發(fā)布時(shí)間：2018-03-18 06:50

  本文選題：酵母源金屬硫蛋白　切入點(diǎn)：汞中毒　出處：《食品科學(xué)》2017年19期 　論文類型：期刊論文

【摘要】：目的:探討兩種具自主知識(shí)產(chǎn)權(quán)酵母源金屬硫蛋白(metallothionein,MT)(MT-1、MT-2)對慢性汞中毒小鼠排汞及汞致肝臟損傷的修復(fù)作用。方法:慢性汞中毒模型通過實(shí)驗(yàn)小鼠自由飲用氯化汞去離子水溶液構(gòu)建,經(jīng)連續(xù)灌胃給予二巰基丙磺酸鈉及不同劑量酵母源MT(0.16、0.40、0.80 mg/(kg·d))28 d后,測定實(shí)驗(yàn)小鼠體質(zhì)量、肝臟臟器系數(shù)、血液及肝臟汞含量、4項(xiàng)血常規(guī)(白細(xì)胞計(jì)數(shù)、紅細(xì)胞計(jì)數(shù)、血小板計(jì)數(shù)、血紅蛋白含量)、肝功能指示指標(biāo)并觀察慢性汞中毒小鼠肝臟組織病變程度。結(jié)果:與模型對照組比較,各灌胃給藥組小鼠體質(zhì)量均不同程度提高,肝臟臟器系數(shù)顯著下降(P0.05),血液及肝臟汞含量顯著下降(P0.05),白細(xì)胞、紅細(xì)胞、血紅蛋白含量及血小板計(jì)數(shù)均有不同程度恢復(fù),其恢復(fù)效果與劑量呈正相關(guān)。與模型對照組比較,各酵母源MT灌胃給藥組小鼠血清谷丙轉(zhuǎn)氨酶及谷草轉(zhuǎn)氨酶含量均有所下降,且綜合肝臟組織病理學(xué)染色切片結(jié)果,酵母源MT可修復(fù)汞中毒小鼠肝臟組織,使水腫肝臟細(xì)胞恢復(fù)其正常形態(tài),減少細(xì)胞間炎性浸潤現(xiàn)象,且兩種酵母源MT修復(fù)受損肝臟的能力與劑量呈正相關(guān),高劑量酵母源MT對受損肝臟的修復(fù)效果更佳。結(jié)論:綜合各項(xiàng)檢測結(jié)果,兩種酵母源MT對慢性汞中毒小鼠具有良好的排汞及修復(fù)汞致肝臟損傷效果,且酵母源MT-1對慢性汞中毒小鼠排汞及修復(fù)受損肝臟組織的效果好于酵母源MT-2及二巰基丙磺酸鈉。
[Abstract]:Objective: to investigate the effects of two kinds of metallothionein (MT-1) MT-2) on mercury excretion and mercury-induced liver injury in mice with chronic mercury poisoning. Methods: chronic mercury poisoning mice were exposed to mercuric chloride by free drinking of mercury chloride. Ionic aqueous solution construction, After continuous intragastric administration of sodium dimercaptopropylsulfonate and different doses of MT0.16 ~ 0.40 ~ 0.80 mg/(kg 路d ~ (-1), the body weight, liver organ coefficient, blood and liver mercury content of the experimental mice were measured for 4 items of blood routine (white blood cell count, red blood cell count, platelet count). Results: compared with the model control group, the body weight of the mice in each group was increased in different degree, and the content of hemoglobin and the index of liver function were also observed in the mice with chronic mercury intoxication, compared with the model control group, the content of hemoglobin and the index of liver function were measured. Liver organ coefficient decreased significantly (P 0.05), blood and liver mercury content decreased significantly (P 0.05), leukocyte, erythrocyte, hemoglobin content and platelet count recovered to varying degrees, and the recovery effect was positively correlated with dose. The contents of serum alanine aminotransferase and glutamic oxaloacetic transaminase in mice of each yeast source MT group were all decreased, and the liver tissue of mercury poisoning mice could be repaired by yeast source MT according to the results of liver histopathological staining. The edema liver cells were restored to their normal morphology and the inflammatory infiltration between cells was reduced. The ability of two kinds of yeast source MT to repair the damaged liver was positively correlated with the dose. Conclusion: two kinds of yeast source MT have good effect on removing mercury from chronic mercury poisoning mice and repairing mercury induced liver injury. The effect of yeast source MT-1 on mercury excretion and repair of damaged liver tissue in chronic mercury poisoning mice was better than that of yeast source MT-2 and sodium dimercaptopropyl sulfonate.
【作者單位】： 黑龍江八一農(nóng)墾大學(xué)食品學(xué)院;國家雜糧工程技術(shù)研究中心;青島瑯琊臺(tái)集團(tuán)股份有限公司;
【基金】：黑龍江省教育廳青年創(chuàng)新人才項(xiàng)目(2016-KYYWF-0175) 黑龍江八一農(nóng)墾大學(xué)青年創(chuàng)新人才項(xiàng)目(CXRC2016-15);黑龍江八一農(nóng)墾大學(xué)研究生創(chuàng)新科研項(xiàng)目(YJSCX2017-Y56;YJSCX2017-Y57) 黑龍江省應(yīng)用技術(shù)研究與開發(fā)計(jì)劃重大項(xiàng)目(GA15B301)
【分類號(hào)】：R595.2

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