發(fā)布時(shí)間：2018-03-11 01:15

  本文選題：牙周炎　切入點(diǎn)：Ⅱ型糖尿病　出處：《山西醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的通過觀察大鼠的頸動(dòng)脈血管病理變化,比較血清炎癥因子白細(xì)胞介素-6(Interleukin-6,IL-6)水平變化,研究牙周干預(yù)對(duì)Ⅱ型糖尿病(T2DM)牙周炎(chronic periodontitis,CP)大鼠的影響?。方法44只清潔級(jí)大鼠,隨機(jī)分為A組(對(duì)照組,7只)、B組(T2DM組,8只)、C組(CP組,14只)、D組(T2DM+CP組,15只)。據(jù)是否進(jìn)行牙周干預(yù),再將C、D組隨機(jī)分為:C1/D1不干預(yù)組、C2/D2牙周干預(yù)治療組,7只/組。通過內(nèi)眥靜脈的采血方法,在干預(yù)前1周(T1)、第一次干預(yù)后1周(T2)、第二次干預(yù)后1周(T3)、3周(T4)、5周(T5)五個(gè)時(shí)間點(diǎn)采血,干預(yù)前后的血清炎癥因子IL-6的含量用酶聯(lián)免疫吸附法(ELISA)來檢測(cè),頸動(dòng)脈血管在制成石蠟切片后,HE染色,光鏡下觀察變化情況。結(jié)果1.頸動(dòng)脈病理結(jié)果顯示:A組血管形態(tài)正常;B組(T2DM組)多數(shù)頸動(dòng)脈管壁顯著增厚,內(nèi)膜表面富含炎癥細(xì)胞,較多的空泡性變,纖維排列雜亂;C/D組頸動(dòng)脈血管壁形態(tài)變化程度不一,其中C1/D1不治療組變化較大,管壁增厚明顯,泡沫、炎癥細(xì)胞廣泛的黏附存在于管壁,彈性纖維紊亂,且D1組變化程度更大;而C2/D2牙周干預(yù)組的頸動(dòng)脈管壁厚度不同,局部少量的泡沫細(xì)胞,炎癥細(xì)胞數(shù)量也較少,纖維排列有改善,而C2組頸動(dòng)脈血管狀態(tài)改善更明顯;2.血清學(xué)結(jié)果:B組IL-6含量在觀測(cè)期間是呈持續(xù)增高的走勢(shì)(P0.001);C1、D1組的IL-6在觀測(cè)期間也是持續(xù)上升的走勢(shì),且IL-6含量在最后時(shí)間點(diǎn)時(shí)分別高至(C1/D1:337.985±35.866/487.724±18.831,pg·L-1,P0.01);且D1組IL-6含量在干預(yù)后時(shí)間點(diǎn)明顯高于C1組(P0.01);與干預(yù)前各組基線相比,干預(yù)組C2、D2(除C2組第三個(gè)時(shí)間點(diǎn))IL-6的含量在干預(yù)后,隨時(shí)間的變化是呈先升后降的趨勢(shì),在干預(yù)后1周升高,此時(shí)含量分別為(C2:290.212±20.218/D2:656.802±54.232,pg·L-1),并在第三個(gè)時(shí)間點(diǎn)開始下降(P0.05);且C2/D2牙周干預(yù)組IL-6含量干預(yù)后時(shí)間點(diǎn),均低于不治療組C1/D1組相比(P0.01,除第三個(gè)時(shí)間點(diǎn)外);結(jié)論1.IL-6不僅參與了慢性牙周炎及T2DM的發(fā)生發(fā)展,且可能與血管相關(guān)病變的發(fā)生發(fā)展有關(guān)。2.若不及時(shí)控制慢性牙周炎癥,則可能會(huì)加重CP及T2DM的病變程度,促進(jìn)CP及T2DM血管病變的形成及進(jìn)展;3.牙周干預(yù)短期內(nèi)可能會(huì)增強(qiáng)機(jī)體炎癥狀態(tài),增加頸動(dòng)脈血管發(fā)生病變的風(fēng)險(xiǎn),而長(zhǎng)遠(yuǎn)效果來看則有可能會(huì)降低機(jī)體炎癥,而改善頸動(dòng)脈血管的病變程度;
[Abstract]:Objective to study the effect of periodontal intervention on chronic periodontitis (CPP) rats by observing the pathological changes of carotid artery in rats and comparing the changes of serum inflammatory factor interleukin-6 (IL-6). Methods Forty-four clean rats were randomly divided into two groups: group A (control group, n = 7), T2DM group (n = 8), group C (n = 14), group C (n = 14), group D (n = 14), and group D (n = 15) with T2DM. Group D was randomly divided into two groups: the control group (n = 7) and the control group (n = 7). Blood samples were collected from the inner canthus vein at 1 week before intervention, 1 week after the first intervention, 1 week after the first intervention, 1 week after the second intervention, 1 week after the second intervention, 3 weeks after the second intervention, 5 weeks after the intervention, and 5 weeks after the intervention, the blood samples were collected at five time points: 1 week before the intervention, 1 week after the first intervention, 1 week after the first intervention, 1 week after the second intervention, 1 week after the intervention, 5 weeks after the intervention, the blood samples were collected. The content of serum inflammatory factor IL-6 was detected by Elisa before and after intervention. The carotid artery was stained with HE after paraffin section. Results 1. The pathological results of carotid artery showed that the wall of most carotid arteries was thickened significantly, and the surface of intima was rich in inflammatory cells and vacuolar degeneration. 2. In C / D group, the changes of carotid artery wall morphology were different. In C 1 / D group, the changes of carotid artery wall were larger, the wall thickened obviously, foam and inflammatory cells were widely adhered to the wall, the elastic fiber was disordered, and the change degree of D1 group was greater than that of the control group. In the C _ 2 / D _ 2 periodontal intervention group, the thickness of carotid artery wall was different, a small number of foam cells were localized, the number of inflammatory cells was also relatively small, and the arrangement of fibers was improved. The results of serology showed that the content of IL-6 in group C _ 2 was increasing continuously during the observation period. The IL-6 of group C _ (1) C _ (1) in C _ (1) C _ (1) C _ (1) C _ (1) C _ (1) C _ (1) C _ (1) C _ (1) was also increasing during the observation period. The content of IL-6 in group D1 was significantly higher than that in group C1 at the last time point, and the content of IL-6 in group D1 was significantly higher than that in group C1 at the time point after intervention, and compared with the baseline before and after intervention, the content of IL-6 in intervention group was higher than that in group C _ 2 (except the third time point of C2 group), the content of IL-6 in group C _ 2 was significantly higher than that in group C _ 1 at the time point after intervention, and the level of IL-6 in group C _ 2 was significantly higher than that in group C _ 1 at the end of the intervention, and the level of IL-6 in group C _ 2 was significantly higher than that in group C _ 1. The content of IL-6 in the C 2 / D2 periodontal intervention group increased at 1 week after intervention, and the content was 290.212 鹵20.218D 2: 656.802 鹵54.232PG 路L -1, and decreased at the third time point, and the IL-6 content in the C 2 / D2 periodontal intervention group decreased at the time point after the intervention. Conclusion 1. IL-6 is not only involved in the occurrence and development of chronic periodontitis and T2DM, but also may be related to the occurrence and development of vascular related diseases. It may aggravate the pathological degree of CP and T2DM, promote the formation and progression of CP and T2DM vascular lesions 3.periodontal intervention may enhance the inflammatory state of the body and increase the risk of carotid artery angiopathy in a short period of time. In the long run, it is possible to reduce inflammation and improve the degree of carotid artery disease.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R781.4;R587.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李潔婷;劉玉嬌;任秀云;常樂;岳姿潔;石學(xué)雪;;口腔干預(yù)對(duì)慢性牙周炎大鼠頸動(dòng)脈基質(zhì)金屬蛋白酶2,9和血清白細(xì)胞介素6的影響[J];中華口腔醫(yī)學(xué)雜志;2014年09期

