發(fā)布時(shí)間：2018-02-22 21:29

  本文關(guān)鍵詞： 骨質(zhì)疏松性骨折 干細(xì)胞 生物力學(xué) 組織工程 生物材料 骨生物材料 聚乳酸共聚物 骨質(zhì)疏松骨折 大鼠脂肪干細(xì)胞 生物力學(xué)參數(shù)　出處：《中國(guó)組織工程研究》2017年10期 　論文類(lèi)型：期刊論文

【摘要】：背景:聚乳酸共聚物骨支架具有優(yōu)異的生物降解性且易被塑造為三維立體支架,能促進(jìn)骨組織和血管的生成、生長(zhǎng)。目的:觀察骨質(zhì)疏松骨折后,聚乳酸共聚物復(fù)合脂肪干細(xì)胞對(duì)骨折愈合后生物力學(xué)的影響。方法:將60只SD大鼠隨機(jī)分為4組,空白對(duì)照組不做任何處理;模型組切除雙側(cè)卵巢3個(gè)月后,制作雙側(cè)脛骨骨折模型;細(xì)胞治療組切除雙側(cè)卵巢3個(gè)月后,制作雙側(cè)脛骨骨折模型,于骨折周?chē)踩胫靖杉?xì)胞;聯(lián)合治療組切除雙側(cè)卵巢3個(gè)月后,制作雙側(cè)脛骨骨折模型,于骨折周?chē)踩刖廴樗峁簿畚?脂肪干細(xì)胞復(fù)合體。治療4周后,觀察各組骨密度、骨痂厚度、生物力學(xué)參數(shù)及骨小梁微結(jié)構(gòu)變化。結(jié)果與結(jié)論:(1)骨密度:模型組骨密度低于空白對(duì)照組(P0.05);細(xì)胞治療組、聯(lián)合治療組骨密度高于模型組(P0.05),但低于空白對(duì)照組(P0.05);聯(lián)合治療組骨密度高于細(xì)胞治療組(P0.05);(2)骨痂厚度:細(xì)胞治療組、聯(lián)合治療組骨痂厚度高于模型組、空白對(duì)照組(P0.05),且聯(lián)合治療組高于細(xì)胞治療組(P0.05);(3)生物力學(xué)測(cè)試:與空白對(duì)照組比較,模型組破壞載荷、極限應(yīng)力、剪切強(qiáng)度、彈性模量降低(P0.05),剪切應(yīng)變升高(P0.05);與模型組比較,細(xì)胞治療組、聯(lián)合治療組破壞載荷、極限應(yīng)力、剪切強(qiáng)度、彈性模量升高(P0.05),剪切應(yīng)變降低(P0.05),且以聯(lián)合治療組改變更顯著(P0.05);(4)骨小梁微結(jié)構(gòu):模型組骨小梁排列紊亂,間距增大,甚至出現(xiàn)斷裂和陷窩;細(xì)胞治療組、聯(lián)合治療組骨小梁數(shù)目增多且增粗增厚,間距減小,陷窩減少;(5)結(jié)果表明:聚乳酸共聚物復(fù)合脂肪干細(xì)胞治療骨質(zhì)疏松骨折可明顯改善愈合后骨組織的生物力學(xué)參數(shù)。
[Abstract]:Background: Polylactic acid copolymer bone scaffolds have excellent biodegradability and are easily molded as three-dimensional scaffolds, which can promote the formation and growth of bone tissue and blood vessels. Effects of Polylactic Acid Copolymer combined with adipose Stem cells on Biomechanics after fracture Healing methods: 60 SD rats were randomly divided into 4 groups without any treatment in the blank control group after 3 months of bilateral ovary resection in the model group. The model of bilateral tibial fracture was made; the model of bilateral tibial fracture was made after 3 months of bilateral ovarian resection in the cell therapy group, and adipose stem cells were implanted around the fracture; in the combined treatment group, the bilateral ovary was removed for 3 months. A bilateral tibial fracture model was established and the poly (lactic acid) copolymer-adipose stem cell complex was implanted around the fracture. After 4 weeks of treatment, bone mineral density (BMD) and callus thickness were observed in each group. Biomechanical parameters and bone trabecular microstructure.Results and conclusion: BMD in the model group was lower than that in the blank control group (P 0.05), and in the cell therapy group, the bone density in the model group was lower than that in the control group. The bone mineral density of the combined treatment group was higher than that of the model group, but lower than that of the blank control group (P 0.05); the bone mineral density of the combined treatment group was higher than that of the cell therapy group (P 0.05); the callus thickness of the combined treatment group was higher than that of the model group, and the callus thickness of the combined treatment group was higher than that of the model group. Compared with the blank control group, the damage load, ultimate stress, shear strength and elastic modulus of the model group decreased by P0.05A, and the shear strain increased by P0.05and compared with the model group. In the combined treatment group, the damage load, ultimate stress, shear strength and modulus of elasticity increased (P 0.05), shear strain decreased (P 0.05), and the bone trabecular structure was changed more significantly in the combined treatment group: the trabeculae in the model group were disordered, and the spacing was increased. In the cell therapy group, the number of trabeculae in the combined treatment group increased, the bone trabeculae became thicker and thicker, and the distance between the bone trabeculae decreased. The results showed that the biomechanical parameters of bone tissue after healing could be significantly improved by the treatment of osteoporotic fracture with poly (lactic acid) copolymer combined with adipose stem cells.
【作者單位】： 中山大學(xué)附屬第五醫(yī)院骨一科;
【基金】：廣東省醫(yī)學(xué)科研基金立項(xiàng)課題(2014A030313737)~~
【分類(lèi)號(hào)】：R580;R683
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本文編號(hào)：1525352