本文選題：滑膜成纖維細(xì)胞　切入點(diǎn)：膠原誘導(dǎo)的關(guān)節(jié)炎大鼠模型　出處：《南京師大學(xué)報(bào)(自然科學(xué)版)》2017年03期 　論文類型：期刊論文

【摘要】：類風(fēng)濕關(guān)節(jié)炎(Rheumatoid arthritis,RA)病人滑膜成纖維細(xì)胞(Fibroblast-like synoviocytes,FLS)是研究RA發(fā)病機(jī)制和進(jìn)行RA藥物篩選的最理想的細(xì)胞來源.但是對(duì)于眾多非醫(yī)療機(jī)構(gòu)的高校和科研院所而言,由于RA病人滑膜組織不易獲取而難以進(jìn)行細(xì)胞水平的操作.因此,建立膠原誘導(dǎo)的關(guān)節(jié)炎大鼠(Collagen-induced arthritis,CIA)模型,成功分離膝關(guān)節(jié)滑膜組織并進(jìn)行滑膜成纖維細(xì)胞的原代培養(yǎng)和純化,將有效解決RA FLS的來源問題,為后續(xù)實(shí)驗(yàn)奠定基礎(chǔ).本實(shí)驗(yàn)通過皮內(nèi)和皮下兩次注射雞Ⅱ型膠原誘導(dǎo)Wistar大鼠罹患關(guān)節(jié)炎,通過關(guān)節(jié)炎指數(shù)評(píng)分和膝關(guān)節(jié)HE染色明確CIA模型的建立;分離膝關(guān)節(jié)滑膜組織,采用Ⅱ型膠原酶和無EDTA胰酶消化法分離細(xì)胞并進(jìn)行傳代培養(yǎng),傳至第三代時(shí),細(xì)胞免疫熒光檢測(cè)細(xì)胞中VCAM-1蛋白含量以鑒定細(xì)胞純度.結(jié)果顯示,滑膜組織經(jīng)過消化和培養(yǎng)后得到大量大鼠滑膜成纖維細(xì)胞;在顯微鏡下觀察所得細(xì)胞呈梭形樹突狀,具有成纖維樣細(xì)胞特征;VCAM-1蛋白陽性細(xì)胞達(dá)98%以上,確證所分離細(xì)胞為滑膜成纖維樣細(xì)胞,且純度極高.本研究建立了較簡(jiǎn)便快捷地獲得大量滑膜成纖維細(xì)胞的方法,為RA的發(fā)病機(jī)制研究和藥物防治提供了豐富的實(shí)驗(yàn)材料.
[Abstract]:Fibroblast-like synoviocytes-FLSs (Fibroblast-like synoviocytes-FLSs) from patients with rheumatoid arthritis (Rheumatoid arthritis RAA) are ideal cell sources for studying the pathogenesis of RA and screening RA drugs. But for many non-medical institutions and research institutes, Because synovial tissue of RA patients is difficult to obtain, it is difficult to operate at cell level. Therefore, Collagen-induced arthritis CIAA model of collagen-induced arthritis rat was established, and synovial tissue of knee joint was isolated successfully and primary culture and purification of synovial fibroblasts were carried out. It will effectively solve the problem of the origin of RA FLS and lay a foundation for further experiments. In this experiment, Wistar rats were induced to develop arthritis by intradermal and subcutaneous injection of chicken type 鈪，

本文編號(hào)：1637834