2 高秀瑩;周迎生;;2型糖尿病鼠類模型的研究進(jìn)展[J];中國(guó)實(shí)驗(yàn)動(dòng)物學(xué)報(bào);2014年04期

3 劉玉嬌;李潔婷;任秀云;常樂;岳姿潔;石學(xué)雪;;牙周治療對(duì)伴動(dòng)脈粥樣硬化牙周炎白細(xì)胞介素6及基質(zhì)金屬蛋白酶影響的動(dòng)物實(shí)驗(yàn)[J];中華口腔醫(yī)學(xué)雜志;2014年03期

4 陳蕾;蘇媛;倪佳;羅維;軒東英;章錦才;;牙周基礎(chǔ)治療對(duì)伴2型糖尿病的中、重度牙周炎患者牙周炎癥控制、血清炎癥指標(biāo)及代謝水平影響[J];華西口腔醫(yī)學(xué)雜志;2014年01期

5 任秀云;常樂;岳姿潔;林牧;石學(xué)雪;孫麗莉;;輔以抗生素的牙周機(jī)械治療對(duì)伴動(dòng)脈粥樣硬化牙周炎大鼠的頸動(dòng)脈及血清超敏C-反應(yīng)蛋白的影響[J];華西口腔醫(yī)學(xué)雜志;2013年05期

6 王雙;;探討牙周基礎(chǔ)治療對(duì)糖尿病性牙周炎的臨床效果[J];科技資訊;2013年19期

7 徐菁玲;孟煥新;李崢;李鳳云;張琳;;牙周基礎(chǔ)治療對(duì)2型糖尿病伴慢性牙周炎患者血糖代謝指標(biāo)及血清生化指標(biāo)的影響[J];北京大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2013年01期

8 陳滟;林莉;袁海潑;王凡雅;;炎癥因子與2型糖尿病血管病變研究概述[J];當(dāng)代醫(yī)學(xué);2009年07期

9 谷宇新;張金廷;李慶星;王大維;陳立X;;牙周治療對(duì)2型糖尿病患者血清白細(xì)胞介素6及糖化白蛋白的影響[J];現(xiàn)代口腔醫(yī)學(xué)雜志;2007年06期

10 郝京梅;孟煥新;紀(jì)立農(nóng);;2型糖尿病家系成員牙周狀況調(diào)查[J];中華口腔醫(yī)學(xué)雜志;2007年07期

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