發(fā)布時間：2018-03-21 07:24

  本文選題：糖尿病足　切入點：動脈粥樣硬化　出處：《中國動脈硬化雜志》2017年04期 　論文類型：期刊論文

【摘要】：目的探討蛋白激酶C(PKC)活性與糖尿病足截肢患者脛前動脈鈣化之間的相關性。方法選取2012年10月至2016年1月在江蘇大學附屬醫(yī)院骨科行糖尿病足大截肢住院治療的患者共60例,根據(jù)CT對脛前動脈鈣化程度的檢測將患者分為輕度鈣化組(n=20)、中度鈣化組(n=20)及重度鈣化組(n=20)。通過患者臨床基線資料的采集以及脛前動脈組織連續(xù)石蠟切片Von Kossa染色觀察鈣化演進,通過PepTag~汶Assay法檢測組織細胞膜PKC活性,通過Western blot檢測對PKCα亞型進行半定量分析。結果糖尿病足截肢患者脛前動脈Von Kossa染色與相應的動脈CT掃描結果一致:脛前動脈斑塊內的鈣鹽沉積呈點灶狀分布,而且隨著脛前動脈內斑塊的不斷擴大乃至管腔閉塞,鈣顆粒的密度也有不同程度的增加,但始終呈點灶狀分布的微鈣化。定量檢測及Pearson相關性分析顯示PKC活性與糖尿病足脛前動脈鈣化積分正相關(r=0.931,P0.001),進一步分析顯示可能是PKCα亞型發(fā)揮了促進鈣化演進的作用。結論 PKCα亞型的活化可能與糖尿病動脈斑塊內鈣化的演進存在正相關,其水平可能對針對性干預血管鈣化有著一定的價值。
[Abstract]:Objective to investigate the correlation between protein kinase C kinase (PKC) activity and calcification of anterior tibial artery in diabetic foot amputation patients. Methods 60 patients with diabetic foot amputation were treated with diabetic foot amputation from October 2012 to January 2016 in Department of Orthopaedics, affiliated Hospital of Jiangsu University. The patients were divided into mild calcified group, moderate calcified group and severe calcified group according to CT examination of the calcification degree of anterior tibial artery. The clinical baseline data were collected and the Von Kossa staining of the tibial anterior artery tissue was used to observe the progress of calcification. The PKC activity of tissue membrane was measured by PepTag-Wen Assay assay. Results the results of Von Kossa staining of anterior tibial artery in diabetic foot amputation were consistent with those of CT scan. The calcium deposition in the plaque of anterior tibial artery was focal distribution. Moreover, with the enlargement of plaque in anterior tibial artery and the occlusion of lumen, the density of calcium granules increased in varying degrees. Quantitative analysis and Pearson correlation analysis showed that PKC activity was positively correlated with calcification integral of anterior tibial artery of diabetic foot. Further analysis showed that PKC 偽 subtype played a role in promoting calcification progression. Conclusion the activation of PKC 偽 subtype may be positively related to the progression of calcification in diabetic artery plaque. Its level may have certain value for intervention of vascular calcification.
【作者單位】： 江蘇大學附屬醫(yī)院病理科;江蘇大學附屬醫(yī)院心內科;
【基金】：國家自然科學基金項目(81370408、81370409) 江蘇省自然科學基金項目(BK20131246) 江蘇省衛(wèi)生廳項目(Q201308) 鎮(zhèn)江市社會發(fā)展項目(SH2015038、SH2015023、SHW2015001) 衛(wèi)生部核醫(yī)學重點實驗室 江蘇省分子核醫(yī)學重點實驗室開放課題(KF201504) 江蘇大學臨床專項項目(JDLCZX001)
【分類號】：R587.2

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