本文選題：類風(fēng)濕關(guān)節(jié)炎　切入點(diǎn)：血小板微粒　出處：《四川大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年03期 　論文類型：期刊論文

【摘要】：目的觀察血小板微粒(platelets microparticles,PMPs)在類風(fēng)濕關(guān)節(jié)炎(RA)患者關(guān)節(jié)液和外周血中的表達(dá)及其與健康人外周血對(duì)照之間的差異,探討PMPs水平與RA活動(dòng)指標(biāo)之間的關(guān)系。方法采用流式細(xì)胞術(shù)檢測(cè)26例活動(dòng)期[疾病活動(dòng)指數(shù)(DAS)28≥3.2]RA患者外周血和15例健康對(duì)照組外周血PMPs含量、PMPs的CD62P、CD154陽性比率,檢測(cè)RA患者和15例健康對(duì)照組外周血C反應(yīng)蛋白(CRP)、血沉(ESR)、類風(fēng)濕因子(RF)、抗環(huán)瓜氨酸肽抗體(ACPA)指標(biāo),分析PMPs含量與各指標(biāo)相關(guān)性。其中16例有膝關(guān)節(jié)積液RA患者同時(shí)檢測(cè)配對(duì)外周血和關(guān)節(jié)液PMPs含量及CD62P~+PMPs和CD154~+PMPs比率。結(jié)果 (1)RA患者外周血PMPs含量高于健康對(duì)照組(P0.01),RA患者外周血PMPs含量高于配對(duì)關(guān)節(jié)液(P0.01);(2)RA患者外周血CD62P~+PMPs比率高于健康對(duì)照組(P0.05)和配對(duì)關(guān)節(jié)液(P0.05);(3)RA患者外周血CD154~+PMPs比率高于健康對(duì)照組(P0.01)和配對(duì)關(guān)節(jié)液(P0.05);(4)RA患者外周血PMPs含量與疾病活動(dòng)性評(píng)分DAS28(r=0.462,P=0.018)呈正相關(guān),與ESR、CRP、RF、ACPA不存在相關(guān)性;而關(guān)節(jié)液PMPs含量與CRP、ESR、RF、ACPA、DAS28均無相關(guān)性。結(jié)論 RA患者外周血PMPs表達(dá)異常,且與疾病活動(dòng)性有關(guān),提示PMPs在RA疾病活動(dòng)和關(guān)節(jié)損害中發(fā)揮重要作用。
[Abstract]:Objective to observe the expression of platelets microplatelets PMPsin the synovial fluid and peripheral blood of patients with rheumatoid arthritis (RA) and the difference between them and the healthy controls. To study the relationship between PMPs level and RA activity index. Methods flow cytometry was used to detect the PMPs content in peripheral blood of 26 patients with active RA [disease activity index (DAS28 鈮，

本文編號(hào)：1633815