發(fā)布時間：2018-03-18 02:21

  本文選題：糖尿病心肌病　切入點：二苯乙烯苷　出處：《中國藥理學(xué)通報》2016年03期 　論文類型：期刊論文

【摘要】：目的探討二苯乙烯苷(TSG)對糖尿病大鼠心肌損傷的作用及對沉默信息調(diào)節(jié)因子2的哺乳動物同源體1(SIRT1)和磷酸腺苷活化的蛋白激酶(AMPK)蛋白影響。方法建立2型糖尿病大鼠模型,分組給藥,16周時處死大鼠,生化法測定血糖、血脂、肝功能及肌酸激酶(CK)、乳酸脫氫酶(LDH)和心肌組織游離脂肪酸(NEFA);酶聯(lián)免疫吸附法測定心肌肌鈣蛋白Ⅰ(cTnⅠ)、心肌組織脂代謝相關(guān)酶脂肪酸跨膜轉(zhuǎn)運載體蛋白(FATPs)和脂肪酸β氧化酶(FA-β-oxidase)的含量;放射免疫法測定血漿炎癥因子腫瘤壞死因子(TNF-α)、白細胞介素6(IL-6)、白細胞介素1β(IL-1β)的含量,Western blot檢測心肌組織中TNF-α、IL-6、IL-1β、SIRT1和AMPK蛋白的表達;并測定左心室膠原含量。結(jié)果TSG干預(yù)后能減低血脂水平,對血糖和胰島素水平無明顯影響。TSG能減低糖尿病大鼠心肌組織中膠原含量,減少心臟游離脂肪酸含量,增加心肌組織脂代謝相關(guān)酶(FATPs和FA-β-oxidase)含量,抑制外周血和心肌組織中炎癥因子(TNF-α、IL-6、IL-1β)的分泌。TSG能明顯增加糖尿病大鼠心臟SIRT1和p AMPK蛋白表達。結(jié)論 TSG對糖尿病大鼠心肌具有保護作用,其機制可能與抑制心肌炎癥因子和改善能量代謝有關(guān)。
[Abstract]:Objective to investigate the effects of stilbene glucoside (TSGG) on myocardial injury in diabetic rats and its effects on mammalian homologue 1 SIRT1 (silencing signaling factor 2) and adenosine phospho-activated protein kinase (AMPK) protein. Methods Type 2 diabetic rat models were established. Rats were killed after 16 weeks of administration, and blood glucose and blood lipids were measured by biochemical method. Liver function and creatine kinase CKH, lactate dehydrogenase (LDH) and free fatty acid in myocardial tissue were determined by enzyme linked immunosorbent assay (Elisa), cardiac troponin 鈪，

本文編號：1627570