本文關鍵詞： 鉛 氯碘羥喹 海馬 銅相關蛋白　出處：《環(huán)境與健康雜志》2017年06期 　論文類型：期刊論文

【摘要】：目的探討氯碘羥喹(CQ)對鉛暴露大鼠腦組織中銅相關蛋白變化的影響,為鉛中毒的修復提供理論依據(jù)。方法50只雄性SD大鼠隨機分為對照組、CQ組、鉛暴露組、鉛+CQ組及鉛+CQ+VB_(12)組,每組10只。對照組和CQ組大鼠飲用0.03%乙酸鈉飲水,其余3組大鼠飲用0.03%乙酸鉛飲水,共9周;之后CQ組、鉛+CQ組和鉛+CQ+VB_(12)組大鼠腹腔注射CQ(30 mg/kg)1周。用Morris水迷宮實驗檢測大鼠神經(jīng)行為變化,采用電感耦合等離子體質譜法測定海馬中鉛和銅水平,用ELISA方法檢測銅相關蛋白表達,以HE染色觀察海馬的病理改變。結果與對照組比較,鉛暴露組大鼠第3天的定位航行時間延長,穿臺次數(shù)減少;海馬中鉛和銅含量增加,銅鋅超氧化物歧化酶(Cu/Zn SOD)和銅藍蛋白(CP)活力下降,銅伴侶蛋白(CCS、ATOX1、COX17)表達下降,差異均有統(tǒng)計學意義(P0.05);鉛暴露大鼠海馬的神經(jīng)元細胞深染,細胞質凝膠。與鉛暴露組比較,鉛+CQ組大鼠第3天的定位航行時間、穿臺次數(shù)無明顯變化(P0.05);大鼠海馬中鉛含量下降,差異有統(tǒng)計學意義(P0.05),而銅含量無明顯變化(P0.05)。與鉛暴露組比較,鉛+CQ+VB_(12)組大鼠第3天的定位航行時間明顯縮短,穿臺次數(shù)增加,差異有統(tǒng)計學意義(P0.05);海馬中鉛和銅含量分別比鉛暴露組下降55.94%和11.29%,差異有統(tǒng)計學意義(P0.05)。鉛+CQ組和鉛+CQ+VB_(12)組大鼠海馬中CP活力均高于鉛暴露組,鉛+CQ+VB_(12)組CCS蛋白表達水平高于鉛暴露組,差異均有統(tǒng)計學意義(P0.05)。HE染色表明,CQ注射后鉛暴露組大鼠海馬中深染細胞減少。結論 CQ能夠改善大鼠認知功能,這可能與改變某些銅相關蛋白的表達有關,提示CQ可用于修復鉛中毒導致的神經(jīng)損傷。
[Abstract]:Objective to investigate the effects of chlorohydroxyquine (CQQ) on the changes of copper-associated proteins in brain tissue of lead exposed rats, and to provide theoretical basis for the repair of lead poisoning. Methods Fifty male SD rats were randomly divided into control group and lead exposure group. The rats of the control group and the CQ group drank 0.03% sodium acetate, the other three groups drank 0.03% lead acetate for 9 weeks, then the control group and the CQ group drank 0.03% lead acetate for 9 weeks. Rats in lead CQ group and lead CQ VBX group were injected intraperitoneally with CQ(30 mg/kg)1 week. The changes of neurobehavioral behavior were detected by Morris water maze test, the levels of lead and copper in hippocampus were measured by inductively coupled plasma mass spectrometry (ICP-MS), and the expression of copper-associated protein was detected by ELISA method. The pathological changes of hippocampus were observed by HE staining. Results compared with the control group, the navigation time of the rats in the lead exposure group was prolonged and the number of platform piercing decreased on the 3rd day, and the contents of lead and copper in the hippocampus increased. The activity of CuZn superoxide dismutase (Cu / Zn SOD) and ceruloplasmin (CPP) decreased, and the expression of CCS- (ATOX1) COX17) decreased, and the difference was statistically significant (P 0.05). In the third day of lead CQ group, there was no significant change in the number of platform piercing, but the content of lead in hippocampus was decreased, the difference was statistically significant (P 0.05), but the content of copper did not change significantly (P 0.05). Compared with the group exposed to lead, there was no significant change in the content of lead in the hippocampus of the CQ group, and there was no significant change in the content of copper in the group. In the lead CQ VBB12) group, the navigation time of the rats on the 3rd day was significantly shortened, and the number of platform piercing was increased. The contents of lead and copper in hippocampus were 55.94% and 11.29 lower than those in lead exposure group, respectively. The CP activity in hippocampus of lead CQ group and lead CQ VBB-1 group was higher than that of lead exposure group. The expression of CCS protein in the lead CQ VBB12) group was higher than that in the lead exposure group, and the difference was statistically significant (P 0.05) .HE staining showed that the hyperchromatic cells in the hippocampus of the lead exposure group decreased after CQ injection. Conclusion CQ can improve the cognitive function of the rats. This may be related to changes in the expression of some copper related proteins, suggesting that CQ can be used to repair nerve damage caused by lead poisoning.
【作者單位】： 華北理工大學公共衛(wèi)生學院;華北理工大學附屬醫(yī)院藥學部;
【基金】：國家自然科學基金(81373033) 河北省大學生實驗創(chuàng)新項目(201410081067)
【分類號】：R595.2

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