發(fā)布時間：2018-03-24 01:34

  本文選題：高脂飲食　切入點：型糖尿病　出處：《四川動物》2016年01期

【摘要】：觀察高脂飲食聯(lián)合鏈脲佐菌素(STZ)對小鼠糖脂代謝及胰島功能損傷的影響。將體質(zhì)量為18~20 g的SPF級雄性C57BL/6J小鼠40只,隨機分為對照組和模型組,每組20只。對照組小鼠飼喂對照飼料,模型組小鼠飼喂高脂飼料。1周后,禁食16 h,測量小鼠體質(zhì)量與空腹血糖;尾靜脈采血,分離血清;每周1次,連續(xù)4周。4周后,對照組小鼠腹腔注射檸檬酸緩沖液,模型組小鼠腹腔注射STZ,劑量為40 mg·kg~(-1),每天1次,連續(xù)3 d。繼續(xù)飼養(yǎng)2周后,測量隨機血糖,以小鼠血糖≥16.7 mmol·L~(-1)者即判定為2型糖尿病。對照組及2型糖尿病小鼠經(jīng)腹腔注射葡萄糖以進行糖耐量試驗。與對照組同一時間的體質(zhì)量和血糖進行比較,模型組小鼠體質(zhì)量、血糖均升高,除第1周血糖外,差別均有統(tǒng)計學(xué)意義(P0.05)。采用重復(fù)測量方差分析,發(fā)現(xiàn)喂養(yǎng)時間對體質(zhì)量(F=200.831)和血糖(F=7.025)均有影響,差異有統(tǒng)計學(xué)意義(P0.05);不同喂養(yǎng)時間對低密度脂蛋白(F=30.793)、甘油三酯(F=34.027)和高密度脂蛋白(F=30.793)均有影響,差異有統(tǒng)計學(xué)意義(P0.05)。不同飼料喂養(yǎng)與不同喂養(yǎng)時間對體質(zhì)量和甘油三酯存在交互作用(P0.05)。比較糖耐量曲線發(fā)現(xiàn),成模小鼠較對照組小鼠糖耐量降低。采用ELISA試劑盒檢測小鼠血清中TNF-α含量,成模小鼠的TNF-α含量高于對照組,差異有統(tǒng)計學(xué)意義(P0.05)。高脂飲食可導(dǎo)致C57BL/6J小鼠糖脂代謝紊亂,給予STZ后引起了小鼠糖耐量降低及炎癥損傷,高脂飲食聯(lián)合STZ可提高小鼠2型糖尿病模型的成模率。
[Abstract]:To observe the effects of high-fat diet combined with streptozotocin (STZ) on glycolipid metabolism and islet function injury in mice, 40 male C57BL/6J mice of SPF grade with body weight of 1820 g were randomly divided into control group and model group. 20 mice in each group were fed with control diet, and mice in model group were fed with high fat diet for 1 week, fasting for 16 hours, body mass and fasting blood glucose were measured, blood was collected from tail vein and serum was separated, once a week, after 4 weeks, Citric acid buffer was injected intraperitoneally in the control group and STZ-1 was injected intraperitoneally in the model group at a dose of 40 mg / kg, once a day for 3 days. Type 2 diabetes was determined by blood glucose 鈮，

本文編號：1656147