、FBG、FINS、TC、TG、LDL、HOMO-IR與NC組比較均增高,HOMO-β降低,T2DM組大鼠腎周脂肪、肝臟及肌肉組織的chemerin蛋白表達(dá)量增加(P0.05)2.西格列汀干預(yù)后,與T2DM組比較,SITA組BW、FBG、FINS、TC、TG、LDL、HOMO-IR及血清chemerin水平降低,HOMO-β升高(P0.05)。SITA組大鼠腎周脂肪、肝臟組織的chemerin蛋白表達(dá)量減少(P0.05),肌肉組織中chemerin表達(dá)量無變化。3.相關(guān)性分析:西格列汀干預(yù)前血清chemerin與BW、FBG、FINS、HOMO-IR、TG、TC及LDL呈正相關(guān),干預(yù)后血清chemerin與BW、FINS、TG及HOMO-IR呈正相關(guān),血清中chemerin的含量與腎周脂肪中該蛋白的表達(dá)量呈正相關(guān)(r=0.79,P0.05)。4.多元回歸分析:顯示西格列汀干預(yù)前后TG、FINS及HOMO-IR始終與chemerin獨(dú)立相關(guān)。血清chemerin水平與腎周脂肪chemerin蛋白表達(dá)量呈正相關(guān)(r=0.79,P0.05)。結(jié)論2型糖尿病大鼠血清及脂肪、肝臟、肌肉組織中chemerin水平較非糖尿病大鼠增加,提示在多種代謝性疾病如肥胖、2型糖尿病的發(fā)病過程中,脂肪因子chemerin的可能有重要的作用。西格列汀可降低血清、腎周脂肪及肝臟組織中chemerin水平,改善血清學(xué)各代謝指標(biāo)。
[Abstract]:Objective to study the Sig Leo Dean of type 2 diabetes mellitus (T2DM) rats of various metabolic indicators and fat levels of factor Chemerin and its possible mechanism, and to explore the relationship of adipokine Chemerin and T2DM. Methods 1. aged 4 weeks and weighing (100 + 20) ordinary male SD g rats, a total of 35. At room temperature is 22 degrees and the humidity is about 55% under the environment of restricted feeding and drinking feeding.2. rats: the rats were divided into normal diet group (group NC, n=10) and high-fat diet group (group HF, n=25), 12 weeks after intraperitoneal injection of streptozotocin (STZ before the use of 0.1mmol/L dissolved in citrate buffer in PH=4.5 35 mg/kg) in hyperlipidemia rats, 7 days after the determination of fasting blood glucose, fasting blood glucose of 11.1 mmol/L was set as the critical point, more than the critical point that rat T2DM model was successfully built.3. intervention: 20 successful model of large rats were randomly divided into 2 groups: T2DM control group (group T2DM, n=10) 2ml gavage of saline, Sig Leo Dean group (group SITA, n=10): intragastric administration of Sig Leo Dean 10mg/kg/d, dissolved in 2 ml hydroxymethyl amylase, daily gavage time in early 9, for 6 weeks, continue to be fed with normal diet.4. specimens specimen: before intervention after fasting for 12 h angular vein blood 2 ml, 6 weeks after the intervention 12 h fasting blood from the abdominal aorta 3 ml blood samples were still 15 min after 3200 r/min centrifugation for 10 min, the serum -80 in C refrigerator. In the ice and pulled out the perirenal fat and liver tissue and the side muscle tissue in liquid nitrogen tank for extracting detection index and method of protein.5.: measurement of body weight before and after the intervention (BW), serum Chemerin ELISA, insulin (FINS), fasting blood glucose (FBG), serum lipids including total cholesterol enzymatic and selective precipitation method (TC), triglyceride (TG), low density lipoprotein 鐧，

本文編號(hào)：1661417