本文選題：縫隙連接　切入點：免疫　出處：《石河子大學》2017年碩士論文

【摘要】：目的:通過比較正常Sprague-Dawley(SD)大鼠和圍絕經(jīng)期模型的SD大鼠,正常育齡期女性和圍絕經(jīng)期女性外周血T淋巴細胞亞群、CD4+和CD8+T淋巴細胞上連接蛋白(Connexin,Cx)40和43表達、以及血清炎癥因子IL-1、IL-2和IL-6的表達,探討E_2(雌二醇)是否通過調(diào)節(jié)T淋巴細胞間縫隙連接通訊,抑制圍絕經(jīng)期低雌激素造成的T淋巴細胞亞群紊亂和促炎因子釋放增加的慢性炎癥過程,進而改善由慢性炎癥引起的免疫功能異常。方法:動物血樣實驗:實驗選用4月齡雌性SD大鼠,分為Control組(對照組)、OVX組(圍絕經(jīng)期動物模型組)及OVX+E_2(雌激素替代治療組)。應(yīng)用流式細胞術(shù)檢測大鼠外周血T淋巴細胞亞群的比例以及CD4+和CD8+T淋巴細胞上CX40和CX43的表達頻率;其次使用ELISA技術(shù)檢測各組間雌二醇(estradiol,E_2)的差異以及各組炎癥因子IL-1、IL-2、IL-6的表達。臨床血樣實驗:分為育齡期組和圍絕經(jīng)期組,應(yīng)用流式細胞術(shù)檢測兩組人群外周血T淋巴細胞亞群的比例以及CD4+和CD8+T淋巴細胞上CX40和CX43的表達頻率;同時使用ELISA技術(shù)檢測各組炎癥因子IL-1、IL-2、IL-6的表達。結(jié)果:1.OVX組SD大鼠E_2水平低于Control組(P0.05),OVX+E_2組E_2水平比OVX組上升(P0.05)。2.OVX組CD4+CD25+T淋巴細胞比Control組上升(P0.05),OVX+E_2組比OVX組CD4+CD25+T淋巴細胞下降(P0.05);OVX組CD4+/CD8+比Control組下降(P0.05),OVX+E_2組CD4+/CD8+比OVX組上升(P0.05)。3.OVX組CD8+CX40、CD4+CX43均比Control組明顯上升(P0.01),OVX+E_2組CD8+CX40、CD4+CX43均比Control組下降(P0.01),OVX+E_2組CD8+CX43比Control組及OVX組均下降(P0.01)。4.OVX組大鼠血漿IL-1β、IL-6濃度較Control均升高(P0.01),IL-2較Control組降低(P0.05),OVX+E_2組IL-1β、IL-6濃度較OVX組均降低(P0.01),IL-2較OVX組升高(P0.05)。5.Pearson相關(guān)性分析結(jié)果顯示,大鼠IL-1β、IL-6與CD8+CX40、CD4+CX43和CD8+CX43均為正相關(guān)關(guān)系;IL-2與CD4+CX43為負相關(guān)關(guān)系。6.圍絕經(jīng)期組CD4+/CD8+比育齡組上升(P0.05)2.圍絕經(jīng)組女性外周血中CD4+CX40、CD4+CX43和CD8+CX43比育齡期組均上升(P0.05)。7.圍絕經(jīng)期女性外周血中IL-2及IL-6濃度均下降(P0.05)。結(jié)論:圍絕經(jīng)期是一種慢性低度炎癥,伴隨T淋巴細胞亞群比值異常,促炎因子釋放增加。外源性給予E_2能夠改善上述由圍絕經(jīng)期伴隨的慢性炎癥造成的損害,其可能機制是E_2通過下調(diào)T淋巴細胞上構(gòu)成縫隙連接通道的CX40和CX43的表達,抑制T淋巴細胞間的信息通訊,進而減少促炎因子的釋放。
[Abstract]:Objective: To compare the normal Sprague-Dawley (SD) rats and perimenopausal model of SD rats, normal women of childbearing age and menopausal women of peripheral blood T lymphocyte subsets, CD4+ lymphocytes and CD8+T junction protein (Connexin, Cx) 40 and 43 expression, and serum IL-1, the expression of IL-2 and IL-6 to investigate, E_2 (estradiol) whether by regulating the gap junctional intercellular communication of T lymphocytes, inhibit the disorder of T lymphocyte subsets in perimenopausal caused by low estrogen and proinflammatory cytokine release process of chronic inflammation increase, and improve the immune function caused by chronic inflammatory abnormalities. Methods: animal experiment: blood test selected 4 month old female SD rats were divided into Control group (control group), OVX group (perimenopausal animal model group) and OVX+E_2 (estrogen replacement therapy group). Rats were detected in peripheral blood T lymphocytes subsets by flow cytometry and CD4 CX40 + and CD8+T lymphocytes and the expression of CX43 frequency; secondly using ELISA technique to detect the estradiol (estradiol, E_2) as well as the differences between each group of inflammatory factors IL-1, IL-2, IL-6. The expression of clinical blood experiment: divided into age group and menopausal group, the proportion of peripheral blood T lymphocyte subsets in two group by flow cytometry and the expression frequency of CD4+ and CD8+T lymphocyte CX40 and CX43; at the same time using the detection of IL-1, each group of inflammatory factors ELISA IL-2, the expression of IL-6. Results: the E_2 level of 1.OVX group SD rats were lower than that of Control group (P0.05), the level of E_2 in group OVX+E_2 than in group OVX increased (P0.05) the.2.OVX group of CD4+CD25+T lymphocytes than in group Control increased (P0.05), OVX+E_2 group was lower than that of OVX group (P0.05); CD4+CD25+T lymphocyte in OVX group CD4+/CD8+ decreased than group Control (P0.05), OVX+E_2 group CD4+/CD8+ than in group OVX increased in group.3.OVX (P0.05) CD8+CX40, CD4+C X43 was higher than Control group significantly increased (P0.01), OVX+E_2 group CD8+CX40, CD4+CX43 were lower than group Control (P0.01), OVX+E_2 CD8+CX43 group than Control group and OVX group were significantly decreased (P0.01) in serum in.4.OVX group IL-1 beta, IL-6 concentration was Control increased (P0.01), IL-2 was lower than that of group Control (P0.05 OVX+E_2), group IL-1 beta, IL-6 concentration in OVX group were lower (P0.01), IL-2 increased compared with group OVX (P0.05).5.Pearson correlation analysis showed that rat IL-1 beta, IL-6 and CD8+CX40, CD4+CX43 and CD8+CX43 were positive correlation between IL-2 and CD4+CX43; negative correlation between.6. in perimenopausal group CD4+/CD8+ than childbearing age group increased (P0.05) 2. perimenopausal women group CD4+CX40 in the peripheral blood of CD4+CX43 and CD8+CX43 than the adult group were increased (P0.05.7.) in perimenopausal women and IL-2 IL-6 concentration in peripheral blood were decreased (P0.05). Conclusion: peri menopause is a chronic low-grade inflammation, with T lymphocyte subsets The ratio of abnormal proinflammatory cytokine release increase. Exogenous E_2 can improve the perimenopausal accompanied by chronic inflammatory damage, the possible mechanism is E_2 by inhibiting the expression of CX40 of gap junction channels and CX43 T lymphocytes, T lymphocytes in the suppression of information communication, thereby reducing the release of inflammatory factors.

【學位授予單位】：石河子大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R711.75

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