本文關(guān)鍵詞： 熱打擊 谷氨酰胺 腸黏膜　出處：《解放軍醫(yī)學(xué)雜志》2017年06期 　論文類(lèi)型：期刊論文

【摘要】：目的探討谷氨酰胺(Gln)對(duì)熱打擊后腸黏膜單層上皮細(xì)胞屏障通透性改變的影響及可能機(jī)制。方法應(yīng)用Caco-2細(xì)胞株建立腸上皮機(jī)械屏障模型,加入Gln培養(yǎng)24h,43℃持續(xù)1h熱打擊。以CCK-8法檢測(cè)不同濃度Gln(0.4、0.7、1.4、2.1、2.8mmol/L)對(duì)細(xì)胞增殖的影響,為后續(xù)實(shí)驗(yàn)選擇最適合濃度;Transwell法測(cè)定單層跨膜電阻抗(TEER)值和對(duì)辣根過(guò)氧化物酶(HRP)的通透性;Western blotting檢測(cè)緊密連接蛋白o(hù)ccludin和ZO-1的表達(dá);采用考馬斯亮藍(lán)對(duì)細(xì)胞骨架進(jìn)行染色觀(guān)察。結(jié)果 0.7mmol/L的Gln促進(jìn)細(xì)胞增殖的效果最強(qiáng),與其他濃度相比,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。相較于單純43℃熱打擊組,0.7mmol/L的Gln可抑制單層上皮細(xì)胞TEER的下降和HRP通過(guò)率的升高(P0.01),增加occludin和ZO-1的表達(dá),有益于維持細(xì)胞骨架的正常結(jié)構(gòu)。結(jié)論 0.7mmol/L的Gln可減輕熱打擊對(duì)單層腸上皮細(xì)胞結(jié)構(gòu)的破壞,保護(hù)屏障功能。
[Abstract]:Objective to investigate the effect of glutamine (Glutamine) on the permeability of intestinal mucosal monolayer epithelial cell barrier after thermal shock and its possible mechanism. Methods Caco-2 cell line was used to establish the model of intestinal epithelial mechanical barrier. The effects of different concentrations of Glnn on cell proliferation were detected by CCK-8 assay. The effects of different concentrations of Glnn on cell proliferation were measured by CCK-8 assay at 43 鈩，

本文編號(hào)：1552282