發(fā)布時間：2018-02-24 15:43

  本文關鍵詞： 細胞免疫學 尿酸鈉結晶 脂多糖 痛風 炎癥體 白細胞介素-β 白細胞介素-　出處：《深圳大學學報(理工版)》2016年06期 　論文類型：期刊論文

【摘要】：通過合成尿酸鈉結晶(monosodium urate crystals,MSU)分析其對人外周血單核細胞系THP1的促炎作用,分別以0、1.0、10.0和100.0μmol/L的MSU刺激THP1細胞4 h,檢測不同濃度的MSU對炎癥體組分Nod樣受體蛋白3(Nod-like receptor protein 3,NLRP3)、半胱氨酸天冬氨酸特異性蛋白水解酶(caspase-1)及促炎因子白細胞介素-1β(interleukin-1β,IL-1β)和IL-18的信使核糖核酸(messenger ribonucleic acid,mRNA)表達的影響.為研究toll樣受體4(toll like receptor 4,TLR4)的配體脂多糖的作用,用1.0 ng/m L的脂多糖(lipopolysaccharide,LPS)預刺激THP1細胞3 h,再加100.0μmol/m L的MSU刺激細胞,通過實時熒光定量聚合酶鏈式反應及酶聯(lián)免疫吸附試驗,檢測LPS對MSU誘導的促炎因子IL-1β和IL-18的mRNA及蛋白水平.研究發(fā)現(xiàn),在THP1細胞中,100.0μmol/L的MSU即可顯著激活炎癥體的mRNA,但其對促炎因子IL-1β及IL-18的mRNA表達的誘導作用卻不明顯,加入1.0 ng/m L LPS預刺激3 h后,MSU誘導的THP1細胞IL-1β和IL-18的表達量顯著增加.證明脂多糖對MSU誘導THP1細胞產(chǎn)生IL-1β和IL-18有協(xié)同作用.
[Abstract]:The effect of sodium uric acid on the inflammation of human peripheral blood monocyte cell line THP1 was analyzed by synthesizing monosodium urate crystal MSU. THP1 cells were stimulated with 0 0 渭 mol/L and 100 0 渭 mol/L MSU for 4 h, respectively. The effects of different concentrations of MSU on Nod like receptor protein 3nd-like receptor protein 3nLRP3, caspase-1 and interleukin-1 尾 interleukin-1 尾 interleukin 1 (IL-1 尾) and IL-18 were detected. In order to study the effect of ligand lipopolysaccharide (LPS) on the expression of toll like receptor 4toll like receptor 4 (TLR4), THP1 cells were prestimulated with 1. 0 ng/m / L lipopolysaccharide for 3 h and then stimulated with MSU of 100 渭 mol/m L for 3 h. Real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay (Elisa) were used. The levels of mRNA and protein of IL-1 尾 and IL-18 induced by MSU were detected by LPS. It was found that MSU of 100.0 渭 mol/L could significantly activate the expression of inflammatory mRNAs in THP1 cells, but its effect on the expression of IL-1 尾 and IL-18 in THP1 cells was not obvious. After pretreatment with 1.0 ng/m L LPS for 3 h, the expression of IL-1 尾 and IL-18 in THP1 cells induced by MSU was significantly increased, which indicated that LPS had synergistic effect on MSU induced IL-1 尾 and IL-18 production in THP1 cells.
【作者單位】： 深圳大學醫(yī)學部;深圳市人民醫(yī)院暨南大學第二臨床醫(yī)學院風濕免疫科;
【基金】：中國博士后科學基金資助項目(2015M572370) 深圳市科技計劃資助項目(JCYJ20130402092657775)~~
【分類號】：R589.7
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本文編號：1530822